Heterologous Expression of Type II PKS Gene Cluster Leads to Diversified Angucyclines in <i>Streptomyces albus</i> J1074

Heterologous Expression of Type II PKS Gene Cluster Leads to Diversified Angucyclines in <i>Streptomyces albus</i> J1074

Heterologous expression has emerged as an effective strategy in activating <i>Streptomyces</i> cryptic gene clusters or improving yield. Eight compounds were successfully obtained by heterologous expression of the type II PKS gene cluster <i>spi</i> derived from marine <i&...

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Bibliographic Details
Main Authors: Xiaoting Zhang, Falei Zhang, Chen Li, Jiayi Li, Xiao Xu, Tianjiao Zhu, Qian Che, Deihai Li, Guojian Zhang
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Marine Drugs
Subjects:
heterologous expression
II PKS
marine <i>Streptomyces</i>
angucycline
Online Access:https://www.mdpi.com/1660-3397/22/11/480
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Summary:Heterologous expression has emerged as an effective strategy in activating <i>Streptomyces</i> cryptic gene clusters or improving yield. Eight compounds were successfully obtained by heterologous expression of the type II PKS gene cluster <i>spi</i> derived from marine <i>Streptomyces</i> sp. HDN155000 in the chassis host <i>Streptomyces albus</i> J1074. The structures with absolute configurations were elucidated using extensive MS and NMR spectroscopic methods, as well as theoretical NMR calculations and electronic circular dichroism (ECD) calculations. Interestingly, compound WS009 Z (<b>2</b>) contains a rare thiomethyl group, angumycinone T (<b>4</b>) has a novel oxo-bridge formed between C12a and C4, and angumycinone X (<b>3</b>) showed cytotoxicity toward K562 and NCI-H446/EP cell lines.
ISSN:1660-3397

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