Viability of mesenchymal stem cells of adipose tissue from human liposuction
Introduction: Lipografting is an alternative with important applicability for breast reconstruction and/or corrections of asymmetries resulting from cancer treatment. This technique consists of autologous fat transfer, whose stroma contains stem cells derived from adipose tissue that can differentia...
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| Format: | Article |
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Thieme Revinter Publicações Ltda.
2021-03-01
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| Series: | Revista Brasileira de Cirurgia Plástica |
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| Online Access: | http://www.rbcp.org.br/export-pdf/2881/en_v36n1a03.pdf |
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| author | Marcelo Moreno Juliana Cristina Schmidt Candice Detoni Gazzoni Letícia Dal-Magro Beatriz da Silva Rosa Bonadiman Greicy Cristine Kosvoski Filomena Marafon Margarete Dulce Bagatini |
| author_facet | Marcelo Moreno Juliana Cristina Schmidt Candice Detoni Gazzoni Letícia Dal-Magro Beatriz da Silva Rosa Bonadiman Greicy Cristine Kosvoski Filomena Marafon Margarete Dulce Bagatini |
| author_sort | Marcelo Moreno |
| collection | DOAJ |
| description | Introduction: Lipografting is an alternative with important applicability for breast reconstruction and/or corrections of asymmetries resulting from cancer treatment. This technique consists of autologous fat transfer, whose stroma contains stem cells derived from adipose tissue that can differentiate itself throughout the mesodermal lineage. For adipose tissue preparation, Coleman-based centrifugation of syringe-aspirated material at 3000 revolutions per minute (rpm) for 3 minutes. However, studies question whether lower centrifugation speeds could be less harmful to cell viability.
Methods: An experimental study was conducted to evaluate the adipose cells of six patients; from 60mL of liposuction of each one. The sample collected was fractionated into four tubes and submitted to different protocols, decanting and centrifugation at speeds 500, 1000, and 3000rpm for 3 minutes. Afterward, the samples were processed with collagenase IA for 30 min, submitted to cell culture for 24 hours, and a cell viability analysis. The results were tabulated and analyzed by the ANOVA test using the Graphpad Prism 6.0® and SAS®.
Results: Cell viability was higher in the cell sample centrifuged at 3000rpm and lower in the decanted sample. Giemsa staining indicated maintenance of cell morphology on the samples.
Conclusion: Centrifuged cells at a speed of 3000rpm showed higher cell viability. Centrifugation was effective in compacting tissue and eliminating unwanted waste (blood and residual oil). |
| format | Article |
| id | doaj-art-ef845b582c464296a2a6981a4a04f952 |
| institution | DOAJ |
| issn | 1983-5175 2177-1235 |
| language | English |
| publishDate | 2021-03-01 |
| publisher | Thieme Revinter Publicações Ltda. |
| record_format | Article |
| series | Revista Brasileira de Cirurgia Plástica |
| spelling | doaj-art-ef845b582c464296a2a6981a4a04f9522025-08-20T03:22:21ZengThieme Revinter Publicações Ltda.Revista Brasileira de Cirurgia Plástica1983-51752177-12352021-03-013601091410.5935/2177-1235.2021RBCP0003Viability of mesenchymal stem cells of adipose tissue from human liposuctionMarcelo Moreno0Juliana Cristina Schmidt1Candice Detoni Gazzoni2Letícia Dal-Magro3Beatriz da Silva Rosa Bonadiman4Greicy Cristine Kosvoski5Filomena Marafon6Margarete Dulce Bagatini7Federal University of Fronteira Sul, Chapecó, SC, BrazilCommunity University of the Region of Chapecó, Medicine Course, Chapecó, SC, BrazilCommunity University of the Region of Chapecó, Medicine Course, Chapecó, SC, BrazilCommunity University of the Region of Chapecó, Medicine Course, Chapecó, SC, BrazilFederal University of Fronteira Sul, Chapecó, SC, BrazilCommunity University of the Region of Chapecó, Medicine Course, Chapecó, SC, BrazilFederal University of Fronteira Sul, Chapecó, SC, BrazilFederal University of Fronteira Sul, Chapecó, SC, BrazilIntroduction: Lipografting is an alternative with important applicability for breast reconstruction and/or corrections of asymmetries resulting from cancer treatment. This technique consists of autologous fat transfer, whose stroma contains stem cells derived from adipose tissue that can differentiate itself throughout the mesodermal lineage. For adipose tissue preparation, Coleman-based centrifugation of syringe-aspirated material at 3000 revolutions per minute (rpm) for 3 minutes. However, studies question whether lower centrifugation speeds could be less harmful to cell viability. Methods: An experimental study was conducted to evaluate the adipose cells of six patients; from 60mL of liposuction of each one. The sample collected was fractionated into four tubes and submitted to different protocols, decanting and centrifugation at speeds 500, 1000, and 3000rpm for 3 minutes. Afterward, the samples were processed with collagenase IA for 30 min, submitted to cell culture for 24 hours, and a cell viability analysis. The results were tabulated and analyzed by the ANOVA test using the Graphpad Prism 6.0® and SAS®. Results: Cell viability was higher in the cell sample centrifuged at 3000rpm and lower in the decanted sample. Giemsa staining indicated maintenance of cell morphology on the samples. Conclusion: Centrifuged cells at a speed of 3000rpm showed higher cell viability. Centrifugation was effective in compacting tissue and eliminating unwanted waste (blood and residual oil).http://www.rbcp.org.br/export-pdf/2881/en_v36n1a03.pdfstem cellscentrifugelipectomymammoplastyadipocytes |
| spellingShingle | Marcelo Moreno Juliana Cristina Schmidt Candice Detoni Gazzoni Letícia Dal-Magro Beatriz da Silva Rosa Bonadiman Greicy Cristine Kosvoski Filomena Marafon Margarete Dulce Bagatini Viability of mesenchymal stem cells of adipose tissue from human liposuction Revista Brasileira de Cirurgia Plástica stem cells centrifuge lipectomy mammoplasty adipocytes |
| title | Viability of mesenchymal stem cells of adipose tissue from human liposuction |
| title_full | Viability of mesenchymal stem cells of adipose tissue from human liposuction |
| title_fullStr | Viability of mesenchymal stem cells of adipose tissue from human liposuction |
| title_full_unstemmed | Viability of mesenchymal stem cells of adipose tissue from human liposuction |
| title_short | Viability of mesenchymal stem cells of adipose tissue from human liposuction |
| title_sort | viability of mesenchymal stem cells of adipose tissue from human liposuction |
| topic | stem cells centrifuge lipectomy mammoplasty adipocytes |
| url | http://www.rbcp.org.br/export-pdf/2881/en_v36n1a03.pdf |
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