ROLE OF MULTIPLEX CYTOKINE ANALYSIS IN THE EVALUATION OF THE EFFICACY OF RITUXIMAB DURING TREATMENT FOR RHEUMATOID ARTHRITIS

Along with its basic activity in removing B-lymphocytes, rituximab (RTM) causes depletion of a population of CD20+ T cells that can pro- duce a variety of immunoregulatory and proinflammatory cytokines and chemokines. Objective: to define a role of multiplex cytokine analysis in the evaluation of th...

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Main Authors: A. A. Novikov, E. N. Aleksandrova, T. V. Popkova, O. G. Lineva, A. S. Avdeyeva, D. S. Novikova, G. Kh. Kuzikyants, G. V. Lukina, Ya. A. Sigidin, A. V. Karaulov, S. N. Bykovskaya, E. L. Nasonov
Format: Article
Language:Russian
Published: IMA PRESS LLC 2011-06-01
Series:Научно-практическая ревматология
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Online Access:https://rsp.mediar-press.net/rsp/article/view/1293
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author A. A. Novikov
E. N. Aleksandrova
T. V. Popkova
O. G. Lineva
A. S. Avdeyeva
D. S. Novikova
G. Kh. Kuzikyants
G. V. Lukina
Ya. A. Sigidin
A. V. Karaulov
S. N. Bykovskaya
E. L. Nasonov
author_facet A. A. Novikov
E. N. Aleksandrova
T. V. Popkova
O. G. Lineva
A. S. Avdeyeva
D. S. Novikova
G. Kh. Kuzikyants
G. V. Lukina
Ya. A. Sigidin
A. V. Karaulov
S. N. Bykovskaya
E. L. Nasonov
author_sort A. A. Novikov
collection DOAJ
description Along with its basic activity in removing B-lymphocytes, rituximab (RTM) causes depletion of a population of CD20+ T cells that can pro- duce a variety of immunoregulatory and proinflammatory cytokines and chemokines. Objective: to define a role of multiplex cytokine analysis in the evaluation of the efficiency of using RMT in rheumatoid arthritis (RA). Subjects and methods. Thirty-four patients with the valid diagnosis of RA according to the ACR criteria of 1987 were examined. The con- centrations of cytokines were measured using the xMAP technology (27-plex). Results and discussion. In the group of patients with a clinical response to therapy with the gene engineering biological agent, there was a decrease in the concentrations of interleukins (IL) 1β, 1ra, 2, 4, 6, 9, and 13, granulocyte macrophage colony-stimulating factor (GM- CSF), γ-interferon (IFN-γ), monocyte chemoattractant 1 at week 8 of therapy; that in IL 1β, 1ra, 2, 5, 6, 9, 10, 12, 13, and 15, fibroblast growth factor 2 (FGF-2), GM-CSF, IFN-γ, and tumor necrosis factor-α at week 24, and that in IL-9 at week 40. The no-clinical response group showed a reduction in GM-CSF at week 8 and in IL-2 and macrophage inflammatory protein 1β (MIP-1β) at week 40, and an increase in IL-8 at week 8. At week 8 after drug infusion, the elevated levels of IL-17 and MIP-1β can be identified as possible early pre- dictors of a response (at week 40). Comparison of the baseline cytokine levels in the groups with different clinical response demonstrated a more than three-fold increase in the concentrations of IL 4, 5, 7, 8, 10, 12, 13, 15, 17, IFN-γ, and vascular endothelial growth factor, and IL-8 at weeks 8 and 40, respectively.
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series Научно-практическая ревматология
spelling doaj-art-efaf01e084ea427cb8ffeb4b08bbe9142025-08-20T03:38:12ZrusIMA PRESS LLCНаучно-практическая ревматология1995-44841995-44922011-06-01495515710.14412/1995-4484-2011-14611233ROLE OF MULTIPLEX CYTOKINE ANALYSIS IN THE EVALUATION OF THE EFFICACY OF RITUXIMAB DURING TREATMENT FOR RHEUMATOID ARTHRITISA. A. Novikov0E. N. AleksandrovaT. V. PopkovaO. G. LinevaA. S. AvdeyevaD. S. NovikovaG. Kh. KuzikyantsG. V. LukinaYa. A. SigidinA. V. KaraulovS. N. BykovskayaE. L. Nasonovаleksandrovaen@irramn.ruAlong with its basic activity in removing B-lymphocytes, rituximab (RTM) causes depletion of a population of CD20+ T cells that can pro- duce a variety of immunoregulatory and proinflammatory cytokines and chemokines. Objective: to define a role of multiplex cytokine analysis in the evaluation of the efficiency of using RMT in rheumatoid arthritis (RA). Subjects and methods. Thirty-four patients with the valid diagnosis of RA according to the ACR criteria of 1987 were examined. The con- centrations of cytokines were measured using the xMAP technology (27-plex). Results and discussion. In the group of patients with a clinical response to therapy with the gene engineering biological agent, there was a decrease in the concentrations of interleukins (IL) 1β, 1ra, 2, 4, 6, 9, and 13, granulocyte macrophage colony-stimulating factor (GM- CSF), γ-interferon (IFN-γ), monocyte chemoattractant 1 at week 8 of therapy; that in IL 1β, 1ra, 2, 5, 6, 9, 10, 12, 13, and 15, fibroblast growth factor 2 (FGF-2), GM-CSF, IFN-γ, and tumor necrosis factor-α at week 24, and that in IL-9 at week 40. The no-clinical response group showed a reduction in GM-CSF at week 8 and in IL-2 and macrophage inflammatory protein 1β (MIP-1β) at week 40, and an increase in IL-8 at week 8. At week 8 after drug infusion, the elevated levels of IL-17 and MIP-1β can be identified as possible early pre- dictors of a response (at week 40). Comparison of the baseline cytokine levels in the groups with different clinical response demonstrated a more than three-fold increase in the concentrations of IL 4, 5, 7, 8, 10, 12, 13, 15, 17, IFN-γ, and vascular endothelial growth factor, and IL-8 at weeks 8 and 40, respectively.https://rsp.mediar-press.net/rsp/article/view/1293rheumatoid arthritisrituximabcytokinesmultiplex analysis
spellingShingle A. A. Novikov
E. N. Aleksandrova
T. V. Popkova
O. G. Lineva
A. S. Avdeyeva
D. S. Novikova
G. Kh. Kuzikyants
G. V. Lukina
Ya. A. Sigidin
A. V. Karaulov
S. N. Bykovskaya
E. L. Nasonov
ROLE OF MULTIPLEX CYTOKINE ANALYSIS IN THE EVALUATION OF THE EFFICACY OF RITUXIMAB DURING TREATMENT FOR RHEUMATOID ARTHRITIS
Научно-практическая ревматология
rheumatoid arthritis
rituximab
cytokines
multiplex analysis
title ROLE OF MULTIPLEX CYTOKINE ANALYSIS IN THE EVALUATION OF THE EFFICACY OF RITUXIMAB DURING TREATMENT FOR RHEUMATOID ARTHRITIS
title_full ROLE OF MULTIPLEX CYTOKINE ANALYSIS IN THE EVALUATION OF THE EFFICACY OF RITUXIMAB DURING TREATMENT FOR RHEUMATOID ARTHRITIS
title_fullStr ROLE OF MULTIPLEX CYTOKINE ANALYSIS IN THE EVALUATION OF THE EFFICACY OF RITUXIMAB DURING TREATMENT FOR RHEUMATOID ARTHRITIS
title_full_unstemmed ROLE OF MULTIPLEX CYTOKINE ANALYSIS IN THE EVALUATION OF THE EFFICACY OF RITUXIMAB DURING TREATMENT FOR RHEUMATOID ARTHRITIS
title_short ROLE OF MULTIPLEX CYTOKINE ANALYSIS IN THE EVALUATION OF THE EFFICACY OF RITUXIMAB DURING TREATMENT FOR RHEUMATOID ARTHRITIS
title_sort role of multiplex cytokine analysis in the evaluation of the efficacy of rituximab during treatment for rheumatoid arthritis
topic rheumatoid arthritis
rituximab
cytokines
multiplex analysis
url https://rsp.mediar-press.net/rsp/article/view/1293
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