Development of an iPSC-derived immunocompetent skin model for identification of skin sensitizing substances
The development of immunocompetent skin models marks a significant advancement in in vitro methods for detecting skin sensitizers while adhering to the 3R principles, which aim to reduce, refine, and replace animal testing. This study introduces for the first time an advanced immunocompetent skin mo...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2025-05-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/20417314251336296 |
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| _version_ | 1849315028098875392 |
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| author | Marla Dubau Tarada Tripetchr Lava Mahmoud Fabian Schumacher Burkhard Kleuser |
| author_facet | Marla Dubau Tarada Tripetchr Lava Mahmoud Fabian Schumacher Burkhard Kleuser |
| author_sort | Marla Dubau |
| collection | DOAJ |
| description | The development of immunocompetent skin models marks a significant advancement in in vitro methods for detecting skin sensitizers while adhering to the 3R principles, which aim to reduce, refine, and replace animal testing. This study introduces for the first time an advanced immunocompetent skin model constructed entirely from induced pluripotent stem cell (iPSC)-derived cell types, including fibroblasts (iPSC-FB), keratinocytes (iPSC-KC), and fully integrated dendritic cells (iPSC-DC). To evaluate the skin model’s capacity, the model was treated topically with a range of well-characterized skin sensitizers varying in potency. The results indicate that the iPSC-derived immunocompetent skin model successfully replicates the physiological responses of human skin, offering a robust and reliable alternative to animal models for skin sensitization testing, allowing detection of extreme and even weak sensitizers. By addressing critical aspects of immune activation and cytokine signaling, this model provides an ethical, comprehensive tool for regulatory toxicology and dermatological research. |
| format | Article |
| id | doaj-art-ef83fee0ac4841fa96710a2fbd020985 |
| institution | Kabale University |
| issn | 2041-7314 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj-art-ef83fee0ac4841fa96710a2fbd0209852025-08-20T03:52:15ZengSAGE PublishingJournal of Tissue Engineering2041-73142025-05-011610.1177/20417314251336296Development of an iPSC-derived immunocompetent skin model for identification of skin sensitizing substancesMarla Dubau0Tarada Tripetchr1Lava Mahmoud2Fabian Schumacher3Burkhard Kleuser4Freie Universität Berlin, Department of Pharmacology and Toxicology, Institute of Pharmacy, Berlin, GermanyFreie Universität Berlin, Department of Pharmacology and Toxicology, Institute of Pharmacy, Berlin, GermanyFreie Universität Berlin, Department of Pharmacology and Toxicology, Institute of Pharmacy, Berlin, GermanyFreie Universität Berlin, Department of Pharmacology and Toxicology, Institute of Pharmacy, Berlin, GermanyFreie Universität Berlin, Department of Pharmacology and Toxicology, Institute of Pharmacy, Berlin, GermanyThe development of immunocompetent skin models marks a significant advancement in in vitro methods for detecting skin sensitizers while adhering to the 3R principles, which aim to reduce, refine, and replace animal testing. This study introduces for the first time an advanced immunocompetent skin model constructed entirely from induced pluripotent stem cell (iPSC)-derived cell types, including fibroblasts (iPSC-FB), keratinocytes (iPSC-KC), and fully integrated dendritic cells (iPSC-DC). To evaluate the skin model’s capacity, the model was treated topically with a range of well-characterized skin sensitizers varying in potency. The results indicate that the iPSC-derived immunocompetent skin model successfully replicates the physiological responses of human skin, offering a robust and reliable alternative to animal models for skin sensitization testing, allowing detection of extreme and even weak sensitizers. By addressing critical aspects of immune activation and cytokine signaling, this model provides an ethical, comprehensive tool for regulatory toxicology and dermatological research.https://doi.org/10.1177/20417314251336296 |
| spellingShingle | Marla Dubau Tarada Tripetchr Lava Mahmoud Fabian Schumacher Burkhard Kleuser Development of an iPSC-derived immunocompetent skin model for identification of skin sensitizing substances Journal of Tissue Engineering |
| title | Development of an iPSC-derived immunocompetent skin model for identification of skin sensitizing substances |
| title_full | Development of an iPSC-derived immunocompetent skin model for identification of skin sensitizing substances |
| title_fullStr | Development of an iPSC-derived immunocompetent skin model for identification of skin sensitizing substances |
| title_full_unstemmed | Development of an iPSC-derived immunocompetent skin model for identification of skin sensitizing substances |
| title_short | Development of an iPSC-derived immunocompetent skin model for identification of skin sensitizing substances |
| title_sort | development of an ipsc derived immunocompetent skin model for identification of skin sensitizing substances |
| url | https://doi.org/10.1177/20417314251336296 |
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