Immobilization of Lipase on Agarose Beads for Enzymatic Hydrolsis/Transesterification of Castor Oil
Lipase was immobilized onto agarose beads and used for enzyme-catalyzed hydrolysis/transesterification of castor oil at room temperature without surfactants and salts. The immobilization yield was evaluated by reacting p-nitrophenyl butyrate and immobilized lipase. The reaction was performed in a 25...
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Sakarya University
2024-02-01
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| Series: | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/3402331 |
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| author | Kübra Karakaya Semra Yılmazer Keskin |
| author_facet | Kübra Karakaya Semra Yılmazer Keskin |
| author_sort | Kübra Karakaya |
| collection | DOAJ |
| description | Lipase was immobilized onto agarose beads and used for enzyme-catalyzed hydrolysis/transesterification of castor oil at room temperature without surfactants and salts. The immobilization yield was evaluated by reacting p-nitrophenyl butyrate and immobilized lipase. The reaction was performed in a 25 mL phosphate buffer medium (pH 7.0) and incubated for 2 hours. The absorbance of the formed p-nitrophenol was measured at 346 nm. The highest immobilization rate was obtained using 5.0 mg lipase with 2.5 mL glyoxal agarose beads. The reaction was performed in 25.0 mL sodium bicarbonate buffer pH 10 for 12 h. The hydrolysis/transesterification of castor oil was performed in phosphate buffer (pH 7) – ethanol medium. The main products were ricinoleic acid and ethyl ricinoleate. The highest hydrolysis/transesterification yield was 87% using 1.0 g castor oil and 2.5 mL of lipase immobilized agarose beads in the phosphate buffer (pH 7): ethanol (1: 9, V: V) at 72 hours. The reaction products were analyzed using a Fourier Transform Infrared spectrometer (FTIR) and Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC). |
| format | Article |
| id | doaj-art-ef7308f6c34047be9d3daed16f987e03 |
| institution | OA Journals |
| issn | 2147-835X |
| language | English |
| publishDate | 2024-02-01 |
| publisher | Sakarya University |
| record_format | Article |
| series | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
| spelling | doaj-art-ef7308f6c34047be9d3daed16f987e032025-08-20T02:31:43ZengSakarya UniversitySakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi2147-835X2024-02-01281515710.16984/saufenbilder.135902128Immobilization of Lipase on Agarose Beads for Enzymatic Hydrolsis/Transesterification of Castor OilKübra Karakaya0https://orcid.org/0009-0006-0608-9837Semra Yılmazer Keskin1https://orcid.org/0000-0002-9467-3171SAKARYA ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ, KİMYA (YL) (TEZLİ)SAKARYA ÜNİVERSİTESİLipase was immobilized onto agarose beads and used for enzyme-catalyzed hydrolysis/transesterification of castor oil at room temperature without surfactants and salts. The immobilization yield was evaluated by reacting p-nitrophenyl butyrate and immobilized lipase. The reaction was performed in a 25 mL phosphate buffer medium (pH 7.0) and incubated for 2 hours. The absorbance of the formed p-nitrophenol was measured at 346 nm. The highest immobilization rate was obtained using 5.0 mg lipase with 2.5 mL glyoxal agarose beads. The reaction was performed in 25.0 mL sodium bicarbonate buffer pH 10 for 12 h. The hydrolysis/transesterification of castor oil was performed in phosphate buffer (pH 7) – ethanol medium. The main products were ricinoleic acid and ethyl ricinoleate. The highest hydrolysis/transesterification yield was 87% using 1.0 g castor oil and 2.5 mL of lipase immobilized agarose beads in the phosphate buffer (pH 7): ethanol (1: 9, V: V) at 72 hours. The reaction products were analyzed using a Fourier Transform Infrared spectrometer (FTIR) and Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC).https://dergipark.org.tr/tr/download/article-file/3402331agarose beadsbiocatalysiscastor oillipasericinoleic acid |
| spellingShingle | Kübra Karakaya Semra Yılmazer Keskin Immobilization of Lipase on Agarose Beads for Enzymatic Hydrolsis/Transesterification of Castor Oil Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi agarose beads biocatalysis castor oil lipase ricinoleic acid |
| title | Immobilization of Lipase on Agarose Beads for Enzymatic Hydrolsis/Transesterification of Castor Oil |
| title_full | Immobilization of Lipase on Agarose Beads for Enzymatic Hydrolsis/Transesterification of Castor Oil |
| title_fullStr | Immobilization of Lipase on Agarose Beads for Enzymatic Hydrolsis/Transesterification of Castor Oil |
| title_full_unstemmed | Immobilization of Lipase on Agarose Beads for Enzymatic Hydrolsis/Transesterification of Castor Oil |
| title_short | Immobilization of Lipase on Agarose Beads for Enzymatic Hydrolsis/Transesterification of Castor Oil |
| title_sort | immobilization of lipase on agarose beads for enzymatic hydrolsis transesterification of castor oil |
| topic | agarose beads biocatalysis castor oil lipase ricinoleic acid |
| url | https://dergipark.org.tr/tr/download/article-file/3402331 |
| work_keys_str_mv | AT kubrakarakaya immobilizationoflipaseonagarosebeadsforenzymatichydrolsistransesterificationofcastoroil AT semrayılmazerkeskin immobilizationoflipaseonagarosebeadsforenzymatichydrolsistransesterificationofcastoroil |