Single-cell transcriptomics unveils multifaceted immune heterogeneity in early-onset versus late-onset cervical cancer
Abstract Early-onset (EOCC) and late-onset cervical cancers (LOCC) represent two clinically distinct subtypes, each defined by unique clinical manifestations and therapeutic responses. However, their immunological profiles remain poorly explored. Herein, we analyzed single-cell transcriptomic data f...
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| Language: | English |
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BMC
2025-01-01
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| Series: | World Journal of Surgical Oncology |
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| Online Access: | https://doi.org/10.1186/s12957-025-03654-z |
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| author | Qian Chen Dongfeng Deng Hong Zhu Shan Li |
| author_facet | Qian Chen Dongfeng Deng Hong Zhu Shan Li |
| author_sort | Qian Chen |
| collection | DOAJ |
| description | Abstract Early-onset (EOCC) and late-onset cervical cancers (LOCC) represent two clinically distinct subtypes, each defined by unique clinical manifestations and therapeutic responses. However, their immunological profiles remain poorly explored. Herein, we analyzed single-cell transcriptomic data from 4 EOCC and 4 LOCC samples to compare their immune architectures. Epithelial cells in EOCC exhibited a notable dual immunological phenotype, characterized by immune-suppressive properties driven by elevated CXCL production, alongside immune-stimulatory features linked to heightened HLA molecule expression. CD4 + and CD8 + T cells in LOCC demonstrated a heightened activation state, while NK cells exhibited diminished cytotoxicity. Macrophages in LOCC displayed enhanced polarization towards both M1 and M2 phenotypes, along with dendritic cells showing augmented antigen-presenting capacity. Regarding cancer-associated fibroblasts (CAFs), EOCC was enriched with inflammatory CAFs, whereas LOCC harbored a higher proportion of antigen-presenting CAFs. These findings reveal the multifaceted immune heterogeneity between EOCC and LOCC, underscoring the imperative for age-tailored immunotherapeutic strategies. |
| format | Article |
| id | doaj-art-ef62ee734fb641f0a3b4888624c85f7b |
| institution | Kabale University |
| issn | 1477-7819 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj-art-ef62ee734fb641f0a3b4888624c85f7b2025-01-19T12:25:50ZengBMCWorld Journal of Surgical Oncology1477-78192025-01-0123111710.1186/s12957-025-03654-zSingle-cell transcriptomics unveils multifaceted immune heterogeneity in early-onset versus late-onset cervical cancerQian Chen0Dongfeng Deng1Hong Zhu2Shan Li3Department of Oncology, Xiangya Hospital, Central South UniversityDepartment of Oncology, Hunan University of Medicine General HospitalDepartment of Oncology, Xiangya Hospital, Central South UniversityDepartment of Oncology, Xiangya Hospital, Central South UniversityAbstract Early-onset (EOCC) and late-onset cervical cancers (LOCC) represent two clinically distinct subtypes, each defined by unique clinical manifestations and therapeutic responses. However, their immunological profiles remain poorly explored. Herein, we analyzed single-cell transcriptomic data from 4 EOCC and 4 LOCC samples to compare their immune architectures. Epithelial cells in EOCC exhibited a notable dual immunological phenotype, characterized by immune-suppressive properties driven by elevated CXCL production, alongside immune-stimulatory features linked to heightened HLA molecule expression. CD4 + and CD8 + T cells in LOCC demonstrated a heightened activation state, while NK cells exhibited diminished cytotoxicity. Macrophages in LOCC displayed enhanced polarization towards both M1 and M2 phenotypes, along with dendritic cells showing augmented antigen-presenting capacity. Regarding cancer-associated fibroblasts (CAFs), EOCC was enriched with inflammatory CAFs, whereas LOCC harbored a higher proportion of antigen-presenting CAFs. These findings reveal the multifaceted immune heterogeneity between EOCC and LOCC, underscoring the imperative for age-tailored immunotherapeutic strategies.https://doi.org/10.1186/s12957-025-03654-zCervical cancerAgeSingle-cell RNA sequencingImmune heterogeneity |
| spellingShingle | Qian Chen Dongfeng Deng Hong Zhu Shan Li Single-cell transcriptomics unveils multifaceted immune heterogeneity in early-onset versus late-onset cervical cancer World Journal of Surgical Oncology Cervical cancer Age Single-cell RNA sequencing Immune heterogeneity |
| title | Single-cell transcriptomics unveils multifaceted immune heterogeneity in early-onset versus late-onset cervical cancer |
| title_full | Single-cell transcriptomics unveils multifaceted immune heterogeneity in early-onset versus late-onset cervical cancer |
| title_fullStr | Single-cell transcriptomics unveils multifaceted immune heterogeneity in early-onset versus late-onset cervical cancer |
| title_full_unstemmed | Single-cell transcriptomics unveils multifaceted immune heterogeneity in early-onset versus late-onset cervical cancer |
| title_short | Single-cell transcriptomics unveils multifaceted immune heterogeneity in early-onset versus late-onset cervical cancer |
| title_sort | single cell transcriptomics unveils multifaceted immune heterogeneity in early onset versus late onset cervical cancer |
| topic | Cervical cancer Age Single-cell RNA sequencing Immune heterogeneity |
| url | https://doi.org/10.1186/s12957-025-03654-z |
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