Artificial Induction of Meiotic Gynogenesis in <i>Koi</i> Carp Using Blunt Snout Bream Sperm and Identification of Gynogenetic Offspring
Artificial gynogenesis is an effective means of producing pure lines and is widely used for genetic analysis of fish and for sex control. In this study, inactivated sperm from heterogenous blunt snout bream (<i>Megalobrama amblycephala</i>, MA) were used to activate <i>Kohaku koi&l...
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2025-05-01
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| author | Xiaoyu Chen Xiulan Shi Jun Guo Kai Lin Mingkun Luo Zaijie Dong |
| author_facet | Xiaoyu Chen Xiulan Shi Jun Guo Kai Lin Mingkun Luo Zaijie Dong |
| author_sort | Xiaoyu Chen |
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| description | Artificial gynogenesis is an effective means of producing pure lines and is widely used for genetic analysis of fish and for sex control. In this study, inactivated sperm from heterogenous blunt snout bream (<i>Megalobrama amblycephala</i>, MA) were used to activate <i>Kohaku koi</i> (<i>Cyprinus carpio</i> var. <i>koi</i>, CK) and produce high-quality female offspring. To determine whether the offspring were gynogenetic fish, the karyotype and DNA content of the CK, MA and the induced offspring (IO) were first compared and it was found that the IO were diploid with 100 chromosomes and their karyotype was 22m + 34sm + 22st + 22t. The DNA content of the IO was not significantly different from that of the CK. Subsequently, the amplified band profiles of CK, MA and IO were analyzed with species-specific microsatellite markers. The results showed that there were no amplified MA microsatellite bands in IO. The size of the amplified bands and the sequence of the 5S rDNA in CK, MA and IO were also analyzed. It was found that the amplified 5S rDNA gene fragments in IO contained two fragments that were both the same size as those of CK and matched more than 90% with those of CK. Finally, the sex of IO was verified using gonadal tissue sections. The result showed that IO was not an all-female population; males were also present (36.7%). In summary, a series of validation methods confirmed that the induced offspring were gynogenetic fish, which is the basis for the subsequent genetic improvement of pure lines of high-quality <i>koi</i>. |
| format | Article |
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| institution | Kabale University |
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| spelling | doaj-art-ef3c5496925f416aa9ce76db30a93d182025-08-20T03:47:48ZengMDPI AGAnimals2076-26152025-05-011510141110.3390/ani15101411Artificial Induction of Meiotic Gynogenesis in <i>Koi</i> Carp Using Blunt Snout Bream Sperm and Identification of Gynogenetic OffspringXiaoyu Chen0Xiulan Shi1Jun Guo2Kai Lin3Mingkun Luo4Zaijie Dong5Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaJiangsu Qihong Ecological Agriculture Development Co., Ltd., Suzhou 215416, ChinaJiangsu Qihong Ecological Agriculture Development Co., Ltd., Suzhou 215416, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaArtificial gynogenesis is an effective means of producing pure lines and is widely used for genetic analysis of fish and for sex control. In this study, inactivated sperm from heterogenous blunt snout bream (<i>Megalobrama amblycephala</i>, MA) were used to activate <i>Kohaku koi</i> (<i>Cyprinus carpio</i> var. <i>koi</i>, CK) and produce high-quality female offspring. To determine whether the offspring were gynogenetic fish, the karyotype and DNA content of the CK, MA and the induced offspring (IO) were first compared and it was found that the IO were diploid with 100 chromosomes and their karyotype was 22m + 34sm + 22st + 22t. The DNA content of the IO was not significantly different from that of the CK. Subsequently, the amplified band profiles of CK, MA and IO were analyzed with species-specific microsatellite markers. The results showed that there were no amplified MA microsatellite bands in IO. The size of the amplified bands and the sequence of the 5S rDNA in CK, MA and IO were also analyzed. It was found that the amplified 5S rDNA gene fragments in IO contained two fragments that were both the same size as those of CK and matched more than 90% with those of CK. Finally, the sex of IO was verified using gonadal tissue sections. The result showed that IO was not an all-female population; males were also present (36.7%). In summary, a series of validation methods confirmed that the induced offspring were gynogenetic fish, which is the basis for the subsequent genetic improvement of pure lines of high-quality <i>koi</i>.https://www.mdpi.com/2076-2615/15/10/1411gynogenesis<i>koi</i> carpmicrosatellitechromosome5S rDNA |
| spellingShingle | Xiaoyu Chen Xiulan Shi Jun Guo Kai Lin Mingkun Luo Zaijie Dong Artificial Induction of Meiotic Gynogenesis in <i>Koi</i> Carp Using Blunt Snout Bream Sperm and Identification of Gynogenetic Offspring Animals gynogenesis <i>koi</i> carp microsatellite chromosome 5S rDNA |
| title | Artificial Induction of Meiotic Gynogenesis in <i>Koi</i> Carp Using Blunt Snout Bream Sperm and Identification of Gynogenetic Offspring |
| title_full | Artificial Induction of Meiotic Gynogenesis in <i>Koi</i> Carp Using Blunt Snout Bream Sperm and Identification of Gynogenetic Offspring |
| title_fullStr | Artificial Induction of Meiotic Gynogenesis in <i>Koi</i> Carp Using Blunt Snout Bream Sperm and Identification of Gynogenetic Offspring |
| title_full_unstemmed | Artificial Induction of Meiotic Gynogenesis in <i>Koi</i> Carp Using Blunt Snout Bream Sperm and Identification of Gynogenetic Offspring |
| title_short | Artificial Induction of Meiotic Gynogenesis in <i>Koi</i> Carp Using Blunt Snout Bream Sperm and Identification of Gynogenetic Offspring |
| title_sort | artificial induction of meiotic gynogenesis in i koi i carp using blunt snout bream sperm and identification of gynogenetic offspring |
| topic | gynogenesis <i>koi</i> carp microsatellite chromosome 5S rDNA |
| url | https://www.mdpi.com/2076-2615/15/10/1411 |
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