Metarhizium anisopliae seed priming alleviates drought-induced oxidative stress and improves growth of barley (Hordeum vulgare L.)
Increasing crop resilience to drought stress through microorganisms is a sustainable approach. This study evaluated the efficacy of the endophytic fungus Metarhizium anisopliae MetA1 (MA) for improving drought tolerance of barley by analyzing various morphological, physiological, biochemical, and yi...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Plant Stress |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X24003178 |
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| author | Atofa Naz Md. Motiar Rohman Md. Ashraful Haque Mahjabin Ferdaous Mim Md. Zahid Hasan Chowdhury Razia Sultana Shah Mohammad Naimul Islam |
| author_facet | Atofa Naz Md. Motiar Rohman Md. Ashraful Haque Mahjabin Ferdaous Mim Md. Zahid Hasan Chowdhury Razia Sultana Shah Mohammad Naimul Islam |
| author_sort | Atofa Naz |
| collection | DOAJ |
| description | Increasing crop resilience to drought stress through microorganisms is a sustainable approach. This study evaluated the efficacy of the endophytic fungus Metarhizium anisopliae MetA1 (MA) for improving drought tolerance of barley by analyzing various morphological, physiological, biochemical, and yield factors. Barley grains were treated with MA (1 × 108 spore/ml) and a pot experiment was conducted with three high-yielding barley genotypes: BARI Barley-10, BARI Barley-6, and BARI Barley-9 under three drought conditions: no drought (100 % field capacity, FC), moderate drought (50 % FC), and severe drought (25 % FC). Under drought conditions, MA priming significantly enhanced shoot and root biomass, leaf characteristics, photosynthetic pigment content, and activities of various antioxidant enzymes including superoxide dismutase, catalase, glutathione S-transferase, ascorbate peroxidase, peroxidase, glutathione peroxidase, glutathione reductase, dehydroascorbate reductase, monodehydroascorbate reductase, glyoxalase-I, and glyoxalase-II in all barley genotypes. Furthermore, there was an observed increase in the levels of non-enzymatic antioxidants such as ascorbate and glutathione. MA treatment also led to a significant reduction in stress markers like methylglyoxal, malondialdehyde, lipoxygenase, hydrogen peroxide, and calcium influx along with an increase in proline and potassium content in barley leaves in stressed conditions. Crop growth and yield related attributes in barley were improved, which is evidence of better physiological and biochemical changes under both stress and non-stressed conditions. As such, the study provides evidence suggesting that MA-mediated seed priming is an effective strategy for improving drought tolerance not only in barley but also possibly other crops. |
| format | Article |
| id | doaj-art-f027ea2cb99b439eaf6eea26e0e85dfc |
| institution | DOAJ |
| issn | 2667-064X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj-art-f027ea2cb99b439eaf6eea26e0e85dfc2025-08-20T02:52:08ZengElsevierPlant Stress2667-064X2024-12-011410066410.1016/j.stress.2024.100664Metarhizium anisopliae seed priming alleviates drought-induced oxidative stress and improves growth of barley (Hordeum vulgare L.)Atofa Naz0Md. Motiar Rohman1Md. Ashraful Haque2Mahjabin Ferdaous Mim3Md. Zahid Hasan Chowdhury4Razia Sultana5Shah Mohammad Naimul Islam6Institute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman, Agricultural University, Gazipur-1706, BangladeshPlant Breeding Division, Bangladesh Agricultural Research Institute, Gazipur-1701, BangladeshInstitute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman, Agricultural University, Gazipur-1706, BangladeshInstitute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman, Agricultural University, Gazipur-1706, BangladeshInstitute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman, Agricultural University, Gazipur-1706, BangladeshDepartment of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh-2202, BangladeshInstitute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman, Agricultural University, Gazipur-1706, Bangladesh; Corresponding author.Increasing crop resilience to drought stress through microorganisms is a sustainable approach. This study evaluated the efficacy of the endophytic fungus Metarhizium anisopliae MetA1 (MA) for improving drought tolerance of barley by analyzing various morphological, physiological, biochemical, and yield factors. Barley grains were treated with MA (1 × 108 spore/ml) and a pot experiment was conducted with three high-yielding barley genotypes: BARI Barley-10, BARI Barley-6, and BARI Barley-9 under three drought conditions: no drought (100 % field capacity, FC), moderate drought (50 % FC), and severe drought (25 % FC). Under drought conditions, MA priming significantly enhanced shoot and root biomass, leaf characteristics, photosynthetic pigment content, and activities of various antioxidant enzymes including superoxide dismutase, catalase, glutathione S-transferase, ascorbate peroxidase, peroxidase, glutathione peroxidase, glutathione reductase, dehydroascorbate reductase, monodehydroascorbate reductase, glyoxalase-I, and glyoxalase-II in all barley genotypes. Furthermore, there was an observed increase in the levels of non-enzymatic antioxidants such as ascorbate and glutathione. MA treatment also led to a significant reduction in stress markers like methylglyoxal, malondialdehyde, lipoxygenase, hydrogen peroxide, and calcium influx along with an increase in proline and potassium content in barley leaves in stressed conditions. Crop growth and yield related attributes in barley were improved, which is evidence of better physiological and biochemical changes under both stress and non-stressed conditions. As such, the study provides evidence suggesting that MA-mediated seed priming is an effective strategy for improving drought tolerance not only in barley but also possibly other crops.http://www.sciencedirect.com/science/article/pii/S2667064X24003178Antioxidant enzymesBarleyDroughtMetarhizium anisopliaeOxidative damage |
| spellingShingle | Atofa Naz Md. Motiar Rohman Md. Ashraful Haque Mahjabin Ferdaous Mim Md. Zahid Hasan Chowdhury Razia Sultana Shah Mohammad Naimul Islam Metarhizium anisopliae seed priming alleviates drought-induced oxidative stress and improves growth of barley (Hordeum vulgare L.) Plant Stress Antioxidant enzymes Barley Drought Metarhizium anisopliae Oxidative damage |
| title | Metarhizium anisopliae seed priming alleviates drought-induced oxidative stress and improves growth of barley (Hordeum vulgare L.) |
| title_full | Metarhizium anisopliae seed priming alleviates drought-induced oxidative stress and improves growth of barley (Hordeum vulgare L.) |
| title_fullStr | Metarhizium anisopliae seed priming alleviates drought-induced oxidative stress and improves growth of barley (Hordeum vulgare L.) |
| title_full_unstemmed | Metarhizium anisopliae seed priming alleviates drought-induced oxidative stress and improves growth of barley (Hordeum vulgare L.) |
| title_short | Metarhizium anisopliae seed priming alleviates drought-induced oxidative stress and improves growth of barley (Hordeum vulgare L.) |
| title_sort | metarhizium anisopliae seed priming alleviates drought induced oxidative stress and improves growth of barley hordeum vulgare l |
| topic | Antioxidant enzymes Barley Drought Metarhizium anisopliae Oxidative damage |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X24003178 |
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