Detection of fungal sequences in human brain: rDNA locus amplification and deep sequencing
Abstract The aetiology of Alzheimer’s disease (AD) and Parkinson’s disease (PD) are unknown and tend to manifest at a late stage in life; even though these neurodegenerative diseases are caused by different affected proteins, they are both characterized by neuroinflammation. Links between bacterial...
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Nature Portfolio
2024-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-82840-7 |
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| author | Rodrigo Leitao Iam Ut Wan Harry Chown Thomas J. Williams Matthew C. Fisher Johanna Rhodes |
| author_facet | Rodrigo Leitao Iam Ut Wan Harry Chown Thomas J. Williams Matthew C. Fisher Johanna Rhodes |
| author_sort | Rodrigo Leitao |
| collection | DOAJ |
| description | Abstract The aetiology of Alzheimer’s disease (AD) and Parkinson’s disease (PD) are unknown and tend to manifest at a late stage in life; even though these neurodegenerative diseases are caused by different affected proteins, they are both characterized by neuroinflammation. Links between bacterial and viral infection and AD/PD has been suggested in several studies, however, few have attempted to establish a link between fungal infection and AD/PD. In this study we adopted a nanopore-based sequencing approach to characterise the presence or absence of fungal genera in both human brain tissue and cerebrospinal fluid (CSF). We observed the presence of small fungal burden DNA in two AD brains and a control case (extensive amyloid angiopathy). This approach would be well-placed to investigate potential links between microbial infection and neurodegenerative disease. |
| format | Article |
| id | doaj-art-f0ca8543d6024952948d4b8cecfcd492 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-f0ca8543d6024952948d4b8cecfcd4922025-01-05T12:23:45ZengNature PortfolioScientific Reports2045-23222024-12-0114111110.1038/s41598-024-82840-7Detection of fungal sequences in human brain: rDNA locus amplification and deep sequencingRodrigo Leitao0Iam Ut Wan1Harry Chown2Thomas J. Williams3Matthew C. Fisher4Johanna Rhodes5MRC Centre for Global Infectious Disease Analysis, School of Public Health, Imperial College LondonMRC Centre for Global Infectious Disease Analysis, School of Public Health, Imperial College LondonMRC Centre for Global Infectious Disease Analysis, School of Public Health, Imperial College LondonDepartment of Infectious Disease, Imperial College LondonMRC Centre for Global Infectious Disease Analysis, School of Public Health, Imperial College LondonDepartment of Medical Microbiology, RadboudumcAbstract The aetiology of Alzheimer’s disease (AD) and Parkinson’s disease (PD) are unknown and tend to manifest at a late stage in life; even though these neurodegenerative diseases are caused by different affected proteins, they are both characterized by neuroinflammation. Links between bacterial and viral infection and AD/PD has been suggested in several studies, however, few have attempted to establish a link between fungal infection and AD/PD. In this study we adopted a nanopore-based sequencing approach to characterise the presence or absence of fungal genera in both human brain tissue and cerebrospinal fluid (CSF). We observed the presence of small fungal burden DNA in two AD brains and a control case (extensive amyloid angiopathy). This approach would be well-placed to investigate potential links between microbial infection and neurodegenerative disease.https://doi.org/10.1038/s41598-024-82840-7 |
| spellingShingle | Rodrigo Leitao Iam Ut Wan Harry Chown Thomas J. Williams Matthew C. Fisher Johanna Rhodes Detection of fungal sequences in human brain: rDNA locus amplification and deep sequencing Scientific Reports |
| title | Detection of fungal sequences in human brain: rDNA locus amplification and deep sequencing |
| title_full | Detection of fungal sequences in human brain: rDNA locus amplification and deep sequencing |
| title_fullStr | Detection of fungal sequences in human brain: rDNA locus amplification and deep sequencing |
| title_full_unstemmed | Detection of fungal sequences in human brain: rDNA locus amplification and deep sequencing |
| title_short | Detection of fungal sequences in human brain: rDNA locus amplification and deep sequencing |
| title_sort | detection of fungal sequences in human brain rdna locus amplification and deep sequencing |
| url | https://doi.org/10.1038/s41598-024-82840-7 |
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