Developmental Validation of DNA Quantitation System, Extended STR Typing Multiplex, and Database Solutions for <i>Panthera leo</i> Genotyping
This study describes the development of a species determination/DNA quantification system called Pleo Qplex and an individual identification STR multiplex called Pleo STRplex using <i>Panthera leo</i> samples. Pleo Qplex enables us to measure the quantity of extracted nuclear and mitocho...
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MDPI AG
2025-04-01
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| author | Lenka Vankova Johana Alaverdyan Daniel Vanek |
| author_facet | Lenka Vankova Johana Alaverdyan Daniel Vanek |
| author_sort | Lenka Vankova |
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| description | This study describes the development of a species determination/DNA quantification system called Pleo Qplex and an individual identification STR multiplex called Pleo STRplex using <i>Panthera leo</i> samples. Pleo Qplex enables us to measure the quantity of extracted nuclear and mitochondrial DNA and detect the presence of co-purified inhibitors. Pleo STRplex, consisting of seven loci, enables the determination of the DNA profile from a sample of <i>Panthera leo</i> based on the analysis of short tandem repeats (STRs). The Pleo STRplex provides additional loci on top of previously published STR loci in Ptig STRplex and contains a specific STR marker that confirms <i>Panthera leo</i>. An allelic ladder of all STR markers was prepared to enable reliable allele calling. The STR loci can also be used to type the DNA of other members of the genus Panthera. The work on the resulting STR profiles is performed using GenoProof Suite, which offers databasing, matching, and relationship analysis. |
| format | Article |
| id | doaj-art-ef6073e0048a4b52b7fd516e1a162c8d |
| institution | OA Journals |
| issn | 2075-1729 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj-art-ef6073e0048a4b52b7fd516e1a162c8d2025-08-20T02:28:15ZengMDPI AGLife2075-17292025-04-0115466410.3390/life15040664Developmental Validation of DNA Quantitation System, Extended STR Typing Multiplex, and Database Solutions for <i>Panthera leo</i> GenotypingLenka Vankova0Johana Alaverdyan1Daniel Vanek2Institute for Environmental Sciences, Charles University Prague, Benatska 2, 12800 Prague, Czech RepublicInstitute for Environmental Sciences, Charles University Prague, Benatska 2, 12800 Prague, Czech RepublicInstitute for Environmental Sciences, Charles University Prague, Benatska 2, 12800 Prague, Czech RepublicThis study describes the development of a species determination/DNA quantification system called Pleo Qplex and an individual identification STR multiplex called Pleo STRplex using <i>Panthera leo</i> samples. Pleo Qplex enables us to measure the quantity of extracted nuclear and mitochondrial DNA and detect the presence of co-purified inhibitors. Pleo STRplex, consisting of seven loci, enables the determination of the DNA profile from a sample of <i>Panthera leo</i> based on the analysis of short tandem repeats (STRs). The Pleo STRplex provides additional loci on top of previously published STR loci in Ptig STRplex and contains a specific STR marker that confirms <i>Panthera leo</i>. An allelic ladder of all STR markers was prepared to enable reliable allele calling. The STR loci can also be used to type the DNA of other members of the genus Panthera. The work on the resulting STR profiles is performed using GenoProof Suite, which offers databasing, matching, and relationship analysis.https://www.mdpi.com/2075-1729/15/4/664DNA quantitationspecies determinationSTR multiplexCITES organismforensic geneticsdatabasing |
| spellingShingle | Lenka Vankova Johana Alaverdyan Daniel Vanek Developmental Validation of DNA Quantitation System, Extended STR Typing Multiplex, and Database Solutions for <i>Panthera leo</i> Genotyping Life DNA quantitation species determination STR multiplex CITES organism forensic genetics databasing |
| title | Developmental Validation of DNA Quantitation System, Extended STR Typing Multiplex, and Database Solutions for <i>Panthera leo</i> Genotyping |
| title_full | Developmental Validation of DNA Quantitation System, Extended STR Typing Multiplex, and Database Solutions for <i>Panthera leo</i> Genotyping |
| title_fullStr | Developmental Validation of DNA Quantitation System, Extended STR Typing Multiplex, and Database Solutions for <i>Panthera leo</i> Genotyping |
| title_full_unstemmed | Developmental Validation of DNA Quantitation System, Extended STR Typing Multiplex, and Database Solutions for <i>Panthera leo</i> Genotyping |
| title_short | Developmental Validation of DNA Quantitation System, Extended STR Typing Multiplex, and Database Solutions for <i>Panthera leo</i> Genotyping |
| title_sort | developmental validation of dna quantitation system extended str typing multiplex and database solutions for i panthera leo i genotyping |
| topic | DNA quantitation species determination STR multiplex CITES organism forensic genetics databasing |
| url | https://www.mdpi.com/2075-1729/15/4/664 |
| work_keys_str_mv | AT lenkavankova developmentalvalidationofdnaquantitationsystemextendedstrtypingmultiplexanddatabasesolutionsforipantheraleoigenotyping AT johanaalaverdyan developmentalvalidationofdnaquantitationsystemextendedstrtypingmultiplexanddatabasesolutionsforipantheraleoigenotyping AT danielvanek developmentalvalidationofdnaquantitationsystemextendedstrtypingmultiplexanddatabasesolutionsforipantheraleoigenotyping |