Biochemical and Kinetic Study for the partially purified Lecithin: Cholesterol acyltransferase from serum cardiovascular disease
The study includes partial purification of Lecithin: Cholesterol acyltransferase (LCAT) from the blood serum of a person suffering from atherosclerosis. Several techniques were applied including ammonium sulphate precipitation, dialysis, ion exchange chromatography and electrophoresis. It was found...
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| Format: | Article |
| Language: | English |
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Tikrit University
2020-03-01
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| Series: | Tikrit Journal of Pure Science |
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| Online Access: | https://tjpsj.org/index.php/tjps/article/view/233 |
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| author | Amel Taha Yassein Al-Juraisy Ameera Aziz Mahmood Al-Juraisy Nadia Ahmed saleh |
| author_facet | Amel Taha Yassein Al-Juraisy Ameera Aziz Mahmood Al-Juraisy Nadia Ahmed saleh |
| author_sort | Amel Taha Yassein Al-Juraisy |
| collection | DOAJ |
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The study includes partial purification of Lecithin: Cholesterol acyltransferase (LCAT) from the blood serum of a person suffering from atherosclerosis. Several techniques were applied including ammonium sulphate precipitation, dialysis, ion exchange chromatography and electrophoresis. It was found that LCAT has one isoenzyme and the highest activity was (1073.46 × 10-3) unit/ml and a molecular weight of 62 KDa. The study also deals with characterizing of LCAT. It was found that the optimum pH and temp. of the enzyme were 7 and 35ºC.
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| format | Article |
| id | doaj-art-ef81370fda364958bfb2fee44746f560 |
| institution | Kabale University |
| issn | 1813-1662 2415-1726 |
| language | English |
| publishDate | 2020-03-01 |
| publisher | Tikrit University |
| record_format | Article |
| series | Tikrit Journal of Pure Science |
| spelling | doaj-art-ef81370fda364958bfb2fee44746f5602025-08-20T03:29:14ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262020-03-0125210.25130/tjps.v25i2.233Biochemical and Kinetic Study for the partially purified Lecithin: Cholesterol acyltransferase from serum cardiovascular diseaseAmel Taha Yassein Al-JuraisyAmeera Aziz Mahmood Al-JuraisyNadia Ahmed saleh The study includes partial purification of Lecithin: Cholesterol acyltransferase (LCAT) from the blood serum of a person suffering from atherosclerosis. Several techniques were applied including ammonium sulphate precipitation, dialysis, ion exchange chromatography and electrophoresis. It was found that LCAT has one isoenzyme and the highest activity was (1073.46 × 10-3) unit/ml and a molecular weight of 62 KDa. The study also deals with characterizing of LCAT. It was found that the optimum pH and temp. of the enzyme were 7 and 35ºC. https://tjpsj.org/index.php/tjps/article/view/233SeparationEnzymeLecithinCholesterol acyltransferase (LCAT) |
| spellingShingle | Amel Taha Yassein Al-Juraisy Ameera Aziz Mahmood Al-Juraisy Nadia Ahmed saleh Biochemical and Kinetic Study for the partially purified Lecithin: Cholesterol acyltransferase from serum cardiovascular disease Tikrit Journal of Pure Science Separation Enzyme Lecithin Cholesterol acyltransferase (LCAT) |
| title | Biochemical and Kinetic Study for the partially purified Lecithin: Cholesterol acyltransferase from serum cardiovascular disease |
| title_full | Biochemical and Kinetic Study for the partially purified Lecithin: Cholesterol acyltransferase from serum cardiovascular disease |
| title_fullStr | Biochemical and Kinetic Study for the partially purified Lecithin: Cholesterol acyltransferase from serum cardiovascular disease |
| title_full_unstemmed | Biochemical and Kinetic Study for the partially purified Lecithin: Cholesterol acyltransferase from serum cardiovascular disease |
| title_short | Biochemical and Kinetic Study for the partially purified Lecithin: Cholesterol acyltransferase from serum cardiovascular disease |
| title_sort | biochemical and kinetic study for the partially purified lecithin cholesterol acyltransferase from serum cardiovascular disease |
| topic | Separation Enzyme Lecithin Cholesterol acyltransferase (LCAT) |
| url | https://tjpsj.org/index.php/tjps/article/view/233 |
| work_keys_str_mv | AT ameltahayasseinaljuraisy biochemicalandkineticstudyforthepartiallypurifiedlecithincholesterolacyltransferasefromserumcardiovasculardisease AT ameeraazizmahmoodaljuraisy biochemicalandkineticstudyforthepartiallypurifiedlecithincholesterolacyltransferasefromserumcardiovasculardisease AT nadiaahmedsaleh biochemicalandkineticstudyforthepartiallypurifiedlecithincholesterolacyltransferasefromserumcardiovasculardisease |