Taxonomic characterization and secondary metabolite production of newly isolated Streptomyces sp. MC12
An actinobacterium newly isolated from soil during a screening study was identified as Streptomyces sp. MC12 (GenBank accession number: PP757795) based on 16S rRNA analysis. For secondary metabolite production, fermentation was carried out in ISP 2 broth at 30°C, pH 7.3, for seven days under shaking...
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2024-11-01
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| Series: | International Journal of Secondary Metabolite |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/3918444 |
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| author | Mustafa Oskay |
| author_facet | Mustafa Oskay |
| author_sort | Mustafa Oskay |
| collection | DOAJ |
| description | An actinobacterium newly isolated from soil during a screening study was identified as Streptomyces sp. MC12 (GenBank accession number: PP757795) based on 16S rRNA analysis. For secondary metabolite production, fermentation was carried out in ISP 2 broth at 30°C, pH 7.3, for seven days under shaking conditions at 180 rpm. As a result of fermentation studies, the antagonistic effect of the crude extract, obtained through ethyl acetate extraction, against various microorganisms was determined. The MIC values of the extract against Staphylococcus aureus and Escherichia coli were 101.3 µg/mL and 153.6 µg/mL, respectively. It was also found to exhibit strong antifungal activity against Penicillium spp. Streptomyces sp. MC12, which displays both antifungal and antibacterial properties, is considered a potential secondary metabolite producer for future studies, particularly in pharmacology and the biocontrol of fungal pathogens. |
| format | Article |
| id | doaj-art-efe206ee087e48639e80d8fcedbc4704 |
| institution | OA Journals |
| issn | 2148-6905 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | izzet kara |
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| series | International Journal of Secondary Metabolite |
| spelling | doaj-art-efe206ee087e48639e80d8fcedbc47042025-08-20T02:22:03Zengizzet karaInternational Journal of Secondary Metabolite2148-69052024-11-0111474075010.21448/ijsm.1481362618Taxonomic characterization and secondary metabolite production of newly isolated Streptomyces sp. MC12Mustafa Oskay0https://orcid.org/0000-0001-8693-5621MANİSA CELÂL BAYAR ÜNİVERSİTESİAn actinobacterium newly isolated from soil during a screening study was identified as Streptomyces sp. MC12 (GenBank accession number: PP757795) based on 16S rRNA analysis. For secondary metabolite production, fermentation was carried out in ISP 2 broth at 30°C, pH 7.3, for seven days under shaking conditions at 180 rpm. As a result of fermentation studies, the antagonistic effect of the crude extract, obtained through ethyl acetate extraction, against various microorganisms was determined. The MIC values of the extract against Staphylococcus aureus and Escherichia coli were 101.3 µg/mL and 153.6 µg/mL, respectively. It was also found to exhibit strong antifungal activity against Penicillium spp. Streptomyces sp. MC12, which displays both antifungal and antibacterial properties, is considered a potential secondary metabolite producer for future studies, particularly in pharmacology and the biocontrol of fungal pathogens.https://dergipark.org.tr/en/download/article-file/3918444fermentationidentificationsecondary metabolitestreptomyces sp.16s rrna sequencingfermentationidentificationsecondary metabolitestreptomyces sp.16s rrna sequencing |
| spellingShingle | Mustafa Oskay Taxonomic characterization and secondary metabolite production of newly isolated Streptomyces sp. MC12 International Journal of Secondary Metabolite fermentation identification secondary metabolite streptomyces sp. 16s rrna sequencing fermentation identification secondary metabolite streptomyces sp. 16s rrna sequencing |
| title | Taxonomic characterization and secondary metabolite production of newly isolated Streptomyces sp. MC12 |
| title_full | Taxonomic characterization and secondary metabolite production of newly isolated Streptomyces sp. MC12 |
| title_fullStr | Taxonomic characterization and secondary metabolite production of newly isolated Streptomyces sp. MC12 |
| title_full_unstemmed | Taxonomic characterization and secondary metabolite production of newly isolated Streptomyces sp. MC12 |
| title_short | Taxonomic characterization and secondary metabolite production of newly isolated Streptomyces sp. MC12 |
| title_sort | taxonomic characterization and secondary metabolite production of newly isolated streptomyces sp mc12 |
| topic | fermentation identification secondary metabolite streptomyces sp. 16s rrna sequencing fermentation identification secondary metabolite streptomyces sp. 16s rrna sequencing |
| url | https://dergipark.org.tr/en/download/article-file/3918444 |
| work_keys_str_mv | AT mustafaoskay taxonomiccharacterizationandsecondarymetaboliteproductionofnewlyisolatedstreptomycesspmc12 |