Seasonal Dynamics of Eukaryotic Microbial Communities in the Water-Receiving Reservoir of the Long-Distance Water Diversion Project, China

Inter-basin water transfer projects, such as the Yellow River to Qingdao Water Diversion Project (YQWD), are essential for addressing water scarcity, but impact local aquatic ecosystems. This study investigates the seasonal characteristics of eukaryotic microbial communities in the Jihongtan Reservo...

Full description

Saved in:
Bibliographic Details
Main Authors: Yingying Yang, Fangfang Ci, Ailing Xu, Xijian Zhang, Ning Ding, Nianxin Wan, Yuanyuan Lv, Zhiwen Song
Format: Article
Language:English
Published: MDPI AG 2024-09-01
Series:Microorganisms
Subjects:
Online Access:https://www.mdpi.com/2076-2607/12/9/1873
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850260232166440960
author Yingying Yang
Fangfang Ci
Ailing Xu
Xijian Zhang
Ning Ding
Nianxin Wan
Yuanyuan Lv
Zhiwen Song
author_facet Yingying Yang
Fangfang Ci
Ailing Xu
Xijian Zhang
Ning Ding
Nianxin Wan
Yuanyuan Lv
Zhiwen Song
author_sort Yingying Yang
collection DOAJ
description Inter-basin water transfer projects, such as the Yellow River to Qingdao Water Diversion Project (YQWD), are essential for addressing water scarcity, but impact local aquatic ecosystems. This study investigates the seasonal characteristics of eukaryotic microbial communities in the Jihongtan Reservoir, the main water-receiving body of YQWD, over a one-year period using 18S rDNA amplicon sequencing. The results showed that the eukaryotic microbial diversity did not exhibit significant seasonal variation (<i>p</i> > 0.05), but there was a notable variance in the community structure (<i>p</i> < 0.05). Arthropoda and <i>Paracyclopina</i>, representing the most dominant phylum and the most dominant genus, respectively, both exhibited the lowest abundance during the winter. The Chlorophyta, as the second-dominant phylum, demonstrates its higher abundance in the spring and winter. The Mantel test and PLS-PM (Partial Least Squares Path Modeling) revealed that water temperature (WT), dissolved oxygen (DO), and pH influenced the seasonal dynamic of eukaryotic microbial communities significantly, of which WT was the primary driving factor. In addition to environmental factors, water diversion is likely to be an important influencing factor. The results of the co-occurrence network and robustness suggested that the spring network is the most complex and exhibits the highest stability. Moreover, keystone taxa within networks have been identified, revealing that these key groups encompass both abundant and rare species, with specificity to different seasons. These insights are vital for understanding the seasonal variation of microbial communities in the Jihongtan Reservoir during ongoing water diversions.
format Article
id doaj-art-efd74a3e0c2d492791f5adbe4d9b6023
institution OA Journals
issn 2076-2607
language English
publishDate 2024-09-01
publisher MDPI AG
record_format Article
series Microorganisms
spelling doaj-art-efd74a3e0c2d492791f5adbe4d9b60232025-08-20T01:55:41ZengMDPI AGMicroorganisms2076-26072024-09-01129187310.3390/microorganisms12091873Seasonal Dynamics of Eukaryotic Microbial Communities in the Water-Receiving Reservoir of the Long-Distance Water Diversion Project, ChinaYingying Yang0Fangfang Ci1Ailing Xu2Xijian Zhang3Ning Ding4Nianxin Wan5Yuanyuan Lv6Zhiwen Song7School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266520, ChinaQingdao Branch of Shandong Water Transfer Project Operation and Maintenance Center, Qingdao 266525, ChinaSchool of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266520, ChinaBinzhou Branch of Shandong Water Transfer Project Operation and Maintenance Center, Binzhou 256600, ChinaSchool of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266520, ChinaQingdao Branch of Shandong Water Transfer Project Operation and Maintenance Center, Qingdao 266525, ChinaSchool of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266520, ChinaSchool of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266520, ChinaInter-basin water transfer projects, such as the Yellow River to Qingdao Water Diversion Project (YQWD), are essential for addressing water scarcity, but impact local aquatic ecosystems. This study investigates the seasonal characteristics of eukaryotic microbial communities in the Jihongtan Reservoir, the main water-receiving body of YQWD, over a one-year period using 18S rDNA amplicon sequencing. The results showed that the eukaryotic microbial diversity did not exhibit significant seasonal variation (<i>p</i> > 0.05), but there was a notable variance in the community structure (<i>p</i> < 0.05). Arthropoda and <i>Paracyclopina</i>, representing the most dominant phylum and the most dominant genus, respectively, both exhibited the lowest abundance during the winter. The Chlorophyta, as the second-dominant phylum, demonstrates its higher abundance in the spring and winter. The Mantel test and PLS-PM (Partial Least Squares Path Modeling) revealed that water temperature (WT), dissolved oxygen (DO), and pH influenced the seasonal dynamic of eukaryotic microbial communities significantly, of which WT was the primary driving factor. In addition to environmental factors, water diversion is likely to be an important influencing factor. The results of the co-occurrence network and robustness suggested that the spring network is the most complex and exhibits the highest stability. Moreover, keystone taxa within networks have been identified, revealing that these key groups encompass both abundant and rare species, with specificity to different seasons. These insights are vital for understanding the seasonal variation of microbial communities in the Jihongtan Reservoir during ongoing water diversions.https://www.mdpi.com/2076-2607/12/9/1873eukaryotic microorganismsstorage reservoirseasonal dynamicsenvironmental driving factorsco-occurrence network patterns
spellingShingle Yingying Yang
Fangfang Ci
Ailing Xu
Xijian Zhang
Ning Ding
Nianxin Wan
Yuanyuan Lv
Zhiwen Song
Seasonal Dynamics of Eukaryotic Microbial Communities in the Water-Receiving Reservoir of the Long-Distance Water Diversion Project, China
Microorganisms
eukaryotic microorganisms
storage reservoir
seasonal dynamics
environmental driving factors
co-occurrence network patterns
title Seasonal Dynamics of Eukaryotic Microbial Communities in the Water-Receiving Reservoir of the Long-Distance Water Diversion Project, China
title_full Seasonal Dynamics of Eukaryotic Microbial Communities in the Water-Receiving Reservoir of the Long-Distance Water Diversion Project, China
title_fullStr Seasonal Dynamics of Eukaryotic Microbial Communities in the Water-Receiving Reservoir of the Long-Distance Water Diversion Project, China
title_full_unstemmed Seasonal Dynamics of Eukaryotic Microbial Communities in the Water-Receiving Reservoir of the Long-Distance Water Diversion Project, China
title_short Seasonal Dynamics of Eukaryotic Microbial Communities in the Water-Receiving Reservoir of the Long-Distance Water Diversion Project, China
title_sort seasonal dynamics of eukaryotic microbial communities in the water receiving reservoir of the long distance water diversion project china
topic eukaryotic microorganisms
storage reservoir
seasonal dynamics
environmental driving factors
co-occurrence network patterns
url https://www.mdpi.com/2076-2607/12/9/1873
work_keys_str_mv AT yingyingyang seasonaldynamicsofeukaryoticmicrobialcommunitiesinthewaterreceivingreservoirofthelongdistancewaterdiversionprojectchina
AT fangfangci seasonaldynamicsofeukaryoticmicrobialcommunitiesinthewaterreceivingreservoirofthelongdistancewaterdiversionprojectchina
AT ailingxu seasonaldynamicsofeukaryoticmicrobialcommunitiesinthewaterreceivingreservoirofthelongdistancewaterdiversionprojectchina
AT xijianzhang seasonaldynamicsofeukaryoticmicrobialcommunitiesinthewaterreceivingreservoirofthelongdistancewaterdiversionprojectchina
AT ningding seasonaldynamicsofeukaryoticmicrobialcommunitiesinthewaterreceivingreservoirofthelongdistancewaterdiversionprojectchina
AT nianxinwan seasonaldynamicsofeukaryoticmicrobialcommunitiesinthewaterreceivingreservoirofthelongdistancewaterdiversionprojectchina
AT yuanyuanlv seasonaldynamicsofeukaryoticmicrobialcommunitiesinthewaterreceivingreservoirofthelongdistancewaterdiversionprojectchina
AT zhiwensong seasonaldynamicsofeukaryoticmicrobialcommunitiesinthewaterreceivingreservoirofthelongdistancewaterdiversionprojectchina