Characterization of microplastics in human follicular fluid and assessment of their potential impact on mouse oocyte maturation in vitro
Microplastics (MPs) have been identified in various human tissues and organs. This study aims to evaluate the presence of MPs in human follicular fluid (hFF) and their potential impact on oocyte maturation. Laser direct infrared spectroscopy of 19 hFF samples identified 7956 particles, including 173...
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Elsevier
2025-02-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325001320 |
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| author | Danyu Ni Kaidi Yu Nan Yan Xiaojiao Chen Qijun Xie Yuqin Yang Wei Jiang Ye Yang Junqiang Zhang Xiufeng Ling |
| author_facet | Danyu Ni Kaidi Yu Nan Yan Xiaojiao Chen Qijun Xie Yuqin Yang Wei Jiang Ye Yang Junqiang Zhang Xiufeng Ling |
| author_sort | Danyu Ni |
| collection | DOAJ |
| description | Microplastics (MPs) have been identified in various human tissues and organs. This study aims to evaluate the presence of MPs in human follicular fluid (hFF) and their potential impact on oocyte maturation. Laser direct infrared spectroscopy of 19 hFF samples identified 7956 particles, including 1739 microplastics (21.9 %) from 30 types. Of these, 923 particles had matching degrees > 0.8, and 7033 had matching degrees between 0.65 and 0.8. The most abundant MPs were Chlorinated Polyethylene (CPE), Fluorosilicone rubber, Polyvinyl chloride (PVC), Butadiene rubber (BR), and Styrene-butadiene-styrene (SBS), with diameters ranging from 20 to 100 μm. Five random samples were analyzed by pyrolysis-gas chromatography-mass spectrometry, which detected four types of microplastics—Polyethylene (PE), Polypropylene (PP), Polystyrene (PS), and PVC—at varying concentrations. PE was the most abundant (22.284 mg/kg), followed by PVC (1.061 mg/kg), PP (0.837 mg/kg), and PS (0.600 mg/kg). Based on the diameter and concentration ranges of MPs in hFF, we used seven types of fluorescence-labeled MP beads—PE, PVC, PP, PS, CPE, Polymethylmethacrylate (PMMA), and Polytetrafluoroethylene (PTFE)—to assess their impact on mouse oocyte in vitro maturation. The results showed that smaller MP beads (e.g., 50 μm PE) were more likely to penetrate the zona pellucida and enter the oocyte, while larger beads (e.g., 500 nm PMMA) tended to adhere to the zona and remain outside the oocyte. All seven types of MP beads hindered oocyte maturation, resulting in varying reductions in maturation rates compared to the control group. Our findings suggest that MPs contaminate hFF and may impair oocyte maturation. |
| format | Article |
| id | doaj-art-f05a37406f5449d7a96b35380e58a6d4 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-f05a37406f5449d7a96b35380e58a6d42025-02-05T04:31:01ZengElsevierEcotoxicology and Environmental Safety0147-65132025-02-01291117796Characterization of microplastics in human follicular fluid and assessment of their potential impact on mouse oocyte maturation in vitroDanyu Ni0Kaidi Yu1Nan Yan2Xiaojiao Chen3Qijun Xie4Yuqin Yang5Wei Jiang6Ye Yang7Junqiang Zhang8Xiufeng Ling9Department of Reproductive Medicine, Women's Hospital of Nanjing Medical University, Nanjing Women and Children’s Healthcare Hospital, Nanjing, Jiangsu Province 210004, ChinaDepartment of Reproductive Medicine, Women's Hospital of Nanjing Medical University, Nanjing Women and Children’s Healthcare Hospital, Nanjing, Jiangsu Province 210004, ChinaDepartment of Reproductive Medicine, Women's Hospital of Nanjing Medical University, Nanjing Women and Children’s Healthcare Hospital, Nanjing, Jiangsu Province 210004, ChinaDepartment of Reproductive Medicine, Women's Hospital of Nanjing Medical University, Nanjing Women and Children’s Healthcare Hospital, Nanjing, Jiangsu Province 210004, ChinaDepartment of Reproductive Medicine, Women's Hospital of Nanjing Medical University, Nanjing Women and Children’s Healthcare Hospital, Nanjing, Jiangsu Province 210004, ChinaDepartment of Reproductive Medicine, Women's Hospital of Nanjing Medical University, Nanjing Women and Children’s Healthcare Hospital, Nanjing, Jiangsu Province 210004, ChinaDepartment of Reproductive Medicine, Women's Hospital of Nanjing Medical University, Nanjing Women and Children’s Healthcare Hospital, Nanjing, Jiangsu Province 210004, ChinaCorrespondence to: NO.123 Tianfei Lane, Nanjing City, Jiangsu Province 210004, China.; Department of Reproductive Medicine, Women's Hospital of Nanjing Medical University, Nanjing Women and Children’s Healthcare Hospital, Nanjing, Jiangsu Province 210004, ChinaCorrespondence to: NO.123 Tianfei Lane, Nanjing City, Jiangsu Province 210004, China.; Department of Reproductive Medicine, Women's Hospital of Nanjing Medical University, Nanjing Women and Children’s Healthcare Hospital, Nanjing, Jiangsu Province 210004, ChinaCorrespondence to: NO.123 Tianfei Lane, Nanjing City, Jiangsu Province 210004, China.; Department of Reproductive Medicine, Women's Hospital of Nanjing Medical University, Nanjing Women and Children’s Healthcare Hospital, Nanjing, Jiangsu Province 210004, ChinaMicroplastics (MPs) have been identified in various human tissues and organs. This study aims to evaluate the presence of MPs in human follicular fluid (hFF) and their potential impact on oocyte maturation. Laser direct infrared spectroscopy of 19 hFF samples identified 7956 particles, including 1739 microplastics (21.9 %) from 30 types. Of these, 923 particles had matching degrees > 0.8, and 7033 had matching degrees between 0.65 and 0.8. The most abundant MPs were Chlorinated Polyethylene (CPE), Fluorosilicone rubber, Polyvinyl chloride (PVC), Butadiene rubber (BR), and Styrene-butadiene-styrene (SBS), with diameters ranging from 20 to 100 μm. Five random samples were analyzed by pyrolysis-gas chromatography-mass spectrometry, which detected four types of microplastics—Polyethylene (PE), Polypropylene (PP), Polystyrene (PS), and PVC—at varying concentrations. PE was the most abundant (22.284 mg/kg), followed by PVC (1.061 mg/kg), PP (0.837 mg/kg), and PS (0.600 mg/kg). Based on the diameter and concentration ranges of MPs in hFF, we used seven types of fluorescence-labeled MP beads—PE, PVC, PP, PS, CPE, Polymethylmethacrylate (PMMA), and Polytetrafluoroethylene (PTFE)—to assess their impact on mouse oocyte in vitro maturation. The results showed that smaller MP beads (e.g., 50 μm PE) were more likely to penetrate the zona pellucida and enter the oocyte, while larger beads (e.g., 500 nm PMMA) tended to adhere to the zona and remain outside the oocyte. All seven types of MP beads hindered oocyte maturation, resulting in varying reductions in maturation rates compared to the control group. Our findings suggest that MPs contaminate hFF and may impair oocyte maturation.http://www.sciencedirect.com/science/article/pii/S0147651325001320Microplastic particlesHuman healthHuman exposureFollicular fluidOocyte maturation |
| spellingShingle | Danyu Ni Kaidi Yu Nan Yan Xiaojiao Chen Qijun Xie Yuqin Yang Wei Jiang Ye Yang Junqiang Zhang Xiufeng Ling Characterization of microplastics in human follicular fluid and assessment of their potential impact on mouse oocyte maturation in vitro Ecotoxicology and Environmental Safety Microplastic particles Human health Human exposure Follicular fluid Oocyte maturation |
| title | Characterization of microplastics in human follicular fluid and assessment of their potential impact on mouse oocyte maturation in vitro |
| title_full | Characterization of microplastics in human follicular fluid and assessment of their potential impact on mouse oocyte maturation in vitro |
| title_fullStr | Characterization of microplastics in human follicular fluid and assessment of their potential impact on mouse oocyte maturation in vitro |
| title_full_unstemmed | Characterization of microplastics in human follicular fluid and assessment of their potential impact on mouse oocyte maturation in vitro |
| title_short | Characterization of microplastics in human follicular fluid and assessment of their potential impact on mouse oocyte maturation in vitro |
| title_sort | characterization of microplastics in human follicular fluid and assessment of their potential impact on mouse oocyte maturation in vitro |
| topic | Microplastic particles Human health Human exposure Follicular fluid Oocyte maturation |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325001320 |
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