Effect of agarose/gelatin gel addition on the pro-angiogenic potential of polyhydroxybutyrate/chitosan scaffolds

The aim of this paper was to evaluate the effect of gel addition to biopolymeric scaffolds on the pro-angiogenic and basic material characteristics of the final composite for use in regenerative medicine. The studied scaffold consisted of natural biopolymers: polyhydroxybutyrate, chitosan, agarose,...

Full description

Saved in:
Bibliographic Details
Main Authors: Mária Giretová, Ľubomír Medvecký, Zuzana Demčišáková, Lenka Luptáková, Eva Petrovová, Radoslava Štulajterová
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-01-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fcell.2024.1504268/full
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832592441648087040
author Mária Giretová
Ľubomír Medvecký
Zuzana Demčišáková
Lenka Luptáková
Eva Petrovová
Radoslava Štulajterová
author_facet Mária Giretová
Ľubomír Medvecký
Zuzana Demčišáková
Lenka Luptáková
Eva Petrovová
Radoslava Štulajterová
author_sort Mária Giretová
collection DOAJ
description The aim of this paper was to evaluate the effect of gel addition to biopolymeric scaffolds on the pro-angiogenic and basic material characteristics of the final composite for use in regenerative medicine. The studied scaffold consisted of natural biopolymers: polyhydroxybutyrate, chitosan, agarose, and gelatin. The final scaffold was characterized by high macroporosity (90%) and wide pore size distribution. As is known, the pore size is a critical factor for cell ingrowth in grafts after implantation in the body and for angiogenic development and creation of new vessels. After 9 days of cultivation in the culture medium, the scaffold retained its physicochemical properties without any tendency of disintegration. The addition of polymeric gels to the scaffold improved the mechanical stability of the composite. In vitro cytotoxicity testing showed good adherence of the seeded L929 fibroblasts on the scaffold and strong ingrowth of cells into the macropores. No sign of cytotoxicity was identified by both the MTS assay and live/dead cell staining. The quail chorioallantoic membrane (CAM) assay—as an alternative to in vivo assays—revealed suitable pro-angiogenic properties of the scaffold for the formation and ingrowth of new blood vessels. Moreover, the upregulation of gene expression responsible for the activation of angiogenic cascade clearly demonstrated a positive effect of the prepared composites on angiogenesis as an essential part of new tissue formation and the regeneration process itself.
format Article
id doaj-art-f0531d91a68f4b6d8c769d80257dc687
institution Kabale University
issn 2296-634X
language English
publishDate 2025-01-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Cell and Developmental Biology
spelling doaj-art-f0531d91a68f4b6d8c769d80257dc6872025-01-21T08:36:50ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2025-01-011210.3389/fcell.2024.15042681504268Effect of agarose/gelatin gel addition on the pro-angiogenic potential of polyhydroxybutyrate/chitosan scaffoldsMária Giretová0Ľubomír Medvecký1Zuzana Demčišáková2Lenka Luptáková3Eva Petrovová4Radoslava Štulajterová5Division of Functional and Hybrid Materials, Institute of Materials Research of SAS, Košice, SlovakiaDivision of Functional and Hybrid Materials, Institute of Materials Research of SAS, Košice, SlovakiaDepartment of Morphological Disciplines, University of Veterinary Medicine and Pharmacy in Košice, Košice, SlovakiaDepartment of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, SlovakiaDepartment of Morphological Disciplines, University of Veterinary Medicine and Pharmacy in Košice, Košice, SlovakiaDivision of Functional and Hybrid Materials, Institute of Materials Research of SAS, Košice, SlovakiaThe aim of this paper was to evaluate the effect of gel addition to biopolymeric scaffolds on the pro-angiogenic and basic material characteristics of the final composite for use in regenerative medicine. The studied scaffold consisted of natural biopolymers: polyhydroxybutyrate, chitosan, agarose, and gelatin. The final scaffold was characterized by high macroporosity (90%) and wide pore size distribution. As is known, the pore size is a critical factor for cell ingrowth in grafts after implantation in the body and for angiogenic development and creation of new vessels. After 9 days of cultivation in the culture medium, the scaffold retained its physicochemical properties without any tendency of disintegration. The addition of polymeric gels to the scaffold improved the mechanical stability of the composite. In vitro cytotoxicity testing showed good adherence of the seeded L929 fibroblasts on the scaffold and strong ingrowth of cells into the macropores. No sign of cytotoxicity was identified by both the MTS assay and live/dead cell staining. The quail chorioallantoic membrane (CAM) assay—as an alternative to in vivo assays—revealed suitable pro-angiogenic properties of the scaffold for the formation and ingrowth of new blood vessels. Moreover, the upregulation of gene expression responsible for the activation of angiogenic cascade clearly demonstrated a positive effect of the prepared composites on angiogenesis as an essential part of new tissue formation and the regeneration process itself.https://www.frontiersin.org/articles/10.3389/fcell.2024.1504268/fullgelpolymeric scaffoldquail embryo CAM assayangiogenesiscytotoxicity
spellingShingle Mária Giretová
Ľubomír Medvecký
Zuzana Demčišáková
Lenka Luptáková
Eva Petrovová
Radoslava Štulajterová
Effect of agarose/gelatin gel addition on the pro-angiogenic potential of polyhydroxybutyrate/chitosan scaffolds
Frontiers in Cell and Developmental Biology
gel
polymeric scaffold
quail embryo CAM assay
angiogenesis
cytotoxicity
title Effect of agarose/gelatin gel addition on the pro-angiogenic potential of polyhydroxybutyrate/chitosan scaffolds
title_full Effect of agarose/gelatin gel addition on the pro-angiogenic potential of polyhydroxybutyrate/chitosan scaffolds
title_fullStr Effect of agarose/gelatin gel addition on the pro-angiogenic potential of polyhydroxybutyrate/chitosan scaffolds
title_full_unstemmed Effect of agarose/gelatin gel addition on the pro-angiogenic potential of polyhydroxybutyrate/chitosan scaffolds
title_short Effect of agarose/gelatin gel addition on the pro-angiogenic potential of polyhydroxybutyrate/chitosan scaffolds
title_sort effect of agarose gelatin gel addition on the pro angiogenic potential of polyhydroxybutyrate chitosan scaffolds
topic gel
polymeric scaffold
quail embryo CAM assay
angiogenesis
cytotoxicity
url https://www.frontiersin.org/articles/10.3389/fcell.2024.1504268/full
work_keys_str_mv AT mariagiretova effectofagarosegelatingeladditionontheproangiogenicpotentialofpolyhydroxybutyratechitosanscaffolds
AT lubomirmedvecky effectofagarosegelatingeladditionontheproangiogenicpotentialofpolyhydroxybutyratechitosanscaffolds
AT zuzanademcisakova effectofagarosegelatingeladditionontheproangiogenicpotentialofpolyhydroxybutyratechitosanscaffolds
AT lenkaluptakova effectofagarosegelatingeladditionontheproangiogenicpotentialofpolyhydroxybutyratechitosanscaffolds
AT evapetrovova effectofagarosegelatingeladditionontheproangiogenicpotentialofpolyhydroxybutyratechitosanscaffolds
AT radoslavastulajterova effectofagarosegelatingeladditionontheproangiogenicpotentialofpolyhydroxybutyratechitosanscaffolds