Self-propagating wave drives morphogenesis of skull bones in vivo

Abstract Cellular motion is a key feature of tissue morphogenesis and is often driven by migration. However, migration need not explain cell motion in contexts where there is little free space or no obvious substrate, such as those found during organogenesis of mesenchymal organs including the embry...

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Main Authors: Yiteng Dang, Johanna Lattner, Adrian A. Lahola-Chomiak, Diana Alves Afonso, Elke Ulbricht, Anna Taubenberger, Steffen Rulands, Jacqueline M. Tabler
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-59164-9
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author Yiteng Dang
Johanna Lattner
Adrian A. Lahola-Chomiak
Diana Alves Afonso
Elke Ulbricht
Anna Taubenberger
Steffen Rulands
Jacqueline M. Tabler
author_facet Yiteng Dang
Johanna Lattner
Adrian A. Lahola-Chomiak
Diana Alves Afonso
Elke Ulbricht
Anna Taubenberger
Steffen Rulands
Jacqueline M. Tabler
author_sort Yiteng Dang
collection DOAJ
description Abstract Cellular motion is a key feature of tissue morphogenesis and is often driven by migration. However, migration need not explain cell motion in contexts where there is little free space or no obvious substrate, such as those found during organogenesis of mesenchymal organs including the embryonic skull. Through ex vivo imaging, biophysical modeling, and perturbation experiments, we find that mechanical feedback between cell fate and stiffness drives bone expansion and controls bone size in vivo. This mechanical feedback system is sufficient to propagate a wave of differentiation that establishes a collagen gradient which we find sufficient to describe patterns of osteoblast motion. Our work provides a mechanism for coordinated motion that may not rely upon cell migration but on emergent properties of the mesenchymal collective. Identification of such alternative mechanisms of mechanochemical coupling between differentiation and morphogenesis will help in understanding how directed cellular motility arises in complex environments with inhomogeneous material properties.
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spelling doaj-art-efca3b6b3f184fd0b0fc33739bf40bb12025-08-20T03:09:19ZengNature PortfolioNature Communications2041-17232025-05-0116111110.1038/s41467-025-59164-9Self-propagating wave drives morphogenesis of skull bones in vivoYiteng Dang0Johanna Lattner1Adrian A. Lahola-Chomiak2Diana Alves Afonso3Elke Ulbricht4Anna Taubenberger5Steffen Rulands6Jacqueline M. Tabler7Max Planck Institute for Molecular Cell Biology and GeneticsMax Planck Institute for Molecular Cell Biology and GeneticsMax Planck Institute for Molecular Cell Biology and GeneticsMax Planck Institute for Molecular Cell Biology and GeneticsBiotechnology Center (BIOTEC)Biotechnology Center (BIOTEC)Max Planck Institute for the Physics of Complex SystemsMax Planck Institute for Molecular Cell Biology and GeneticsAbstract Cellular motion is a key feature of tissue morphogenesis and is often driven by migration. However, migration need not explain cell motion in contexts where there is little free space or no obvious substrate, such as those found during organogenesis of mesenchymal organs including the embryonic skull. Through ex vivo imaging, biophysical modeling, and perturbation experiments, we find that mechanical feedback between cell fate and stiffness drives bone expansion and controls bone size in vivo. This mechanical feedback system is sufficient to propagate a wave of differentiation that establishes a collagen gradient which we find sufficient to describe patterns of osteoblast motion. Our work provides a mechanism for coordinated motion that may not rely upon cell migration but on emergent properties of the mesenchymal collective. Identification of such alternative mechanisms of mechanochemical coupling between differentiation and morphogenesis will help in understanding how directed cellular motility arises in complex environments with inhomogeneous material properties.https://doi.org/10.1038/s41467-025-59164-9
spellingShingle Yiteng Dang
Johanna Lattner
Adrian A. Lahola-Chomiak
Diana Alves Afonso
Elke Ulbricht
Anna Taubenberger
Steffen Rulands
Jacqueline M. Tabler
Self-propagating wave drives morphogenesis of skull bones in vivo
Nature Communications
title Self-propagating wave drives morphogenesis of skull bones in vivo
title_full Self-propagating wave drives morphogenesis of skull bones in vivo
title_fullStr Self-propagating wave drives morphogenesis of skull bones in vivo
title_full_unstemmed Self-propagating wave drives morphogenesis of skull bones in vivo
title_short Self-propagating wave drives morphogenesis of skull bones in vivo
title_sort self propagating wave drives morphogenesis of skull bones in vivo
url https://doi.org/10.1038/s41467-025-59164-9
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