The Inhibitory Effect of Connective Tissue Growth Factor Antibody on Postoperative Fibrosis in a Rabbit Model of Trabeculectomy
Abstract Purpose: To compare the efficacy of subconjunctival injection of an anti-connective tissue growth factor antibody (anti-CTGF) versus mitomycin-C (MMC) and placebo in reducing scar formation in a rabbit model of trabeculectomy. Methods: A total of 14 rabbits were included. Nine rabbits under...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Knowledge E
2022-11-01
|
| Series: | Journal of Ophthalmic & Vision Research |
| Subjects: | |
| Online Access: | https://doi.org/10.18502/jovr.v17i4.12300 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841543729134960640 |
|---|---|
| author | Kiana Hassanpour Mozhgan Rezaei Kanavi Narsis Daftarian Azadeh Samaeili Fatemeh Suri Mohammad Pakravan Azadeh Doozandeh Sasha Afsar Aski Maryam Fakhri Afrooz Moghaddasi Hamid Ahmadieh Hamed Esfandiari |
| author_facet | Kiana Hassanpour Mozhgan Rezaei Kanavi Narsis Daftarian Azadeh Samaeili Fatemeh Suri Mohammad Pakravan Azadeh Doozandeh Sasha Afsar Aski Maryam Fakhri Afrooz Moghaddasi Hamid Ahmadieh Hamed Esfandiari |
| author_sort | Kiana Hassanpour |
| collection | DOAJ |
| description | Abstract Purpose: To compare the efficacy of subconjunctival injection of an anti-connective tissue growth factor antibody (anti-CTGF) versus mitomycin-C (MMC) and placebo in reducing scar formation in a rabbit model of trabeculectomy. Methods: A total of 14 rabbits were included. Nine rabbits underwent trabeculectomy with subconjunctival injections of either anti-CTGF antibody, MMC, or balanced salt solution (BSS), each administered in three eyes, before peritomy. The anti-CTGF group received a repeated dose of the antibody five days after surgery. All nine rabbits were euthanized on day 14; the globes were stained with hematoxylin & eosin, Masson's Trichrome, and immunohistochemistry for detecting alpha-smooth muscle (α-SMA) actin. RNA extraction was performed on five eyes of the remaining rabbits which included one eye without any surgery, one eye 5 hr after trabeculectomy without any injection, one eye five days after trabeculectomy without any injection, and two eyes five days after trabeculectomy with administration of MMC and BSS, respectively. Results: The mean bleb area in the anti-CTGF, MMC, and control groups was 3.8 ± 1.45, 5.9 ± 1.4, and 3.5 ± 1.9 mm2, respectively. Collagenous tissue was found to occupy the bleb area by 13.7%, 13.5%, and 18.5%, respectively. This ratio was significantly higher in the BSS group (P = 0.04). The expression of CTGF mRNA after 5 hr and five days in eyes undergoing trabeculectomy were significantly more pronounced as compared to the unoperated eye. The mean H-SCORE of α-SMA-immune reactive cells calculated as the grade of staining multiplied by the percentage of immune stained cells was 14.6, 10.22, and 140.58 in the anti-CTGF, MMC, and control groups, respectively. While the control eyes had a significantly higher score (Ps < 0.001), the anti-CTGF and MMC groups were comparable (P = 0.87). Conclusion: Based on the results of this animal study, the anti-CTGF antibody injection resulted in a significant reduction in collagenous tissue and myofibroblast cells after trabeculectomy. |
| format | Article |
| id | doaj-art-ef9530e9185b414094b5db071afe38c2 |
| institution | Kabale University |
| issn | 2008-2010 2008-322X |
| language | English |
| publishDate | 2022-11-01 |
| publisher | Knowledge E |
| record_format | Article |
| series | Journal of Ophthalmic & Vision Research |
| spelling | doaj-art-ef9530e9185b414094b5db071afe38c22025-01-13T07:14:26ZengKnowledge EJournal of Ophthalmic & Vision Research2008-20102008-322X2022-11-0117448649610.18502/jovr.v17i4.12300jovr.v17i4.12300The Inhibitory Effect of Connective Tissue Growth Factor Antibody on Postoperative Fibrosis in a Rabbit Model of TrabeculectomyKiana Hassanpour0Mozhgan Rezaei Kanavi1Narsis Daftarian2Azadeh Samaeili3Fatemeh Suri4Mohammad Pakravan5Azadeh Doozandeh6Sasha Afsar Aski7Maryam Fakhri8Afrooz Moghaddasi9Hamid Ahmadieh10Hamed Esfandiari11 Ophthalmic Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ocular Tissue Engineering Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ocular Tissue Engineering Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ophthalmic Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ophthalmic Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ophthalmic Epidemiology Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ophthalmic Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ocular Tissue Engineering Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ophthalmic Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ophthalmic Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ophthalmic Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran Department of ophthalmology, Olmsted Medical Center, Rochester, MN, USAAbstract Purpose: To compare the efficacy of subconjunctival injection of an anti-connective tissue growth factor antibody (anti-CTGF) versus mitomycin-C (MMC) and placebo in reducing scar formation in a rabbit model of trabeculectomy. Methods: A total of 14 rabbits were included. Nine rabbits underwent trabeculectomy with subconjunctival injections of either anti-CTGF antibody, MMC, or balanced salt solution (BSS), each administered in three eyes, before peritomy. The anti-CTGF group received a repeated dose of the antibody five days after surgery. All nine rabbits were euthanized on day 14; the globes were stained with hematoxylin & eosin, Masson's Trichrome, and immunohistochemistry for detecting alpha-smooth muscle (α-SMA) actin. RNA extraction was performed on five eyes of the remaining rabbits which included one eye without any surgery, one eye 5 hr after trabeculectomy without any injection, one eye five days after trabeculectomy without any injection, and two eyes five days after trabeculectomy with administration of MMC and BSS, respectively. Results: The mean bleb area in the anti-CTGF, MMC, and control groups was 3.8 ± 1.45, 5.9 ± 1.4, and 3.5 ± 1.9 mm2, respectively. Collagenous tissue was found to occupy the bleb area by 13.7%, 13.5%, and 18.5%, respectively. This ratio was significantly higher in the BSS group (P = 0.04). The expression of CTGF mRNA after 5 hr and five days in eyes undergoing trabeculectomy were significantly more pronounced as compared to the unoperated eye. The mean H-SCORE of α-SMA-immune reactive cells calculated as the grade of staining multiplied by the percentage of immune stained cells was 14.6, 10.22, and 140.58 in the anti-CTGF, MMC, and control groups, respectively. While the control eyes had a significantly higher score (Ps < 0.001), the anti-CTGF and MMC groups were comparable (P = 0.87). Conclusion: Based on the results of this animal study, the anti-CTGF antibody injection resulted in a significant reduction in collagenous tissue and myofibroblast cells after trabeculectomy.https://doi.org/10.18502/jovr.v17i4.12300anti-connective tissue growth factorctgfmitomycin-ctrabeculectomy |
| spellingShingle | Kiana Hassanpour Mozhgan Rezaei Kanavi Narsis Daftarian Azadeh Samaeili Fatemeh Suri Mohammad Pakravan Azadeh Doozandeh Sasha Afsar Aski Maryam Fakhri Afrooz Moghaddasi Hamid Ahmadieh Hamed Esfandiari The Inhibitory Effect of Connective Tissue Growth Factor Antibody on Postoperative Fibrosis in a Rabbit Model of Trabeculectomy Journal of Ophthalmic & Vision Research anti-connective tissue growth factor ctgf mitomycin-c trabeculectomy |
| title | The Inhibitory Effect of Connective Tissue Growth Factor Antibody on Postoperative Fibrosis in a Rabbit Model of Trabeculectomy |
| title_full | The Inhibitory Effect of Connective Tissue Growth Factor Antibody on Postoperative Fibrosis in a Rabbit Model of Trabeculectomy |
| title_fullStr | The Inhibitory Effect of Connective Tissue Growth Factor Antibody on Postoperative Fibrosis in a Rabbit Model of Trabeculectomy |
| title_full_unstemmed | The Inhibitory Effect of Connective Tissue Growth Factor Antibody on Postoperative Fibrosis in a Rabbit Model of Trabeculectomy |
| title_short | The Inhibitory Effect of Connective Tissue Growth Factor Antibody on Postoperative Fibrosis in a Rabbit Model of Trabeculectomy |
| title_sort | inhibitory effect of connective tissue growth factor antibody on postoperative fibrosis in a rabbit model of trabeculectomy |
| topic | anti-connective tissue growth factor ctgf mitomycin-c trabeculectomy |
| url | https://doi.org/10.18502/jovr.v17i4.12300 |
| work_keys_str_mv | AT kianahassanpour theinhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT mozhganrezaeikanavi theinhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT narsisdaftarian theinhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT azadehsamaeili theinhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT fatemehsuri theinhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT mohammadpakravan theinhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT azadehdoozandeh theinhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT sashaafsaraski theinhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT maryamfakhri theinhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT afroozmoghaddasi theinhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT hamidahmadieh theinhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT hamedesfandiari theinhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT kianahassanpour inhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT mozhganrezaeikanavi inhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT narsisdaftarian inhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT azadehsamaeili inhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT fatemehsuri inhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT mohammadpakravan inhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT azadehdoozandeh inhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT sashaafsaraski inhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT maryamfakhri inhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT afroozmoghaddasi inhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT hamidahmadieh inhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy AT hamedesfandiari inhibitoryeffectofconnectivetissuegrowthfactorantibodyonpostoperativefibrosisinarabbitmodeloftrabeculectomy |