Evaluation of Digital PCR as a Technique for Monitoring Acute Rejection in Kidney Transplantation
Early detection and proper management of kidney rejection are crucial for the long-term health of a transplant recipient. Recipients are normally monitored by serum creatinine measurement and sometimes with graft biopsies. Donor-derived cell-free deoxyribonucleic acid (cfDNA) in the recipient's...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BioMed Central
2017-03-01
|
| Series: | Genomics & Informatics |
| Subjects: | |
| Online Access: | http://genominfo.org/upload/pdf/gni-15-2.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832569563164704768 |
|---|---|
| author | Hyeseon Lee Young-Mi Park Yu-Mee We Duck Jong Han Jung-Woo Seo Haena Moon Yu-Ho Lee Yang-Gyun Kim Ju-Young Moon Sang-Ho Lee Jong-Keuk Lee |
| author_facet | Hyeseon Lee Young-Mi Park Yu-Mee We Duck Jong Han Jung-Woo Seo Haena Moon Yu-Ho Lee Yang-Gyun Kim Ju-Young Moon Sang-Ho Lee Jong-Keuk Lee |
| author_sort | Hyeseon Lee |
| collection | DOAJ |
| description | Early detection and proper management of kidney rejection are crucial for the long-term health of a transplant recipient. Recipients are normally monitored by serum creatinine measurement and sometimes with graft biopsies. Donor-derived cell-free deoxyribonucleic acid (cfDNA) in the recipient's plasma and/or urine may be a better indicator of acute rejection. We evaluated digital PCR (dPCR) as a system for monitoring graft status using single nucleotide polymorphism (SNP)-based detection of donor DNA in plasma or urine. We compared the detection abilities of the QX200, RainDrop, and QuantStudio 3D dPCR systems. The QX200 was the most accurate and sensitive. Plasma and/or urine samples were isolated from 34 kidney recipients at multiple time points after transplantation, and analyzed by dPCR using the QX200. We found that donor DNA was almost undetectable in plasma DNA samples, whereas a high percentage of donor DNA was measured in urine DNA samples, indicating that urine is a good source of cfDNA for patient monitoring. We found that at least 24% of the highly polymorphic SNPs used to identify individuals could also identify donor cfDNA in transplant patient samples. Our results further showed that autosomal, sex-specific, and mitochondrial SNPs were suitable markers for identifying donor cfDNA. Finally, we found that donor-derived cfDNA measurement by dPCR was not sufficient to predict a patient's clinical condition. Our results indicate that donor-derived cfDNA is not an accurate predictor of kidney status in kidney transplant patients. |
| format | Article |
| id | doaj-art-ef68acbf65874f7284e06b9640dabf00 |
| institution | Kabale University |
| issn | 1598-866X 2234-0742 |
| language | English |
| publishDate | 2017-03-01 |
| publisher | BioMed Central |
| record_format | Article |
| series | Genomics & Informatics |
| spelling | doaj-art-ef68acbf65874f7284e06b9640dabf002025-02-02T20:57:27ZengBioMed CentralGenomics & Informatics1598-866X2234-07422017-03-0115121010.5808/GI.2017.15.1.2203Evaluation of Digital PCR as a Technique for Monitoring Acute Rejection in Kidney TransplantationHyeseon Lee0Young-Mi Park1Yu-Mee We2Duck Jong Han3Jung-Woo Seo4Haena Moon5Yu-Ho Lee6Yang-Gyun Kim7Ju-Young Moon8Sang-Ho Lee9Jong-Keuk Lee10Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul 05505, Korea.Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul 05505, Korea.Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul 05505, Korea.Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Korea.Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Seoul 05278, Korea.Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Seoul 05278, Korea.Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Seoul 05278, Korea.Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Seoul 05278, Korea.Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Seoul 05278, Korea.Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Seoul 05278, Korea.Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul 05505, Korea.Early detection and proper management of kidney rejection are crucial for the long-term health of a transplant recipient. Recipients are normally monitored by serum creatinine measurement and sometimes with graft biopsies. Donor-derived cell-free deoxyribonucleic acid (cfDNA) in the recipient's plasma and/or urine may be a better indicator of acute rejection. We evaluated digital PCR (dPCR) as a system for monitoring graft status using single nucleotide polymorphism (SNP)-based detection of donor DNA in plasma or urine. We compared the detection abilities of the QX200, RainDrop, and QuantStudio 3D dPCR systems. The QX200 was the most accurate and sensitive. Plasma and/or urine samples were isolated from 34 kidney recipients at multiple time points after transplantation, and analyzed by dPCR using the QX200. We found that donor DNA was almost undetectable in plasma DNA samples, whereas a high percentage of donor DNA was measured in urine DNA samples, indicating that urine is a good source of cfDNA for patient monitoring. We found that at least 24% of the highly polymorphic SNPs used to identify individuals could also identify donor cfDNA in transplant patient samples. Our results further showed that autosomal, sex-specific, and mitochondrial SNPs were suitable markers for identifying donor cfDNA. Finally, we found that donor-derived cfDNA measurement by dPCR was not sufficient to predict a patient's clinical condition. Our results indicate that donor-derived cfDNA is not an accurate predictor of kidney status in kidney transplant patients.http://genominfo.org/upload/pdf/gni-15-2.pdfacute rejectioncell-free DNAdigital PCRkidney transplantation |
| spellingShingle | Hyeseon Lee Young-Mi Park Yu-Mee We Duck Jong Han Jung-Woo Seo Haena Moon Yu-Ho Lee Yang-Gyun Kim Ju-Young Moon Sang-Ho Lee Jong-Keuk Lee Evaluation of Digital PCR as a Technique for Monitoring Acute Rejection in Kidney Transplantation Genomics & Informatics acute rejection cell-free DNA digital PCR kidney transplantation |
| title | Evaluation of Digital PCR as a Technique for Monitoring Acute Rejection in Kidney Transplantation |
| title_full | Evaluation of Digital PCR as a Technique for Monitoring Acute Rejection in Kidney Transplantation |
| title_fullStr | Evaluation of Digital PCR as a Technique for Monitoring Acute Rejection in Kidney Transplantation |
| title_full_unstemmed | Evaluation of Digital PCR as a Technique for Monitoring Acute Rejection in Kidney Transplantation |
| title_short | Evaluation of Digital PCR as a Technique for Monitoring Acute Rejection in Kidney Transplantation |
| title_sort | evaluation of digital pcr as a technique for monitoring acute rejection in kidney transplantation |
| topic | acute rejection cell-free DNA digital PCR kidney transplantation |
| url | http://genominfo.org/upload/pdf/gni-15-2.pdf |
| work_keys_str_mv | AT hyeseonlee evaluationofdigitalpcrasatechniqueformonitoringacuterejectioninkidneytransplantation AT youngmipark evaluationofdigitalpcrasatechniqueformonitoringacuterejectioninkidneytransplantation AT yumeewe evaluationofdigitalpcrasatechniqueformonitoringacuterejectioninkidneytransplantation AT duckjonghan evaluationofdigitalpcrasatechniqueformonitoringacuterejectioninkidneytransplantation AT jungwooseo evaluationofdigitalpcrasatechniqueformonitoringacuterejectioninkidneytransplantation AT haenamoon evaluationofdigitalpcrasatechniqueformonitoringacuterejectioninkidneytransplantation AT yuholee evaluationofdigitalpcrasatechniqueformonitoringacuterejectioninkidneytransplantation AT yanggyunkim evaluationofdigitalpcrasatechniqueformonitoringacuterejectioninkidneytransplantation AT juyoungmoon evaluationofdigitalpcrasatechniqueformonitoringacuterejectioninkidneytransplantation AT sangholee evaluationofdigitalpcrasatechniqueformonitoringacuterejectioninkidneytransplantation AT jongkeuklee evaluationofdigitalpcrasatechniqueformonitoringacuterejectioninkidneytransplantation |