β-lactamase genes in carbapenem resistance Acinetobacter baumannii isolates from a Turkish university hospital
Introduction: The spread of Acinetobacter baumannii, resistant to most of the available antimicrobial agents, is a serious health problem. The high rate of carbapenem resistance among Acinetobacter baumannii isolates is considered as a threat to public health. In this study, we aimed to determine t...
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The Journal of Infection in Developing Countries
2019-01-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/10556 |
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| author | Umut Safiye Say Coskun Emel Caliskan Asegul Copur Cicek Halbay Turumtay Cemal Sandalli |
| author_facet | Umut Safiye Say Coskun Emel Caliskan Asegul Copur Cicek Halbay Turumtay Cemal Sandalli |
| author_sort | Umut Safiye Say Coskun |
| collection | DOAJ |
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Introduction: The spread of Acinetobacter baumannii, resistant to most of the available antimicrobial agents, is a serious health problem. The high rate of carbapenem resistance among Acinetobacter baumannii isolates is considered as a threat to public health. In this study, we aimed to determine the antibiotic resistance and related genes in carbapenem-resistant Acinetobacter baumannii isolates.
Methodology: Ninety six isolates of A. baumannii were included. Antimicrobial susceptibility was performed by Phoenix Automated System and disk diffusion method. Carbapenem resistane was characterized by scrneeing of resistance genes such as blaTEM, blaSHV, blaCTX-M1-2, blaPER, blaVEB, blaKPC, blaGES, blaNDM, blaVIM, blaIMP and blaOXA23-24-51-58 using multiplex polymerase chain reaction.
Results: Resistance for the levofloxacin, gentamicin, amikacin, and tigecycline were determined as 96.9%, 93.7%, 72.9% and 45.8% respectively. Colistin was the only susceptible antibiotic against all clinical isolates. All isolates were defined as multidrug resistance and of these, 31.2% were extensively drug-resistant (sensitive only to colistin). BlaOXA-51 and blaOXA-23 genes were detected in 100% strains while blaTEM was found in only 2% strains. There was no amplification for the blaSHV, blaCTX-M1-2, blaPER, blaVEB, blaKPC, blaGES blaNDM, blaVIM, blaIMP and blaOXA24-58 genes.
Conclusions: The high frequency of blaOXA-23 and low frequency of blaTEM gene was observed that indicate prevalence of a variety of A. baumannii strains. The rates of resistance genes vary from region to region. Studies are required for the prevention and control of A. baumannii infection and to formulate the strategies of antibiotic usage.
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| format | Article |
| id | doaj-art-f03bfef1bdaa46fb8b32a5404f0ef03a |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-f03bfef1bdaa46fb8b32a5404f0ef03a2025-08-20T03:48:47ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802019-01-01130110.3855/jidc.10556β-lactamase genes in carbapenem resistance Acinetobacter baumannii isolates from a Turkish university hospitalUmut Safiye Say Coskun0Emel Caliskan1Asegul Copur Cicek2Halbay Turumtay3Cemal Sandalli4Department of Medical Microbiology, Faculty of Medicine, Tokat Gaziosmanpasa University, Tokat, TurkeyDepartment of Medical Microbiology, Faculty of Medicine, Duzce University, Duzce, TurkeyDepartment of Medical Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, TurkeyDepartment of Energy Systems Engineering, Faculty of Technology, Karadeniz Technical University, Trabzon, TurkeyDepartment of Biology, Faculty of Arts and Sciences, Recep Tayyip Erdogan University Rize, Turkey Introduction: The spread of Acinetobacter baumannii, resistant to most of the available antimicrobial agents, is a serious health problem. The high rate of carbapenem resistance among Acinetobacter baumannii isolates is considered as a threat to public health. In this study, we aimed to determine the antibiotic resistance and related genes in carbapenem-resistant Acinetobacter baumannii isolates. Methodology: Ninety six isolates of A. baumannii were included. Antimicrobial susceptibility was performed by Phoenix Automated System and disk diffusion method. Carbapenem resistane was characterized by scrneeing of resistance genes such as blaTEM, blaSHV, blaCTX-M1-2, blaPER, blaVEB, blaKPC, blaGES, blaNDM, blaVIM, blaIMP and blaOXA23-24-51-58 using multiplex polymerase chain reaction. Results: Resistance for the levofloxacin, gentamicin, amikacin, and tigecycline were determined as 96.9%, 93.7%, 72.9% and 45.8% respectively. Colistin was the only susceptible antibiotic against all clinical isolates. All isolates were defined as multidrug resistance and of these, 31.2% were extensively drug-resistant (sensitive only to colistin). BlaOXA-51 and blaOXA-23 genes were detected in 100% strains while blaTEM was found in only 2% strains. There was no amplification for the blaSHV, blaCTX-M1-2, blaPER, blaVEB, blaKPC, blaGES blaNDM, blaVIM, blaIMP and blaOXA24-58 genes. Conclusions: The high frequency of blaOXA-23 and low frequency of blaTEM gene was observed that indicate prevalence of a variety of A. baumannii strains. The rates of resistance genes vary from region to region. Studies are required for the prevention and control of A. baumannii infection and to formulate the strategies of antibiotic usage. https://jidc.org/index.php/journal/article/view/10556Acinetobacter baumanniimulti drug resistanceresistance genesblaOXA-23 |
| spellingShingle | Umut Safiye Say Coskun Emel Caliskan Asegul Copur Cicek Halbay Turumtay Cemal Sandalli β-lactamase genes in carbapenem resistance Acinetobacter baumannii isolates from a Turkish university hospital Journal of Infection in Developing Countries Acinetobacter baumannii multi drug resistance resistance genes blaOXA-23 |
| title | β-lactamase genes in carbapenem resistance Acinetobacter baumannii isolates from a Turkish university hospital |
| title_full | β-lactamase genes in carbapenem resistance Acinetobacter baumannii isolates from a Turkish university hospital |
| title_fullStr | β-lactamase genes in carbapenem resistance Acinetobacter baumannii isolates from a Turkish university hospital |
| title_full_unstemmed | β-lactamase genes in carbapenem resistance Acinetobacter baumannii isolates from a Turkish university hospital |
| title_short | β-lactamase genes in carbapenem resistance Acinetobacter baumannii isolates from a Turkish university hospital |
| title_sort | β lactamase genes in carbapenem resistance acinetobacter baumannii isolates from a turkish university hospital |
| topic | Acinetobacter baumannii multi drug resistance resistance genes blaOXA-23 |
| url | https://jidc.org/index.php/journal/article/view/10556 |
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