Toll-like receptor upregulation in liver and peripheral blood mononuclear cells of patients with amoebic liver abscess
Aim: We aimed to understand the host and microbe interactions at the time of infection and inflammatory response in amoebic liver abscess (ALA) patients based on toll-like receptor (TLR) expression (mRNA), cytokine and IgG subtypes levels. Methods and results: Liver aspirates from 100 ALA patients a...
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Elsevier
2025-03-01
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| Series: | Immunobiology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0171298525000038 |
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| author | Sandhya Khunger Abhishek Mewara Upninder Kaur Ajay Duseja Pallab Ray Naveen Kalra Navneet Sharma Rakesh Sehgal |
| author_facet | Sandhya Khunger Abhishek Mewara Upninder Kaur Ajay Duseja Pallab Ray Naveen Kalra Navneet Sharma Rakesh Sehgal |
| author_sort | Sandhya Khunger |
| collection | DOAJ |
| description | Aim: We aimed to understand the host and microbe interactions at the time of infection and inflammatory response in amoebic liver abscess (ALA) patients based on toll-like receptor (TLR) expression (mRNA), cytokine and IgG subtypes levels. Methods and results: Liver aspirates from 100 ALA patients and 11 liver autopsy samples were used as negative controls. Blood samples from 100 ALA and 41 healthy individuals were collected. mRNA expression of TLR 1 to 9 genes was measured using reverse transcriptase polymerase chain reaction (RT-PCR). Serum cytokines level was quantified by flow cytometry. In-house ELISA for the analysis of IgG and its subtypes in the serum samples was performed. A total of 7 TLR genes (TLR1, TLR2, TLR4, TLR6, TLR7, TLR8 and TLR9) and 6 TLR genes (TLR1, TLR2, TLR3, TLR4, TLR5 and TLR8) were found to be elevated in liver aspirates and PBMCs respectively. Increased serum cytokine levels were observed in ALA patients vs. healthy controls. Interestingly, a significant increase in IgG and its subtypes (IgG1, IgG3 and IgG4) was found in the serum of ALA patients. Conclusion: Increased levels of TLR, pro- and anti-inflammatory cytokines, IgG and its subtypes, are possibly linked with early-stage infection in ALA patients. Impact statement: The role of TLRs in association with ALA might provide insights into new therapeutic strategies. |
| format | Article |
| id | doaj-art-efaaf5973e8e4e19b7949d530f51641f |
| institution | Kabale University |
| issn | 0171-2985 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Immunobiology |
| spelling | doaj-art-efaaf5973e8e4e19b7949d530f51641f2025-02-08T04:59:25ZengElsevierImmunobiology0171-29852025-03-012302152869Toll-like receptor upregulation in liver and peripheral blood mononuclear cells of patients with amoebic liver abscessSandhya Khunger0Abhishek Mewara1Upninder Kaur2Ajay Duseja3Pallab Ray4Naveen Kalra5Navneet Sharma6Rakesh Sehgal7Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India; Department of Microbiology (FAHS), Shree Guru Gobind Singh Tricentenary (SGT) University, Gurugram, (Haryana) 122505,IndiaDepartment of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, IndiaDepartment of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, IndiaDepartment of Hepatology, Postgraduate Institute of Medical Education and Research, Chandigarh, IndiaDepartment of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, IndiaDepartment of Radiodiagnosis and Imaging, Postgraduate Institute of Medical Education and Research, Chandigarh, IndiaDepartment of Internal Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, IndiaDepartment of Microbiology, Aarupadai Veed Medical College and Hospital, Vinayaka Mission's Research Foundation- DU, Pondicherry, India; Corresponding Author at: Aarupadai veed medical college and hospital, Vinayaka Mission's Research Foundation- DU, Pondicherry, India.Aim: We aimed to understand the host and microbe interactions at the time of infection and inflammatory response in amoebic liver abscess (ALA) patients based on toll-like receptor (TLR) expression (mRNA), cytokine and IgG subtypes levels. Methods and results: Liver aspirates from 100 ALA patients and 11 liver autopsy samples were used as negative controls. Blood samples from 100 ALA and 41 healthy individuals were collected. mRNA expression of TLR 1 to 9 genes was measured using reverse transcriptase polymerase chain reaction (RT-PCR). Serum cytokines level was quantified by flow cytometry. In-house ELISA for the analysis of IgG and its subtypes in the serum samples was performed. A total of 7 TLR genes (TLR1, TLR2, TLR4, TLR6, TLR7, TLR8 and TLR9) and 6 TLR genes (TLR1, TLR2, TLR3, TLR4, TLR5 and TLR8) were found to be elevated in liver aspirates and PBMCs respectively. Increased serum cytokine levels were observed in ALA patients vs. healthy controls. Interestingly, a significant increase in IgG and its subtypes (IgG1, IgG3 and IgG4) was found in the serum of ALA patients. Conclusion: Increased levels of TLR, pro- and anti-inflammatory cytokines, IgG and its subtypes, are possibly linked with early-stage infection in ALA patients. Impact statement: The role of TLRs in association with ALA might provide insights into new therapeutic strategies.http://www.sciencedirect.com/science/article/pii/S0171298525000038Toll-like receptorPeripheral blood mononuclear cellAmoebic liver abscessPyogenic liver abscess |
| spellingShingle | Sandhya Khunger Abhishek Mewara Upninder Kaur Ajay Duseja Pallab Ray Naveen Kalra Navneet Sharma Rakesh Sehgal Toll-like receptor upregulation in liver and peripheral blood mononuclear cells of patients with amoebic liver abscess Immunobiology Toll-like receptor Peripheral blood mononuclear cell Amoebic liver abscess Pyogenic liver abscess |
| title | Toll-like receptor upregulation in liver and peripheral blood mononuclear cells of patients with amoebic liver abscess |
| title_full | Toll-like receptor upregulation in liver and peripheral blood mononuclear cells of patients with amoebic liver abscess |
| title_fullStr | Toll-like receptor upregulation in liver and peripheral blood mononuclear cells of patients with amoebic liver abscess |
| title_full_unstemmed | Toll-like receptor upregulation in liver and peripheral blood mononuclear cells of patients with amoebic liver abscess |
| title_short | Toll-like receptor upregulation in liver and peripheral blood mononuclear cells of patients with amoebic liver abscess |
| title_sort | toll like receptor upregulation in liver and peripheral blood mononuclear cells of patients with amoebic liver abscess |
| topic | Toll-like receptor Peripheral blood mononuclear cell Amoebic liver abscess Pyogenic liver abscess |
| url | http://www.sciencedirect.com/science/article/pii/S0171298525000038 |
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