Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular Hypertension
Glaucoma is a neurodegenerative disease characterized by the loss of retinal ganglion cells (RGCs), with intraocular pressure (IOP) being its primary risk factor. Despite controlling IOP, the neurodegenerative process often continues. Therefore, substances with neuroprotective, antioxidant, and anti...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/14/1/4 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589367763271680 |
|---|---|
| author | José A. Matamoros Sara Rubio-Casado José A. Fernández-Albarral Miguel A. Martínez-López Elena Salobrar-García Eva M. Marco Victor Paleo-García Rosa de Hoz Inés López-Cuenca Lorena Elvira-Hurtado Lidia Sánchez-Puebla José M. Ramírez Juan J. Salazar Meritxell López-Gallardo Ana I. Ramírez |
| author_facet | José A. Matamoros Sara Rubio-Casado José A. Fernández-Albarral Miguel A. Martínez-López Elena Salobrar-García Eva M. Marco Victor Paleo-García Rosa de Hoz Inés López-Cuenca Lorena Elvira-Hurtado Lidia Sánchez-Puebla José M. Ramírez Juan J. Salazar Meritxell López-Gallardo Ana I. Ramírez |
| author_sort | José A. Matamoros |
| collection | DOAJ |
| description | Glaucoma is a neurodegenerative disease characterized by the loss of retinal ganglion cells (RGCs), with intraocular pressure (IOP) being its primary risk factor. Despite controlling IOP, the neurodegenerative process often continues. Therefore, substances with neuroprotective, antioxidant, and anti-inflammatory properties could protect against RGC death. This study investigated the neuroprotective effects on RGCs and visual pathway neurons of a compound consisting of citicoline and coenzyme Q10 (CoQ10) in a mouse model of unilateral, laser-induced ocular hypertension (OHT). Four groups of mice were used: vehicle group (<i>n</i> = 6), citicoline + CoQ10 group (<i>n</i> = 6), laser–vehicle group (<i>n</i> = 6), and laser–citicoline + CoQ10 group (<i>n</i> = 6). The citicoline + CoQ10 was administered orally once a day starting 15 days before laser treatment, continuing until sacrifice (7 days post-laser). Retinas, the dorsolateral geniculate nucleus (dLGN), the superior colliculus (SC), and the visual cortex (V1) were analyzed. The citicoline + CoQ10 compound used in the laser–citicoline + CoQ10 group demonstrated (1) an ocular hypotensive effect only at 24 h post-laser; (2) prevention of Brn3a+ RGC death in OHT eyes; and (3) no changes in NeuN+ neurons in the dLGN. This study demonstrates that the oral administration of the citicoline + CoQ10 combination may exert a neuroprotective effect against RGC death in an established rodent model of OHT. |
| format | Article |
| id | doaj-art-ef4384647b6c4f888f1c26d19942d5e2 |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-ef4384647b6c4f888f1c26d19942d5e22025-01-24T13:19:04ZengMDPI AGAntioxidants2076-39212024-12-01141410.3390/antiox14010004Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular HypertensionJosé A. Matamoros0Sara Rubio-Casado1José A. Fernández-Albarral2Miguel A. Martínez-López3Elena Salobrar-García4Eva M. Marco5Victor Paleo-García6Rosa de Hoz7Inés López-Cuenca8Lorena Elvira-Hurtado9Lidia Sánchez-Puebla10José M. Ramírez11Juan J. Salazar12Meritxell López-Gallardo13Ana I. Ramírez14Ramon Castroviejo Institute for Ophthalmic Research, Complutense University of Madrid, 28040 Madrid, SpainRamon Castroviejo Institute for Ophthalmic Research, Complutense University of Madrid, 28040 Madrid, SpainRamon Castroviejo Institute for Ophthalmic Research, Complutense University of Madrid, 28040 Madrid, SpainRamon Castroviejo Institute for Ophthalmic Research, Complutense University of Madrid, 28040 Madrid, SpainRamon Castroviejo Institute for Ophthalmic Research, Complutense University of Madrid, 28040 Madrid, SpainRamon Castroviejo Institute for Ophthalmic Research, Complutense University of Madrid, 28040 Madrid, SpainDepartment of Physiology, Faculty of Medicine, Complutense University of Madrid, 28040 Madrid, SpainRamon Castroviejo Institute for Ophthalmic Research, Complutense University of Madrid, 28040 Madrid, SpainRamon Castroviejo Institute for Ophthalmic Research, Complutense University of Madrid, 28040 Madrid, SpainRamon Castroviejo Institute for Ophthalmic Research, Complutense University of Madrid, 28040 Madrid, SpainRamon Castroviejo Institute for Ophthalmic Research, Complutense University of Madrid, 28040 Madrid, SpainRamon Castroviejo Institute for Ophthalmic Research, Complutense University of Madrid, 28040 Madrid, SpainRamon Castroviejo Institute for Ophthalmic Research, Complutense University of Madrid, 28040 Madrid, SpainRamon Castroviejo Institute for Ophthalmic Research, Complutense University of Madrid, 28040 Madrid, SpainRamon Castroviejo Institute for Ophthalmic Research, Complutense University of Madrid, 28040 Madrid, SpainGlaucoma is a neurodegenerative disease characterized by the loss of retinal ganglion cells (RGCs), with intraocular pressure (IOP) being its primary risk factor. Despite controlling IOP, the neurodegenerative process often continues. Therefore, substances with neuroprotective, antioxidant, and anti-inflammatory properties could protect against RGC death. This study investigated the neuroprotective effects on RGCs and visual pathway neurons of a compound consisting of citicoline and coenzyme Q10 (CoQ10) in a mouse model of unilateral, laser-induced ocular hypertension (OHT). Four groups of mice were used: vehicle group (<i>n</i> = 6), citicoline + CoQ10 group (<i>n</i> = 6), laser–vehicle group (<i>n</i> = 6), and laser–citicoline + CoQ10 group (<i>n</i> = 6). The citicoline + CoQ10 was administered orally once a day starting 15 days before laser treatment, continuing until sacrifice (7 days post-laser). Retinas, the dorsolateral geniculate nucleus (dLGN), the superior colliculus (SC), and the visual cortex (V1) were analyzed. The citicoline + CoQ10 compound used in the laser–citicoline + CoQ10 group demonstrated (1) an ocular hypotensive effect only at 24 h post-laser; (2) prevention of Brn3a+ RGC death in OHT eyes; and (3) no changes in NeuN+ neurons in the dLGN. This study demonstrates that the oral administration of the citicoline + CoQ10 combination may exert a neuroprotective effect against RGC death in an established rodent model of OHT.https://www.mdpi.com/2076-3921/14/1/4ganglion cellsretinavisual pathwayexperimental glaucomaciticolinecoenzyme Q10 |
| spellingShingle | José A. Matamoros Sara Rubio-Casado José A. Fernández-Albarral Miguel A. Martínez-López Elena Salobrar-García Eva M. Marco Victor Paleo-García Rosa de Hoz Inés López-Cuenca Lorena Elvira-Hurtado Lidia Sánchez-Puebla José M. Ramírez Juan J. Salazar Meritxell López-Gallardo Ana I. Ramírez Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular Hypertension Antioxidants ganglion cells retina visual pathway experimental glaucoma citicoline coenzyme Q10 |
| title | Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular Hypertension |
| title_full | Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular Hypertension |
| title_fullStr | Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular Hypertension |
| title_full_unstemmed | Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular Hypertension |
| title_short | Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular Hypertension |
| title_sort | neuroprotective effect of the combination of citicoline and coq10 in a mouse model of ocular hypertension |
| topic | ganglion cells retina visual pathway experimental glaucoma citicoline coenzyme Q10 |
| url | https://www.mdpi.com/2076-3921/14/1/4 |
| work_keys_str_mv | AT joseamatamoros neuroprotectiveeffectofthecombinationofciticolineandcoq10inamousemodelofocularhypertension AT sararubiocasado neuroprotectiveeffectofthecombinationofciticolineandcoq10inamousemodelofocularhypertension AT joseafernandezalbarral neuroprotectiveeffectofthecombinationofciticolineandcoq10inamousemodelofocularhypertension AT miguelamartinezlopez neuroprotectiveeffectofthecombinationofciticolineandcoq10inamousemodelofocularhypertension AT elenasalobrargarcia neuroprotectiveeffectofthecombinationofciticolineandcoq10inamousemodelofocularhypertension AT evammarco neuroprotectiveeffectofthecombinationofciticolineandcoq10inamousemodelofocularhypertension AT victorpaleogarcia neuroprotectiveeffectofthecombinationofciticolineandcoq10inamousemodelofocularhypertension AT rosadehoz neuroprotectiveeffectofthecombinationofciticolineandcoq10inamousemodelofocularhypertension AT ineslopezcuenca neuroprotectiveeffectofthecombinationofciticolineandcoq10inamousemodelofocularhypertension AT lorenaelvirahurtado neuroprotectiveeffectofthecombinationofciticolineandcoq10inamousemodelofocularhypertension AT lidiasanchezpuebla neuroprotectiveeffectofthecombinationofciticolineandcoq10inamousemodelofocularhypertension AT josemramirez neuroprotectiveeffectofthecombinationofciticolineandcoq10inamousemodelofocularhypertension AT juanjsalazar neuroprotectiveeffectofthecombinationofciticolineandcoq10inamousemodelofocularhypertension AT meritxelllopezgallardo neuroprotectiveeffectofthecombinationofciticolineandcoq10inamousemodelofocularhypertension AT anairamirez neuroprotectiveeffectofthecombinationofciticolineandcoq10inamousemodelofocularhypertension |