Level of fecal neopterin and calprotectin in asymptomatic and symptomatic norovirus-infected children with malnutrition in Indonesia
Background: Norovirus infection is the second most frequent viral agent causing gastroenteritis worldwide. Asymptomatic norovirus infection is prevalent in children and is associated with growth failure. Evaluating the intestinal inflammation of asymptomatic norovirus infection is pivotal to overcom...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-04-01
|
| Series: | Journal of Infection and Public Health |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187603412500036X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823856869799624704 |
|---|---|
| author | Anisa Lailatul Fitria Zayyin Dinana Aussie Tahta Maharani Alinda Syifa Fumizuki Faiz Nabila Mumtazya Dominikus Raditya Atmaka Laura Navika Yamani Juniastuti Maria Inge Lusida Soetjipto Septa Indra Puspikawati |
| author_facet | Anisa Lailatul Fitria Zayyin Dinana Aussie Tahta Maharani Alinda Syifa Fumizuki Faiz Nabila Mumtazya Dominikus Raditya Atmaka Laura Navika Yamani Juniastuti Maria Inge Lusida Soetjipto Septa Indra Puspikawati |
| author_sort | Anisa Lailatul Fitria |
| collection | DOAJ |
| description | Background: Norovirus infection is the second most frequent viral agent causing gastroenteritis worldwide. Asymptomatic norovirus infection is prevalent in children and is associated with growth failure. Evaluating the intestinal inflammation of asymptomatic norovirus infection is pivotal to overcoming malnutrition, especially in resource-limited settings. The current study aimed to compare the level of fecal neopterin and calprotectin, as the well-established biomarker for intestinal inflammation, in malnourished children with symptomatic and asymptomatic norovirus infections. Methods: In total 78 stool samples from children under five years old were analyzed in this comparative study. The 67 of the participants were malnourished children, with 24 had asymptomatic (ANV) and 18 had symptomatic norovirus infection (SNV), while 25 had no infections (Mal). We assigned 11 healthy children without any infection as control (Healthy). We assessed the fecal neopterin and calprotectin from the stools samples collected during viral diarrhea surveillance in Surabaya, East Java, Indonesia, using commercially ELISA kit from BT Lab (Zhejiang, China) for human neopterin and calprotectin. The statistical differences were tested using Kruskal-Wallis nonparametric test. Results: The level of fecal neopterin (mean ± SD, 15.46 ± 1.75 nmol/L and 15.12 ± 2.41 nmol/L; p-value: 0.744) and calprotectin (mean ± SD, 184.35 ± 35.09 ng/ml and 177.95 ± 49.55 ng/ml; p-value: 0.457) between ANV and SNV was comparable, also between Mal and Healthy groups. Meanwhile, the level of both biomarkers was significantly different in norovirus positive group and norovirus negative group (p-value < 0.000). Conclusions: Norovirus infection, regardless of the presence of symptoms, triggered intestinal inflammation, represented by the increased of fecal neopterin and calprotectin level. |
| format | Article |
| id | doaj-art-ef973966a0de423faacc4e45257e73e4 |
| institution | Kabale University |
| issn | 1876-0341 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Infection and Public Health |
| spelling | doaj-art-ef973966a0de423faacc4e45257e73e42025-02-12T05:30:56ZengElsevierJournal of Infection and Public Health1876-03412025-04-01184102687Level of fecal neopterin and calprotectin in asymptomatic and symptomatic norovirus-infected children with malnutrition in IndonesiaAnisa Lailatul Fitria0Zayyin Dinana1Aussie Tahta Maharani2Alinda Syifa Fumizuki3Faiz Nabila Mumtazya4Dominikus Raditya Atmaka5Laura Navika Yamani6 Juniastuti7Maria Inge Lusida8 Soetjipto9Septa Indra Puspikawati10Department of Nutrition, Faculty of Public Health, Universitas Airlangga, Indonesia; Viral Diarrhea Study Group, Institute of Tropical Disease, Universitas Airlangga, IndonesiaViral Diarrhea Study Group, Institute of Tropical Disease, Universitas Airlangga, IndonesiaViral Diarrhea Study Group, Institute of Tropical Disease, Universitas Airlangga, IndonesiaUndergraduate Program of Nutrition, Faculty of Public Health, Universitas Airlangga, IndonesiaUndergraduate Program of Nutrition, Faculty of Public Health, Universitas Airlangga, IndonesiaDepartment of Nutrition, Faculty of Public Health, Universitas Airlangga, Indonesia; Correspondence to: Department of Nutrition, Faculty of Public Health, Universitas Airlangga, Kampus C, Jl. Mulyorejo, Surabaya, East Java 60115, Indonesia.Viral Diarrhea Study Group, Institute of Tropical Disease, Universitas Airlangga, Indonesia; Department of Epidemiology, Faculty of Public Health, Universitas Airlangga, IndonesiaViral Diarrhea Study Group, Institute of Tropical Disease, Universitas Airlangga, Indonesia; Department of Microbiology, Faculty of Medicine, Universitas Airlangga, IndonesiaViral Diarrhea Study Group, Institute of Tropical Disease, Universitas Airlangga, Indonesia; Department of Microbiology, Faculty of Medicine, Universitas Airlangga, IndonesiaViral Diarrhea Study Group, Institute of Tropical Disease, Universitas Airlangga, Indonesia; Department of Biochemistry, Faculty of Medicine, Universitas Airlangga, Indonesia; Correspondence to: Department of Biochemistry, Faculty of Medicine, Universitas Airlangga, Jl. Mayjen Prof. Dr. Moestopo 47, Surabaya, East Java 68425, Indonesia.Faculty of Health Science, Medicine and Life Science, Universitas Airlangga, IndonesiaBackground: Norovirus infection is the second most frequent viral agent causing gastroenteritis worldwide. Asymptomatic norovirus infection is prevalent in children and is associated with growth failure. Evaluating the intestinal inflammation of asymptomatic norovirus infection is pivotal to overcoming malnutrition, especially in resource-limited settings. The current study aimed to compare the level of fecal neopterin and calprotectin, as the well-established biomarker for intestinal inflammation, in malnourished children with symptomatic and asymptomatic norovirus infections. Methods: In total 78 stool samples from children under five years old were analyzed in this comparative study. The 67 of the participants were malnourished children, with 24 had asymptomatic (ANV) and 18 had symptomatic norovirus infection (SNV), while 25 had no infections (Mal). We assigned 11 healthy children without any infection as control (Healthy). We assessed the fecal neopterin and calprotectin from the stools samples collected during viral diarrhea surveillance in Surabaya, East Java, Indonesia, using commercially ELISA kit from BT Lab (Zhejiang, China) for human neopterin and calprotectin. The statistical differences were tested using Kruskal-Wallis nonparametric test. Results: The level of fecal neopterin (mean ± SD, 15.46 ± 1.75 nmol/L and 15.12 ± 2.41 nmol/L; p-value: 0.744) and calprotectin (mean ± SD, 184.35 ± 35.09 ng/ml and 177.95 ± 49.55 ng/ml; p-value: 0.457) between ANV and SNV was comparable, also between Mal and Healthy groups. Meanwhile, the level of both biomarkers was significantly different in norovirus positive group and norovirus negative group (p-value < 0.000). Conclusions: Norovirus infection, regardless of the presence of symptoms, triggered intestinal inflammation, represented by the increased of fecal neopterin and calprotectin level.http://www.sciencedirect.com/science/article/pii/S187603412500036XNorovirusMalnutritionCalprotectinNeopterinIntestinal inflammation |
| spellingShingle | Anisa Lailatul Fitria Zayyin Dinana Aussie Tahta Maharani Alinda Syifa Fumizuki Faiz Nabila Mumtazya Dominikus Raditya Atmaka Laura Navika Yamani Juniastuti Maria Inge Lusida Soetjipto Septa Indra Puspikawati Level of fecal neopterin and calprotectin in asymptomatic and symptomatic norovirus-infected children with malnutrition in Indonesia Journal of Infection and Public Health Norovirus Malnutrition Calprotectin Neopterin Intestinal inflammation |
| title | Level of fecal neopterin and calprotectin in asymptomatic and symptomatic norovirus-infected children with malnutrition in Indonesia |
| title_full | Level of fecal neopterin and calprotectin in asymptomatic and symptomatic norovirus-infected children with malnutrition in Indonesia |
| title_fullStr | Level of fecal neopterin and calprotectin in asymptomatic and symptomatic norovirus-infected children with malnutrition in Indonesia |
| title_full_unstemmed | Level of fecal neopterin and calprotectin in asymptomatic and symptomatic norovirus-infected children with malnutrition in Indonesia |
| title_short | Level of fecal neopterin and calprotectin in asymptomatic and symptomatic norovirus-infected children with malnutrition in Indonesia |
| title_sort | level of fecal neopterin and calprotectin in asymptomatic and symptomatic norovirus infected children with malnutrition in indonesia |
| topic | Norovirus Malnutrition Calprotectin Neopterin Intestinal inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S187603412500036X |
| work_keys_str_mv | AT anisalailatulfitria leveloffecalneopterinandcalprotectininasymptomaticandsymptomaticnorovirusinfectedchildrenwithmalnutritioninindonesia AT zayyindinana leveloffecalneopterinandcalprotectininasymptomaticandsymptomaticnorovirusinfectedchildrenwithmalnutritioninindonesia AT aussietahtamaharani leveloffecalneopterinandcalprotectininasymptomaticandsymptomaticnorovirusinfectedchildrenwithmalnutritioninindonesia AT alindasyifafumizuki leveloffecalneopterinandcalprotectininasymptomaticandsymptomaticnorovirusinfectedchildrenwithmalnutritioninindonesia AT faiznabilamumtazya leveloffecalneopterinandcalprotectininasymptomaticandsymptomaticnorovirusinfectedchildrenwithmalnutritioninindonesia AT dominikusradityaatmaka leveloffecalneopterinandcalprotectininasymptomaticandsymptomaticnorovirusinfectedchildrenwithmalnutritioninindonesia AT lauranavikayamani leveloffecalneopterinandcalprotectininasymptomaticandsymptomaticnorovirusinfectedchildrenwithmalnutritioninindonesia AT juniastuti leveloffecalneopterinandcalprotectininasymptomaticandsymptomaticnorovirusinfectedchildrenwithmalnutritioninindonesia AT mariaingelusida leveloffecalneopterinandcalprotectininasymptomaticandsymptomaticnorovirusinfectedchildrenwithmalnutritioninindonesia AT soetjipto leveloffecalneopterinandcalprotectininasymptomaticandsymptomaticnorovirusinfectedchildrenwithmalnutritioninindonesia AT septaindrapuspikawati leveloffecalneopterinandcalprotectininasymptomaticandsymptomaticnorovirusinfectedchildrenwithmalnutritioninindonesia |