DISRUPTION OF EXPRESSION OF MRNA HSP27 AND UBIQUITIN AS A MECHANISM OF ESCAPING OF JURKAT LINE TUMOR CELLS FROM APOTOSIS
The research objective is to establish the link between heat shock protein 27 and ubiquitin mRNA expression as well as Jukart tumor cell apoptosis.The method of flow cytofluorometry has been used to evaluate apoptosis realization using FITC-labeled annexin V and propidium iodide along with the amoun...
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| Format: | Article |
| Language: | English |
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Siberian State Medical University (Tomsk)
2015-02-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/118 |
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| author | O. L. Nosareva Ye. A. Stepovaya N. V. Ryazantseva Ye. V. Zakirova I. O. Mazunin L. S. Litvinova N. A. Sokhonevich O. N. Vesnina Ye. V. Shakhristova |
| author_facet | O. L. Nosareva Ye. A. Stepovaya N. V. Ryazantseva Ye. V. Zakirova I. O. Mazunin L. S. Litvinova N. A. Sokhonevich O. N. Vesnina Ye. V. Shakhristova |
| author_sort | O. L. Nosareva |
| collection | DOAJ |
| description | The research objective is to establish the link between heat shock protein 27 and ubiquitin mRNA expression as well as Jukart tumor cell apoptosis.The method of flow cytofluorometry has been used to evaluate apoptosis realization using FITC-labeled annexin V and propidium iodide along with the amount of reactive oxygen species. Spectrofluorimetry has been applied to register the caspase-3 activity. The content of hydroxyl radicals has been determined by spectrophotometry. The level of ubiquitin and heat shock protein 27 mRNA expression has been identified using real-time PCR. Intact Jukart tumor cells and blood lymphocytes of healthy donors served the material for the research.Following the carried out research it has been found out that the fall in the amount of annexin V positive cells and the reduced caspase-3 activity were accompanied by the rise in the content of hydroxyl radicals and reactive oxygen species against the backdrop of the increased heat shock protein 27 and ubiquitin mRNA expression in Jukart tumor cells. |
| format | Article |
| id | doaj-art-efda5a52c99c4d22bd0240306e967fa0 |
| institution | Kabale University |
| issn | 1682-0363 1819-3684 |
| language | English |
| publishDate | 2015-02-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj-art-efda5a52c99c4d22bd0240306e967fa02025-08-20T04:00:12ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842015-02-01141667210.20538/1682-0363-2015-1-66-72116DISRUPTION OF EXPRESSION OF MRNA HSP27 AND UBIQUITIN AS A MECHANISM OF ESCAPING OF JURKAT LINE TUMOR CELLS FROM APOTOSISO. L. Nosareva0Ye. A. Stepovaya1N. V. Ryazantseva2Ye. V. Zakirova3I. O. Mazunin4L. S. Litvinova5N. A. Sokhonevich6O. N. Vesnina7Ye. V. Shakhristova8Сибирский государственный медицинский университет, ТомскSiberian State Medical University, TomskSiberian State Medical University, TomskSiberian State Medical University, TomskImmanuel Kant Baltic Federal University, KaliningradImmanuel Kant Baltic Federal University, KaliningradImmanuel Kant Baltic Federal University, KaliningradSiberian State Medical University, TomskSiberian State Medical University, TomskThe research objective is to establish the link between heat shock protein 27 and ubiquitin mRNA expression as well as Jukart tumor cell apoptosis.The method of flow cytofluorometry has been used to evaluate apoptosis realization using FITC-labeled annexin V and propidium iodide along with the amount of reactive oxygen species. Spectrofluorimetry has been applied to register the caspase-3 activity. The content of hydroxyl radicals has been determined by spectrophotometry. The level of ubiquitin and heat shock protein 27 mRNA expression has been identified using real-time PCR. Intact Jukart tumor cells and blood lymphocytes of healthy donors served the material for the research.Following the carried out research it has been found out that the fall in the amount of annexin V positive cells and the reduced caspase-3 activity were accompanied by the rise in the content of hydroxyl radicals and reactive oxygen species against the backdrop of the increased heat shock protein 27 and ubiquitin mRNA expression in Jukart tumor cells.https://bulletin.ssmu.ru/jour/article/view/118jukart tumor cellsapoptosisubiquitinheat shock protein 27 |
| spellingShingle | O. L. Nosareva Ye. A. Stepovaya N. V. Ryazantseva Ye. V. Zakirova I. O. Mazunin L. S. Litvinova N. A. Sokhonevich O. N. Vesnina Ye. V. Shakhristova DISRUPTION OF EXPRESSION OF MRNA HSP27 AND UBIQUITIN AS A MECHANISM OF ESCAPING OF JURKAT LINE TUMOR CELLS FROM APOTOSIS Бюллетень сибирской медицины jukart tumor cells apoptosis ubiquitin heat shock protein 27 |
| title | DISRUPTION OF EXPRESSION OF MRNA HSP27 AND UBIQUITIN AS A MECHANISM OF ESCAPING OF JURKAT LINE TUMOR CELLS FROM APOTOSIS |
| title_full | DISRUPTION OF EXPRESSION OF MRNA HSP27 AND UBIQUITIN AS A MECHANISM OF ESCAPING OF JURKAT LINE TUMOR CELLS FROM APOTOSIS |
| title_fullStr | DISRUPTION OF EXPRESSION OF MRNA HSP27 AND UBIQUITIN AS A MECHANISM OF ESCAPING OF JURKAT LINE TUMOR CELLS FROM APOTOSIS |
| title_full_unstemmed | DISRUPTION OF EXPRESSION OF MRNA HSP27 AND UBIQUITIN AS A MECHANISM OF ESCAPING OF JURKAT LINE TUMOR CELLS FROM APOTOSIS |
| title_short | DISRUPTION OF EXPRESSION OF MRNA HSP27 AND UBIQUITIN AS A MECHANISM OF ESCAPING OF JURKAT LINE TUMOR CELLS FROM APOTOSIS |
| title_sort | disruption of expression of mrna hsp27 and ubiquitin as a mechanism of escaping of jurkat line tumor cells from apotosis |
| topic | jukart tumor cells apoptosis ubiquitin heat shock protein 27 |
| url | https://bulletin.ssmu.ru/jour/article/view/118 |
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