In Situ Electrically Resettable Field‐Effect Transistor Biosensors for Continuous and Multiplexed Neurotransmitter Detection
Abstract Accurate, real‐time detection of neurotransmitters is crucial for elucidating the mechanisms of brain function and tracking the progression of neurological diseases. Biosensors usually face poor reusability induced by difficulties in probe‐target separation, hindering their application for...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-08-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202504497 |
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| Summary: | Abstract Accurate, real‐time detection of neurotransmitters is crucial for elucidating the mechanisms of brain function and tracking the progression of neurological diseases. Biosensors usually face poor reusability induced by difficulties in probe‐target separation, hindering their application for continuous monitoring. In this work, a semiconducting carbon nanotube (CNT) film field‐effect transistor (FET) biosensor is developed using pH‐sensitive aptamers as probes to capture targets. Through tuning the potential of on‐chip palladium electrodes, the biosensor facilitates in situ pH modulation and recovery of the sensor interface, which enables an electrically resettable biosensor. The fabricated sensor demonstrates exceptional sensitivity (with femtomolar‐level detection limits), high selectivity (specific responses are 20 times stronger than non‐specific ones), and excellent reusability (over ten reuse cycles). Furthermore, in vitro detection demonstrates that the biosensor arrays, incorporating regional modifications and pH‐sensitive probes, enable the simultaneous detection of several neurotransmitters, including dopamine, serotonin, histamine, and glutamate in complex biological samples. The combination of microfluidic techniques further lowers non‐specific adsorption and cross‐reactivity, ensuring reliable, repeatable real‐time detection. The resettable CNT FET biosensor array holds significant promise for advancing the monitoring of neurotransmitter dynamics, serving as a powerful tool for the early diagnosis and management of neurological disorders. |
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| ISSN: | 2198-3844 |