Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes.
Although laccase has been recognized as a wonder molecule and green enzyme, the use of low yielding fungal strains, poor production, purification, and low enzyme kinetics have hampered its large-scale application. Thus,this study aims to select high yielding fungal strains and optimize the productio...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0229968 |
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| author | R Z Sayyed H M Bhamare Sapna Najat Marraiki Abdallah M Elgorban Asad Syed Hesham Ali El-Enshasy Daniel J Dailin |
| author_facet | R Z Sayyed H M Bhamare Sapna Najat Marraiki Abdallah M Elgorban Asad Syed Hesham Ali El-Enshasy Daniel J Dailin |
| author_sort | R Z Sayyed |
| collection | DOAJ |
| description | Although laccase has been recognized as a wonder molecule and green enzyme, the use of low yielding fungal strains, poor production, purification, and low enzyme kinetics have hampered its large-scale application. Thus,this study aims to select high yielding fungal strains and optimize the production, purification, and kinetics of laccase of Aspergillus sp. HB_RZ4. The results obtained indicated that Aspergillus sp. HB_RZ4 produced a significantly large amount of laccase under meso-acidophilic shaking conditions in a medium containing glucose and yeast extract. A 25 μM CuSO4 was observed to enhance the enzyme yield. The enzyme was best purified on a Sephadex G-100 column. The purified enzyme resembled laccase of A. flavus. The kinetics of the purified enzyme revealed high substrate specificity and good velocity of reaction,using ABTS as a substrate. The enzyme was observed to be stable over various pH values and temperatures. The peptide structure of the purified enzyme was found to resemble laccase of A. kawachii IFO 4308. The fungus was observed to decolorize various dyes independent of the requirement of a laccase mediator system.Aspergillus sp. HB_RZ4 was observed to be a potent natural producer of laccase, and it decolorized the dyes even in the absence of a laccase mediator system. Thus, it can be used for bioremediation of effluent that contains non-textile dyes. |
| format | Article |
| id | doaj-art-ef8efbbcb9a34a44a79dfddaf263edd3 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-ef8efbbcb9a34a44a79dfddaf263edd32025-08-20T03:13:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01156e022996810.1371/journal.pone.0229968Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes.R Z SayyedH M BhamareSapnaNajat MarraikiAbdallah M ElgorbanAsad SyedHesham Ali El-EnshasyDaniel J DailinAlthough laccase has been recognized as a wonder molecule and green enzyme, the use of low yielding fungal strains, poor production, purification, and low enzyme kinetics have hampered its large-scale application. Thus,this study aims to select high yielding fungal strains and optimize the production, purification, and kinetics of laccase of Aspergillus sp. HB_RZ4. The results obtained indicated that Aspergillus sp. HB_RZ4 produced a significantly large amount of laccase under meso-acidophilic shaking conditions in a medium containing glucose and yeast extract. A 25 μM CuSO4 was observed to enhance the enzyme yield. The enzyme was best purified on a Sephadex G-100 column. The purified enzyme resembled laccase of A. flavus. The kinetics of the purified enzyme revealed high substrate specificity and good velocity of reaction,using ABTS as a substrate. The enzyme was observed to be stable over various pH values and temperatures. The peptide structure of the purified enzyme was found to resemble laccase of A. kawachii IFO 4308. The fungus was observed to decolorize various dyes independent of the requirement of a laccase mediator system.Aspergillus sp. HB_RZ4 was observed to be a potent natural producer of laccase, and it decolorized the dyes even in the absence of a laccase mediator system. Thus, it can be used for bioremediation of effluent that contains non-textile dyes.https://doi.org/10.1371/journal.pone.0229968 |
| spellingShingle | R Z Sayyed H M Bhamare Sapna Najat Marraiki Abdallah M Elgorban Asad Syed Hesham Ali El-Enshasy Daniel J Dailin Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes. PLoS ONE |
| title | Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes. |
| title_full | Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes. |
| title_fullStr | Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes. |
| title_full_unstemmed | Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes. |
| title_short | Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes. |
| title_sort | tree bark scrape fungus a potential source of laccase for application in bioremediation of non textile dyes |
| url | https://doi.org/10.1371/journal.pone.0229968 |
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