Detection and analysis of Serpin and RP26 specific antibodies for monitoring Schistosoma haematobium transmission.
<h4>Background</h4>Schistosoma haematobium is the causative pathogen for urogenital schistosomiasis. To achieve progress towards schistosomiasis elimination, there is a critical need for developing highly sensitive and specific tools to monitor transmission in near-elimination settings....
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-01-01
|
| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0012813 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850279857483677696 |
|---|---|
| author | Mio Kokubo-Tanaka Anna Overgaard Kildemoes Evans Asena Chadeka Benard Ngetich Cheruiyot Taeko Moriyasu Miho Sassa Risa Nakamura Mihoko Kikuchi Yoshito Fujii Claudia J de Dood Paul L A M Corstjens Satoshi Kaneko Haruhiko Maruyama Sammy M Njenga Remco de Vrueh Cornelis Hendrik Hokke Shinjiro Hamano |
| author_facet | Mio Kokubo-Tanaka Anna Overgaard Kildemoes Evans Asena Chadeka Benard Ngetich Cheruiyot Taeko Moriyasu Miho Sassa Risa Nakamura Mihoko Kikuchi Yoshito Fujii Claudia J de Dood Paul L A M Corstjens Satoshi Kaneko Haruhiko Maruyama Sammy M Njenga Remco de Vrueh Cornelis Hendrik Hokke Shinjiro Hamano |
| author_sort | Mio Kokubo-Tanaka |
| collection | DOAJ |
| description | <h4>Background</h4>Schistosoma haematobium is the causative pathogen for urogenital schistosomiasis. To achieve progress towards schistosomiasis elimination, there is a critical need for developing highly sensitive and specific tools to monitor transmission in near-elimination settings. Although antibody detection is a promising approach, it is usually unable to discriminate active infections from past ones. Moreover, crude antigens such as soluble egg antigen (SEA) show cross-reactivity with other parasitic infections, and it is difficult to formulate the standard preparations. To resolve these issues, the performances of recombinant antigens have been evaluated. The antibody responses against recombinant S. haematobium serine-protease inhibitor (ShSerpin) and RP26 were previously shown to reflect active schistosome infection in humans. Furthermore, antibody detection using multiple recombinant antigens has been reported to improve the accuracy of antibody-based assays compared to single-target assays. Therefore, we examined the performances of ShSerpin, RP26 and the mixture of these antigens for detecting S. haematobium low-intensity infection and assessed the potential for transmission monitoring.<h4>Methodology/principal findings</h4>We collected urine and plasma samples from school-aged children in Kwale, Kenya and evaluated S. haematobium prevalence by number of eggs in urine and worm-derived circulating anodic antigen (CAA) in plasma. Among 269 pupils, 50.2% were CAA-positive by the lateral flow test utilizing up-converting phosphor particles (UCP-LF CAA), while only 14.1% were egg-positive. IgG levels to S. haematobium SEA (ShSEA), ShSerpin, RP26, and the mixture of ShSerpin and RP26 were measured by ELISA. The mixture of ShSerpin and RP26 showed the highest sensitivity, 88.7%(125/141)among the four antigens in considering indecisive UCP-LF CAA results as negative.<h4>Conclusion/significance</h4>IgG detection against the ShSerpin-RP26 mixture demonstrated better sensitivity for detection of active S. haematobium infection. This recombinant antigen mixture is simpler to produce with higher reproducibility and can potentially replace ShSEA in monitoring transmission under near-elimination settings. |
| format | Article |
| id | doaj-art-f0481b4d09ff4f38bbb44090e9f5ff0e |
| institution | OA Journals |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-f0481b4d09ff4f38bbb44090e9f5ff0e2025-08-20T01:48:57ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352025-01-01191e001281310.1371/journal.pntd.0012813Detection and analysis of Serpin and RP26 specific antibodies for monitoring Schistosoma haematobium transmission.Mio Kokubo-TanakaAnna Overgaard KildemoesEvans Asena ChadekaBenard Ngetich CheruiyotTaeko MoriyasuMiho SassaRisa NakamuraMihoko KikuchiYoshito FujiiClaudia J de DoodPaul L A M CorstjensSatoshi KanekoHaruhiko MaruyamaSammy M NjengaRemco de VruehCornelis Hendrik HokkeShinjiro Hamano<h4>Background</h4>Schistosoma haematobium is the causative pathogen for urogenital schistosomiasis. To achieve progress towards schistosomiasis elimination, there is a critical need for developing highly sensitive and specific tools to monitor transmission in near-elimination settings. Although antibody detection is a promising approach, it is usually unable to discriminate active infections from past ones. Moreover, crude antigens such as soluble egg antigen (SEA) show cross-reactivity with other parasitic infections, and it is difficult to formulate the standard preparations. To resolve these issues, the performances of recombinant antigens have been evaluated. The antibody responses against recombinant S. haematobium serine-protease inhibitor (ShSerpin) and RP26 were previously shown to reflect active schistosome infection in humans. Furthermore, antibody detection using multiple recombinant antigens has been reported to improve the accuracy of antibody-based assays compared to single-target assays. Therefore, we examined the performances of ShSerpin, RP26 and the mixture of these antigens for detecting S. haematobium low-intensity infection and assessed the potential for transmission monitoring.<h4>Methodology/principal findings</h4>We collected urine and plasma samples from school-aged children in Kwale, Kenya and evaluated S. haematobium prevalence by number of eggs in urine and worm-derived circulating anodic antigen (CAA) in plasma. Among 269 pupils, 50.2% were CAA-positive by the lateral flow test utilizing up-converting phosphor particles (UCP-LF CAA), while only 14.1% were egg-positive. IgG levels to S. haematobium SEA (ShSEA), ShSerpin, RP26, and the mixture of ShSerpin and RP26 were measured by ELISA. The mixture of ShSerpin and RP26 showed the highest sensitivity, 88.7%(125/141)among the four antigens in considering indecisive UCP-LF CAA results as negative.<h4>Conclusion/significance</h4>IgG detection against the ShSerpin-RP26 mixture demonstrated better sensitivity for detection of active S. haematobium infection. This recombinant antigen mixture is simpler to produce with higher reproducibility and can potentially replace ShSEA in monitoring transmission under near-elimination settings.https://doi.org/10.1371/journal.pntd.0012813 |
| spellingShingle | Mio Kokubo-Tanaka Anna Overgaard Kildemoes Evans Asena Chadeka Benard Ngetich Cheruiyot Taeko Moriyasu Miho Sassa Risa Nakamura Mihoko Kikuchi Yoshito Fujii Claudia J de Dood Paul L A M Corstjens Satoshi Kaneko Haruhiko Maruyama Sammy M Njenga Remco de Vrueh Cornelis Hendrik Hokke Shinjiro Hamano Detection and analysis of Serpin and RP26 specific antibodies for monitoring Schistosoma haematobium transmission. PLoS Neglected Tropical Diseases |
| title | Detection and analysis of Serpin and RP26 specific antibodies for monitoring Schistosoma haematobium transmission. |
| title_full | Detection and analysis of Serpin and RP26 specific antibodies for monitoring Schistosoma haematobium transmission. |
| title_fullStr | Detection and analysis of Serpin and RP26 specific antibodies for monitoring Schistosoma haematobium transmission. |
| title_full_unstemmed | Detection and analysis of Serpin and RP26 specific antibodies for monitoring Schistosoma haematobium transmission. |
| title_short | Detection and analysis of Serpin and RP26 specific antibodies for monitoring Schistosoma haematobium transmission. |
| title_sort | detection and analysis of serpin and rp26 specific antibodies for monitoring schistosoma haematobium transmission |
| url | https://doi.org/10.1371/journal.pntd.0012813 |
| work_keys_str_mv | AT miokokubotanaka detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission AT annaovergaardkildemoes detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission AT evansasenachadeka detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission AT benardngetichcheruiyot detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission AT taekomoriyasu detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission AT mihosassa detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission AT risanakamura detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission AT mihokokikuchi detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission AT yoshitofujii detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission AT claudiajdedood detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission AT paullamcorstjens detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission AT satoshikaneko detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission AT haruhikomaruyama detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission AT sammymnjenga detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission AT remcodevrueh detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission AT cornelishendrikhokke detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission AT shinjirohamano detectionandanalysisofserpinandrp26specificantibodiesformonitoringschistosomahaematobiumtransmission |