Antibacterial activity of ciprofloxacin-impregnated 3D-printed polylactic acid discs: an in vitro study
Introduction: Three-dimensional (3D) printing technology allows incorporation of various substances including antibiotics into different structures. This study aimed to evaluate the antibacterial activity of ciprofloxacin-impregnated 3D discs against Escherichia coli. Methodology: Polylactic aci...
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The Journal of Infection in Developing Countries
2022-03-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/15267 |
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| author | Emrah Ruh Emil Mammadov |
| author_facet | Emrah Ruh Emil Mammadov |
| author_sort | Emrah Ruh |
| collection | DOAJ |
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Introduction: Three-dimensional (3D) printing technology allows incorporation of various substances including antibiotics into different structures. This study aimed to evaluate the antibacterial activity of ciprofloxacin-impregnated 3D discs against Escherichia coli.
Methodology: Polylactic acid pellets were coated with ciprofloxacin at 1% and 2% concentrations, then filaments were produced from these pellets, and antibiotic-containing discs were obtained using fused deposition modeling 3D printers. The working temperatures during filament extrusion and 3D printing processes were 200 °C and 215 °C, respectively. Therefore, in order to test the thermal stability of ciprofloxacin during these processes, the antibiotic was exposed to 200 °C and 215 °C in an oven, and then tested against E. coli. Following this, efficiencies of antibiotic-coated pellets, filaments and discs against E. coli were determined by diffusion tests.
Results: Ciprofloxacin heated at 200 °C and 215 °C was stable and retained its antibacterial activity. Pellets, filaments and discs coated with 1% or 2% concentration of ciprofloxacin produced inhibition zones in the culture plates. Increasing ciprofloxacin concentration did not significantly affect the diameter of inhibition zones (p > 0.05). Ciprofloxacin-containing polylactic acid pellets produced significantly larger inhibition zones than those of filaments and discs (p < 0.0001). The difference in zone diameters around ciprofloxacin-containing filaments and discs was not statistically significant (p > 0.05).
Conclusions: Ciprofloxacin-coated polylactic acid-based 3D discs displayed antibacterial activity against E. coli. This suggests that, various polylactic acid-based ciprofloxacin-containing 3D products can be obtained and evaluated for antibacterial activity in future studies.
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| format | Article |
| id | doaj-art-ef64638315094deaa2d67f2ae86c31f9 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-ef64638315094deaa2d67f2ae86c31f92025-08-20T02:27:22ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802022-03-01160310.3855/jidc.15267Antibacterial activity of ciprofloxacin-impregnated 3D-printed polylactic acid discs: an in vitro studyEmrah Ruh0Emil Mammadov1Department of Medical Microbiology and Clinical Microbiology, Faculty of Medicine, Near East University, Nicosia, Northern CyprusDepartment of Pediatric Surgery, Faculty of Medicine, Near East University, Nicosia, Northern Cyprus Introduction: Three-dimensional (3D) printing technology allows incorporation of various substances including antibiotics into different structures. This study aimed to evaluate the antibacterial activity of ciprofloxacin-impregnated 3D discs against Escherichia coli. Methodology: Polylactic acid pellets were coated with ciprofloxacin at 1% and 2% concentrations, then filaments were produced from these pellets, and antibiotic-containing discs were obtained using fused deposition modeling 3D printers. The working temperatures during filament extrusion and 3D printing processes were 200 °C and 215 °C, respectively. Therefore, in order to test the thermal stability of ciprofloxacin during these processes, the antibiotic was exposed to 200 °C and 215 °C in an oven, and then tested against E. coli. Following this, efficiencies of antibiotic-coated pellets, filaments and discs against E. coli were determined by diffusion tests. Results: Ciprofloxacin heated at 200 °C and 215 °C was stable and retained its antibacterial activity. Pellets, filaments and discs coated with 1% or 2% concentration of ciprofloxacin produced inhibition zones in the culture plates. Increasing ciprofloxacin concentration did not significantly affect the diameter of inhibition zones (p > 0.05). Ciprofloxacin-containing polylactic acid pellets produced significantly larger inhibition zones than those of filaments and discs (p < 0.0001). The difference in zone diameters around ciprofloxacin-containing filaments and discs was not statistically significant (p > 0.05). Conclusions: Ciprofloxacin-coated polylactic acid-based 3D discs displayed antibacterial activity against E. coli. This suggests that, various polylactic acid-based ciprofloxacin-containing 3D products can be obtained and evaluated for antibacterial activity in future studies. https://jidc.org/index.php/journal/article/view/15267CiprofloxacinEscherichia coliprintingdisc |
| spellingShingle | Emrah Ruh Emil Mammadov Antibacterial activity of ciprofloxacin-impregnated 3D-printed polylactic acid discs: an in vitro study Journal of Infection in Developing Countries Ciprofloxacin Escherichia coli printing disc |
| title | Antibacterial activity of ciprofloxacin-impregnated 3D-printed polylactic acid discs: an in vitro study |
| title_full | Antibacterial activity of ciprofloxacin-impregnated 3D-printed polylactic acid discs: an in vitro study |
| title_fullStr | Antibacterial activity of ciprofloxacin-impregnated 3D-printed polylactic acid discs: an in vitro study |
| title_full_unstemmed | Antibacterial activity of ciprofloxacin-impregnated 3D-printed polylactic acid discs: an in vitro study |
| title_short | Antibacterial activity of ciprofloxacin-impregnated 3D-printed polylactic acid discs: an in vitro study |
| title_sort | antibacterial activity of ciprofloxacin impregnated 3d printed polylactic acid discs an in vitro study |
| topic | Ciprofloxacin Escherichia coli printing disc |
| url | https://jidc.org/index.php/journal/article/view/15267 |
| work_keys_str_mv | AT emrahruh antibacterialactivityofciprofloxacinimpregnated3dprintedpolylacticaciddiscsaninvitrostudy AT emilmammadov antibacterialactivityofciprofloxacinimpregnated3dprintedpolylacticaciddiscsaninvitrostudy |