Fine particulate matter (PM2.5) induces microRNA-192–5p causing glomerular damage
An association between air pollution and the incidence of membranous glomerulonephritis (MGN) has been shown in epidemiological studies. However, the causality of this relationship and data on potential pathomechanisms are still missing. Anti-phospholipase A2 receptor (PLA2R1) antibodies, upregulati...
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Elsevier
2025-06-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325006165 |
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| author | Melina Yarbakht George Sarau Yanyi Xu Xinyi Fang Zeynab Mirzaei René Krüger Yalan Xiao Arslan Usman Christoph Daniel Mario Schiffer Silke Christiansen Janina Müller-Deile |
| author_facet | Melina Yarbakht George Sarau Yanyi Xu Xinyi Fang Zeynab Mirzaei René Krüger Yalan Xiao Arslan Usman Christoph Daniel Mario Schiffer Silke Christiansen Janina Müller-Deile |
| author_sort | Melina Yarbakht |
| collection | DOAJ |
| description | An association between air pollution and the incidence of membranous glomerulonephritis (MGN) has been shown in epidemiological studies. However, the causality of this relationship and data on potential pathomechanisms are still missing. Anti-phospholipase A2 receptor (PLA2R1) antibodies, upregulation of microRNA-192–5p, and decreased expression of its podocyte target nephronectin (NPNT) in patients with MGN have been shown, but the trigger for these regulations remained unknown. The current study aimed to assay the possible role of PM2.5 in the pathogenesis of MGN. In this study, we characterized particulate matter (PM2.5) collected on air filters in Shanghai by scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray fluorescence, microwave plasma atomic emission spectroscopy, nanoparticle tracking analysis, and Raman spectroscopy. Cultured human podocytes, zebrafish, and mice were exposed to PM2.5 to assess possible effects on glomerular function and ultrastructure. PM2.5 caused a reduction of podocyte-specific markers and upregulation of microRNA-192–5p. Moreover, NPNT/npnt/Npnt were downregulated, while PLA2R1/pla2r1/Pla2r1 were upregulated. PM2.5 was able to cause edema, proteinuria, and glomerular damage with loosening of the glomerular basement membrane and partial podocyte effacement in zebrafish larvae. BulkRNA seq analysis and qPCR of zebrafish larvae showed an increase in inflammatory response and oxidative stress due to the exposure to PM2.5. Long-term exposure of mice to ambient PM2.5 induced glomerular damage, albuminuria, and upregulation of pulmonary microRNA-192–5p. Therefore, air pollution might be involved in developing MGN through inflammatory pathways and the induction of microRNA-192–5p, which targets gene expression important for glomerular cell function. |
| format | Article |
| id | doaj-art-eff51d9b2a7c424db95eed26f9381cd0 |
| institution | OA Journals |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-eff51d9b2a7c424db95eed26f9381cd02025-08-20T01:53:27ZengElsevierEcotoxicology and Environmental Safety0147-65132025-06-0129811828010.1016/j.ecoenv.2025.118280Fine particulate matter (PM2.5) induces microRNA-192–5p causing glomerular damageMelina Yarbakht0George Sarau1Yanyi Xu2Xinyi Fang3Zeynab Mirzaei4René Krüger5Yalan Xiao6Arslan Usman7Christoph Daniel8Mario Schiffer9Silke Christiansen10Janina Müller-Deile11Department of Nephrology and Hypertension, Uniklinikum Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, GermanyFraunhofer Institute for Ceramic Technologies and Systems IKTS, Dresden, Germany; Leuchs Emeritus Group, Max Planck Institute for the Science of Light, Erlangen, Germany; Institute for Nanotechnology and Correlative Microscopy eV INAM, Forchheim, GermanyDepartment of Environmental Health, School of Public Health, Fudan University, Shanghai ChinaDepartment of Environmental Health, School of Public Health, Fudan University, Shanghai ChinaInstitute for Nanotechnology and Correlative Microscopy eV INAM, Forchheim, GermanyDepartment of Nephrology and Hypertension, Uniklinikum Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, GermanyDepartment of Environmental Health, School of Public Health, Fudan University, Shanghai ChinaFraunhofer Institute for Ceramic Technologies and Systems IKTS, Dresden, GermanyDepartment of Nephropathology, Uniklinikum Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, GermanyDepartment of Nephrology and Hypertension, Uniklinikum Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, GermanyFraunhofer Institute for Ceramic Technologies and Systems IKTS, Dresden, Germany; Leuchs Emeritus Group, Max Planck Institute for the Science of Light, Erlangen, Germany; Institute for Nanotechnology and Correlative Microscopy eV INAM, Forchheim, Germany; Physics Department, Freie Universität Berlin, Berlin, GermanyDepartment of Nephrology and Hypertension, Uniklinikum Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, Germany; Corresponding author.An association between air pollution and the incidence of membranous glomerulonephritis (MGN) has been shown in epidemiological studies. However, the causality of this relationship and data on potential pathomechanisms are still missing. Anti-phospholipase A2 receptor (PLA2R1) antibodies, upregulation of microRNA-192–5p, and decreased expression of its podocyte target nephronectin (NPNT) in patients with MGN have been shown, but the trigger for these regulations remained unknown. The current study aimed to assay the possible role of PM2.5 in the pathogenesis of MGN. In this study, we characterized particulate matter (PM2.5) collected on air filters in Shanghai by scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray fluorescence, microwave plasma atomic emission spectroscopy, nanoparticle tracking analysis, and Raman spectroscopy. Cultured human podocytes, zebrafish, and mice were exposed to PM2.5 to assess possible effects on glomerular function and ultrastructure. PM2.5 caused a reduction of podocyte-specific markers and upregulation of microRNA-192–5p. Moreover, NPNT/npnt/Npnt were downregulated, while PLA2R1/pla2r1/Pla2r1 were upregulated. PM2.5 was able to cause edema, proteinuria, and glomerular damage with loosening of the glomerular basement membrane and partial podocyte effacement in zebrafish larvae. BulkRNA seq analysis and qPCR of zebrafish larvae showed an increase in inflammatory response and oxidative stress due to the exposure to PM2.5. Long-term exposure of mice to ambient PM2.5 induced glomerular damage, albuminuria, and upregulation of pulmonary microRNA-192–5p. Therefore, air pollution might be involved in developing MGN through inflammatory pathways and the induction of microRNA-192–5p, which targets gene expression important for glomerular cell function.http://www.sciencedirect.com/science/article/pii/S0147651325006165Air pollutionPM2.5glomerulusproteinuriazebrafish modelpodocytes |
| spellingShingle | Melina Yarbakht George Sarau Yanyi Xu Xinyi Fang Zeynab Mirzaei René Krüger Yalan Xiao Arslan Usman Christoph Daniel Mario Schiffer Silke Christiansen Janina Müller-Deile Fine particulate matter (PM2.5) induces microRNA-192–5p causing glomerular damage Ecotoxicology and Environmental Safety Air pollution PM2.5 glomerulus proteinuria zebrafish model podocytes |
| title | Fine particulate matter (PM2.5) induces microRNA-192–5p causing glomerular damage |
| title_full | Fine particulate matter (PM2.5) induces microRNA-192–5p causing glomerular damage |
| title_fullStr | Fine particulate matter (PM2.5) induces microRNA-192–5p causing glomerular damage |
| title_full_unstemmed | Fine particulate matter (PM2.5) induces microRNA-192–5p causing glomerular damage |
| title_short | Fine particulate matter (PM2.5) induces microRNA-192–5p causing glomerular damage |
| title_sort | fine particulate matter pm2 5 induces microrna 192 5p causing glomerular damage |
| topic | Air pollution PM2.5 glomerulus proteinuria zebrafish model podocytes |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325006165 |
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