Susceptibility of lung epithelium to neutrophil elastase: protection by native inhibitors
The development of emphysema is thought to be due to an imbalance of proteases (especially neutrophil elastase [NE]) and antiproteases with loosening of the respiratory epithelium as an early event. We investigated the effect of NE on respiratory epithelial cell adherence in vitro , in the presence...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1997-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629359791488 |
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| author | L. Bingle R. J. Richards B. Fox L. Masek A. Guz T. D. Tetley |
| author_facet | L. Bingle R. J. Richards B. Fox L. Masek A. Guz T. D. Tetley |
| author_sort | L. Bingle |
| collection | DOAJ |
| description | The development of emphysema is thought to be due to an imbalance of proteases (especially neutrophil elastase [NE]) and antiproteases with loosening of the respiratory epithelium as an early event. We investigated the effect of NE on respiratory epithelial cell adherence in vitro , in the presence of varying concentrations and combinations of native inhibitors, α-1-proteinase inhibitor (PI) and secretory leukoprotease inhibitor (SLPI). SLPI was two to 12 times more effective than PI at preventing the effects of NE, especially when enzyme:inhibitor ratios were almost equivalent. Even when the concentration of SLPI was only 10% of the total (as in normal peripheral lung secretions), it gave greater protection than PI alone. This suggests that SLPI plays an important role in controlling neutrophil elastaseinduced inflammation and tissue damage. |
| format | Article |
| id | doaj-art-f035f5fd33214f2195618819cc8f939e |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1997-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-f035f5fd33214f2195618819cc8f939e2025-02-03T01:11:19ZengWileyMediators of Inflammation0962-93511466-18611997-01-0165-634535410.1080/09629359791488Susceptibility of lung epithelium to neutrophil elastase: protection by native inhibitorsL. Bingle0R. J. Richards1B. Fox2L. Masek3A. Guz4T. D. Tetley5Department of Respiratory Medicine, Imperial College School of Science Technology and Medicine at Charing Cross Hospital, Fulham Palace Road, London, UKSchool of Molecular and Medical Biosciences, University of Wales Cardiff, Cardiff, UKDepartment of Respiratory Medicine, Imperial College School of Science Technology and Medicine at Charing Cross Hospital, Fulham Palace Road, London, UKDepartment of Respiratory Medicine, Imperial College School of Science Technology and Medicine at Charing Cross Hospital, Fulham Palace Road, London, UKDepartment of Respiratory Medicine, Imperial College School of Science Technology and Medicine at Charing Cross Hospital, Fulham Palace Road, London, UKDepartment of Respiratory Medicine, Imperial College School of Science Technology and Medicine at Charing Cross Hospital, Fulham Palace Road, London, UKThe development of emphysema is thought to be due to an imbalance of proteases (especially neutrophil elastase [NE]) and antiproteases with loosening of the respiratory epithelium as an early event. We investigated the effect of NE on respiratory epithelial cell adherence in vitro , in the presence of varying concentrations and combinations of native inhibitors, α-1-proteinase inhibitor (PI) and secretory leukoprotease inhibitor (SLPI). SLPI was two to 12 times more effective than PI at preventing the effects of NE, especially when enzyme:inhibitor ratios were almost equivalent. Even when the concentration of SLPI was only 10% of the total (as in normal peripheral lung secretions), it gave greater protection than PI alone. This suggests that SLPI plays an important role in controlling neutrophil elastaseinduced inflammation and tissue damage.http://dx.doi.org/10.1080/09629359791488 |
| spellingShingle | L. Bingle R. J. Richards B. Fox L. Masek A. Guz T. D. Tetley Susceptibility of lung epithelium to neutrophil elastase: protection by native inhibitors Mediators of Inflammation |
| title | Susceptibility of lung epithelium to neutrophil elastase: protection by native inhibitors |
| title_full | Susceptibility of lung epithelium to neutrophil elastase: protection by native inhibitors |
| title_fullStr | Susceptibility of lung epithelium to neutrophil elastase: protection by native inhibitors |
| title_full_unstemmed | Susceptibility of lung epithelium to neutrophil elastase: protection by native inhibitors |
| title_short | Susceptibility of lung epithelium to neutrophil elastase: protection by native inhibitors |
| title_sort | susceptibility of lung epithelium to neutrophil elastase protection by native inhibitors |
| url | http://dx.doi.org/10.1080/09629359791488 |
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