Effect of dexamethasone on carrageenin-induced inflammation in the lung
To study the anti-inflammatory mechanisms of glucocorticoids, we have compared the effects of intratracheal carrageenin (2.5 mg) on control rats and those in which inflammation was subdued by prior dexamethasone treatment (10 mg/l in drinking water). Inflammation was maximal 48 h post-carrageenin. A...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1995-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935195000445 |
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| author | S. F. Smith A. Benjamin A. Dewar M. Sheppard B. Fox T. Smith A. Guz T. D. Tetley |
| author_facet | S. F. Smith A. Benjamin A. Dewar M. Sheppard B. Fox T. Smith A. Guz T. D. Tetley |
| author_sort | S. F. Smith |
| collection | DOAJ |
| description | To study the anti-inflammatory mechanisms of glucocorticoids, we have compared the effects of intratracheal carrageenin (2.5 mg) on control rats and those in which inflammation was subdued by prior dexamethasone treatment (10 mg/l in drinking water). Inflammation was maximal 48 h post-carrageenin. After dexamethasone, carrageenin caused tittle inflammation or oedema (wet lung (mg), n = 6, mean ± S.E.M.; control, 995 ± 51; carrageenin + dexamethasone, 1144 ± 83; compared with carrageenin alone, 1881 ± 198), but rats had more lung lavage neutrophils than those given carrageenin alone (PMN × 106 /lung, mean ± S.E.M.; control, 0.055 ± 0.003; carrageenin + dexamethasone, 8.54 ± 1.52; compared with carrageenin alone, 6.30 ± 1.71). Proteolysis and partial inactivation of the anti-inflammatory mediator, lipocortin 1 (Lcl), in carrageenin-instilled rats was offset in those also given dexamethasone, by increased Lc1 levels (intact Lc1 ng/ml lavage fluid, n = 4, mean ± S.E.M.; control 24 ± 6; carrageenin 15 ± 4; carrageenin + dexamethasone, 40 ± 15). Maintenance of sufficient intact (fully active) extracellular Lc1 may contribute to the actions of glucocorticoids. |
| format | Article |
| id | doaj-art-efaa4c9553d344a78da46a28591b4841 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1995-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-efaa4c9553d344a78da46a28591b48412025-08-20T02:22:05ZengWileyMediators of Inflammation0962-93511466-18611995-01-014427328110.1155/S0962935195000445Effect of dexamethasone on carrageenin-induced inflammation in the lungS. F. Smith0A. Benjamin1A. Dewar2M. Sheppard3B. Fox4T. Smith5A. Guz6T. D. Tetley7Department of Medicine, Charing Cross and Westminster Medical School, Fulham Palace Road, London W6 8RF, UKDepartment of Medicine, Charing Cross and Westminster Medical School, Fulham Palace Road, London W6 8RF, UKElectron Microscopy Unit, Royal Brompton Hospital, Sydney Street, London SW3 6NP, UKDepartment of Histopathology, Royal Brompton Hospital, Sydney Street, London SW3 6NP, UKDepartment of Histopathology, Charing Cross and Westminster Medical School, Fulham Palace Road, London W6 8RF, UKDepartment of Medicine, Charing Cross and Westminster Medical School, Fulham Palace Road, London W6 8RF, UKDepartment of Medicine, Charing Cross and Westminster Medical School, Fulham Palace Road, London W6 8RF, UKDepartment of Medicine, Charing Cross and Westminster Medical School, Fulham Palace Road, London W6 8RF, UKTo study the anti-inflammatory mechanisms of glucocorticoids, we have compared the effects of intratracheal carrageenin (2.5 mg) on control rats and those in which inflammation was subdued by prior dexamethasone treatment (10 mg/l in drinking water). Inflammation was maximal 48 h post-carrageenin. After dexamethasone, carrageenin caused tittle inflammation or oedema (wet lung (mg), n = 6, mean ± S.E.M.; control, 995 ± 51; carrageenin + dexamethasone, 1144 ± 83; compared with carrageenin alone, 1881 ± 198), but rats had more lung lavage neutrophils than those given carrageenin alone (PMN × 106 /lung, mean ± S.E.M.; control, 0.055 ± 0.003; carrageenin + dexamethasone, 8.54 ± 1.52; compared with carrageenin alone, 6.30 ± 1.71). Proteolysis and partial inactivation of the anti-inflammatory mediator, lipocortin 1 (Lcl), in carrageenin-instilled rats was offset in those also given dexamethasone, by increased Lc1 levels (intact Lc1 ng/ml lavage fluid, n = 4, mean ± S.E.M.; control 24 ± 6; carrageenin 15 ± 4; carrageenin + dexamethasone, 40 ± 15). Maintenance of sufficient intact (fully active) extracellular Lc1 may contribute to the actions of glucocorticoids.http://dx.doi.org/10.1155/S0962935195000445 |
| spellingShingle | S. F. Smith A. Benjamin A. Dewar M. Sheppard B. Fox T. Smith A. Guz T. D. Tetley Effect of dexamethasone on carrageenin-induced inflammation in the lung Mediators of Inflammation |
| title | Effect of dexamethasone on carrageenin-induced inflammation in the lung |
| title_full | Effect of dexamethasone on carrageenin-induced inflammation in the lung |
| title_fullStr | Effect of dexamethasone on carrageenin-induced inflammation in the lung |
| title_full_unstemmed | Effect of dexamethasone on carrageenin-induced inflammation in the lung |
| title_short | Effect of dexamethasone on carrageenin-induced inflammation in the lung |
| title_sort | effect of dexamethasone on carrageenin induced inflammation in the lung |
| url | http://dx.doi.org/10.1155/S0962935195000445 |
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