Detection of Powdery Mildew Resistance Gene in Melon Cultivar Meloni Based on SCAR Markers
Powdery mildew is one of the diseases caused by fungal infections that can reduce the production of melon fruit worldwide including in Indonesia. A powdery mildew-resistant cultivar of melon is needed to increase melon yield crops. This study aimed to detect resistance gene linked to powdery mildew...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Negeri Semarang
2020-04-01
|
| Series: | Biosaintifika: Journal of Biology & Biology Education |
| Subjects: | |
| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/22198 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849738791794769920 |
|---|---|
| author | Muhammad Alif Ishak Budi Setiadi Daryono |
| author_facet | Muhammad Alif Ishak Budi Setiadi Daryono |
| author_sort | Muhammad Alif Ishak |
| collection | DOAJ |
| description | Powdery mildew is one of the diseases caused by fungal infections that can reduce the production of melon fruit worldwide including in Indonesia. A powdery mildew-resistant cultivar of melon is needed to increase melon yield crops. This study aimed to detect resistance gene linked to powdery mildew using a sequence characterized amplified region (SCAR) markers. The melon cultivar Meloni was used in this study. SL-3, PI 371795, and Aramis cultivar were used to compare. Amplification of the marker was performed employing a pair of primers. The result showed that Meloni had a powdery mildew resistance gene by the presence of a DNA target band at 1058 base pair (bp). Based on this result, it could be concluded that Meloni was an excellent melon cultivar because of its ability to overcome the powdery mildew infections naturally. SCAR markers have been used for various purposes, especially to detect resistance genes to plant diseases. The present study had provided information for plant breeders about Meloni as the new melon cultivar that was genetically resistant against powdery mildew infections. Furthermore, Meloni could be proposed as an alternative to native Indonesian superior melon seeds. |
| format | Article |
| id | doaj-art-efa760f0c4554806bc09b5ddee0db810 |
| institution | DOAJ |
| issn | 2085-191X 2338-7610 |
| language | English |
| publishDate | 2020-04-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj-art-efa760f0c4554806bc09b5ddee0db8102025-08-20T03:06:27ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102020-04-01121768210.15294/biosaintifika.v12i1.221989585Detection of Powdery Mildew Resistance Gene in Melon Cultivar Meloni Based on SCAR MarkersMuhammad Alif Ishak0Budi Setiadi Daryono1Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah MadaLaboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah MadaPowdery mildew is one of the diseases caused by fungal infections that can reduce the production of melon fruit worldwide including in Indonesia. A powdery mildew-resistant cultivar of melon is needed to increase melon yield crops. This study aimed to detect resistance gene linked to powdery mildew using a sequence characterized amplified region (SCAR) markers. The melon cultivar Meloni was used in this study. SL-3, PI 371795, and Aramis cultivar were used to compare. Amplification of the marker was performed employing a pair of primers. The result showed that Meloni had a powdery mildew resistance gene by the presence of a DNA target band at 1058 base pair (bp). Based on this result, it could be concluded that Meloni was an excellent melon cultivar because of its ability to overcome the powdery mildew infections naturally. SCAR markers have been used for various purposes, especially to detect resistance genes to plant diseases. The present study had provided information for plant breeders about Meloni as the new melon cultivar that was genetically resistant against powdery mildew infections. Furthermore, Meloni could be proposed as an alternative to native Indonesian superior melon seeds.https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/22198meloni, powdery mildew, resistance gene, scar |
| spellingShingle | Muhammad Alif Ishak Budi Setiadi Daryono Detection of Powdery Mildew Resistance Gene in Melon Cultivar Meloni Based on SCAR Markers Biosaintifika: Journal of Biology & Biology Education meloni, powdery mildew, resistance gene, scar |
| title | Detection of Powdery Mildew Resistance Gene in Melon Cultivar Meloni Based on SCAR Markers |
| title_full | Detection of Powdery Mildew Resistance Gene in Melon Cultivar Meloni Based on SCAR Markers |
| title_fullStr | Detection of Powdery Mildew Resistance Gene in Melon Cultivar Meloni Based on SCAR Markers |
| title_full_unstemmed | Detection of Powdery Mildew Resistance Gene in Melon Cultivar Meloni Based on SCAR Markers |
| title_short | Detection of Powdery Mildew Resistance Gene in Melon Cultivar Meloni Based on SCAR Markers |
| title_sort | detection of powdery mildew resistance gene in melon cultivar meloni based on scar markers |
| topic | meloni, powdery mildew, resistance gene, scar |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/22198 |
| work_keys_str_mv | AT muhammadalifishak detectionofpowderymildewresistancegeneinmeloncultivarmelonibasedonscarmarkers AT budisetiadidaryono detectionofpowderymildewresistancegeneinmeloncultivarmelonibasedonscarmarkers |