Effects of Various Decellularization Methods for the Development of Decellularized Extracellular Matrix from Tilapia (Oreochromis niloticus) Viscera
Tilapia, a widely farmed aquaculture fish, produces substantial waste, including viscera that contain extracellular matrix (ECM) utilized as a biomaterial for tissue regeneration applications. Extracting ECM from viscera requires a specific decellularization method, as no standardized protocol exist...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2024/6148496 |
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| author | Jemwel Aron Ronald Bual Johnel Alimasag Fernan Arellano Lean Baclayon Zesreal Cain Bantilan Gladine Lumancas Michael John Nisperos Marionilo Labares Kit Dominick Don Valle Hernando Bacosa |
| author_facet | Jemwel Aron Ronald Bual Johnel Alimasag Fernan Arellano Lean Baclayon Zesreal Cain Bantilan Gladine Lumancas Michael John Nisperos Marionilo Labares Kit Dominick Don Valle Hernando Bacosa |
| author_sort | Jemwel Aron |
| collection | DOAJ |
| description | Tilapia, a widely farmed aquaculture fish, produces substantial waste, including viscera that contain extracellular matrix (ECM) utilized as a biomaterial for tissue regeneration applications. Extracting ECM from viscera requires a specific decellularization method, as no standardized protocol exists. This study performed three decellularization methods: sonication, orbital shaking at room temperature, and agitation at 4°C, using SDS and TX100 at concentrations of 0.1% and 0.3%. The effectiveness of each method was assessed through H&E staining, dsDNA quantification, and SEM imaging to verify cellular content removal and ECM structure preservation. Additional analyses, including ATR-FTIR, SDS-PAGE, protein quantification, HPLC, and detergent residue tests, were performed to examine functional groups, collagen composition, protein content, amino acid profiles, and detergent residues in the decellularized samples. The results of H&E staining showed a significant reduction in cellular components in all samples, which was confirmed through DNA quantification. Sonication with 0.3% SDS achieved the highest DNA removal rate (96.5 ± 1.1%), while SEM images revealed that agitation at 4°C with 0.3% TX100 better preserved ECM structure. Collagen was present in all samples, as confirmed by ATR-FTIR analysis, which revealed pronounced spectral peaks in the amide I, II, III, A, and B regions. Samples treated with agitation at 4°C using 0.1% SDS exhibited the highest protein content (875 ± 15 µg/mg), whereas those treated with TX100 had lower detergent residue. Overall, the decellularization methods effectively reduced DNA content while preserving ECM structure and components, highlighting the potential of tilapia viscera as bioscaffolds and offering insights into utilizing fish waste for high-value products. |
| format | Article |
| id | doaj-art-ef859371f16243b7bd22de07e7e96f09 |
| institution | DOAJ |
| issn | 1687-8795 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-ef859371f16243b7bd22de07e7e96f092025-08-20T03:21:13ZengWileyInternational Journal of Biomaterials1687-87952024-01-01202410.1155/2024/6148496Effects of Various Decellularization Methods for the Development of Decellularized Extracellular Matrix from Tilapia (Oreochromis niloticus) VisceraJemwel Aron0Ronald Bual1Johnel Alimasag2Fernan Arellano3Lean Baclayon4Zesreal Cain Bantilan5Gladine Lumancas6Michael John Nisperos7Marionilo Labares8Kit Dominick Don Valle9Hernando Bacosa10Environmental Science Graduate Program-Department of Biological SciencesCenter for Sustainable PolymersCenter for Sustainable PolymersEnvironmental Science Graduate Program-Department of Biological SciencesEnvironmental Science Graduate Program-Department of Biological SciencesCenter for Sustainable PolymersEnvironmental Science Graduate Program-Department of Biological SciencesEnvironmental Science Graduate Program-Department of Biological SciencesCenter for Sustainable PolymersCenter for Sustainable PolymersEnvironmental Science Graduate Program-Department of Biological SciencesTilapia, a widely farmed aquaculture fish, produces substantial waste, including viscera that contain extracellular matrix (ECM) utilized as a biomaterial for tissue regeneration applications. Extracting ECM from viscera requires a specific decellularization method, as no standardized protocol exists. This study performed three decellularization methods: sonication, orbital shaking at room temperature, and agitation at 4°C, using SDS and TX100 at concentrations of 0.1% and 0.3%. The effectiveness of each method was assessed through H&E staining, dsDNA quantification, and SEM imaging to verify cellular content removal and ECM structure preservation. Additional analyses, including ATR-FTIR, SDS-PAGE, protein quantification, HPLC, and detergent residue tests, were performed to examine functional groups, collagen composition, protein content, amino acid profiles, and detergent residues in the decellularized samples. The results of H&E staining showed a significant reduction in cellular components in all samples, which was confirmed through DNA quantification. Sonication with 0.3% SDS achieved the highest DNA removal rate (96.5 ± 1.1%), while SEM images revealed that agitation at 4°C with 0.3% TX100 better preserved ECM structure. Collagen was present in all samples, as confirmed by ATR-FTIR analysis, which revealed pronounced spectral peaks in the amide I, II, III, A, and B regions. Samples treated with agitation at 4°C using 0.1% SDS exhibited the highest protein content (875 ± 15 µg/mg), whereas those treated with TX100 had lower detergent residue. Overall, the decellularization methods effectively reduced DNA content while preserving ECM structure and components, highlighting the potential of tilapia viscera as bioscaffolds and offering insights into utilizing fish waste for high-value products.http://dx.doi.org/10.1155/2024/6148496 |
| spellingShingle | Jemwel Aron Ronald Bual Johnel Alimasag Fernan Arellano Lean Baclayon Zesreal Cain Bantilan Gladine Lumancas Michael John Nisperos Marionilo Labares Kit Dominick Don Valle Hernando Bacosa Effects of Various Decellularization Methods for the Development of Decellularized Extracellular Matrix from Tilapia (Oreochromis niloticus) Viscera International Journal of Biomaterials |
| title | Effects of Various Decellularization Methods for the Development of Decellularized Extracellular Matrix from Tilapia (Oreochromis niloticus) Viscera |
| title_full | Effects of Various Decellularization Methods for the Development of Decellularized Extracellular Matrix from Tilapia (Oreochromis niloticus) Viscera |
| title_fullStr | Effects of Various Decellularization Methods for the Development of Decellularized Extracellular Matrix from Tilapia (Oreochromis niloticus) Viscera |
| title_full_unstemmed | Effects of Various Decellularization Methods for the Development of Decellularized Extracellular Matrix from Tilapia (Oreochromis niloticus) Viscera |
| title_short | Effects of Various Decellularization Methods for the Development of Decellularized Extracellular Matrix from Tilapia (Oreochromis niloticus) Viscera |
| title_sort | effects of various decellularization methods for the development of decellularized extracellular matrix from tilapia oreochromis niloticus viscera |
| url | http://dx.doi.org/10.1155/2024/6148496 |
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