Genome-wide identification of the nuclear redox protein gene family revealed its potential role in drought stress tolerance in rice
IntroductionThioredoxins (TRX) are redox-active proteins critical for plant stress adaptation. As a TRX family member, nucleoredoxin (NRX) maintains drought-induced redox homeostasis, yet its genome-wide characterization in rice remains uninvestigated.MethodsUsing HMMER3.0 (E-value <1e-5) and...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-04-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1562718/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850178707915800576 |
|---|---|
| author | Zijie Liu Xingfei Zheng Dabing Yang Lanzhi Li Hexing Yin |
| author_facet | Zijie Liu Xingfei Zheng Dabing Yang Lanzhi Li Hexing Yin |
| author_sort | Zijie Liu |
| collection | DOAJ |
| description | IntroductionThioredoxins (TRX) are redox-active proteins critical for plant stress adaptation. As a TRX family member, nucleoredoxin (NRX) maintains drought-induced redox homeostasis, yet its genome-wide characterization in rice remains uninvestigated.MethodsUsing HMMER3.0 (E-value <1e-5) and TBtools, we identified OsNRX genes across three rice varieties (Minghui63, Nipponbare, 9311). Conserved domains were verified by SMART/CDD, while promoter cis-elements were systematically predicted with PlantCARE. Tissue-specific expression patterns were analyzed using RiceXPro data, and drought responses were quantified via qRT-PCR in drought-tolerant (Jiangnong Zao 1B) versus sensitive (TAISEN GLUTINOUS YU 1157) varieties under PEG6000 stress.ResultsTen OsNRX genes were classified into three subfamilies (NRX1/NRX2/NRX3) exhibiting conserved domain architectures. Promoter analysis identified abundant stress-responsive elements (ABRE, MBS) and phytohormone signals (ABA/JA/SA). Tissue-specific expression profiles revealed NRX1a dominance in roots/hulls, versus NRX1b/NRX2 enrichment in endosperm. Drought stress triggered rapid OsNRX upregulation (20-53-fold within 3-6h), with tolerant varieties showing earlier NRX2 activation than sensitive counterparts.DiscussionOsNRX genes exhibit dynamic drought-responsive regulation, while their spatiotemporal expression in glumes, embryos, and endosperm suggests potential dual roles in stress adaptation and grain development. These results provide molecular targets for improving drought resilience in rice breeding. |
| format | Article |
| id | doaj-art-ef2ffb637dd44debaf7b03d69592f6cd |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-ef2ffb637dd44debaf7b03d69592f6cd2025-08-20T02:18:39ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-04-011610.3389/fpls.2025.15627181562718Genome-wide identification of the nuclear redox protein gene family revealed its potential role in drought stress tolerance in riceZijie Liu0Xingfei Zheng1Dabing Yang2Lanzhi Li3Hexing Yin4Hunan Engineering & Technology Research Center for Agricultural Big Data Analysis & Decision-Making, College of Plant Protection, Hunan Agricultural University, Changsha, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crop Institute, Hubei Academy of Agricultural Sciences, Wuhan, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crop Institute, Hubei Academy of Agricultural Sciences, Wuhan, ChinaHunan Engineering & Technology Research Center for Agricultural Big Data Analysis & Decision-Making, College of Plant Protection, Hunan Agricultural University, Changsha, ChinaCrop Phenomics Research Center, Huazhi Biotechnology Co., Ltd, Changsha, ChinaIntroductionThioredoxins (TRX) are redox-active proteins critical for plant stress adaptation. As a TRX family member, nucleoredoxin (NRX) maintains drought-induced redox homeostasis, yet its genome-wide characterization in rice remains uninvestigated.MethodsUsing HMMER3.0 (E-value <1e-5) and TBtools, we identified OsNRX genes across three rice varieties (Minghui63, Nipponbare, 9311). Conserved domains were verified by SMART/CDD, while promoter cis-elements were systematically predicted with PlantCARE. Tissue-specific expression patterns were analyzed using RiceXPro data, and drought responses were quantified via qRT-PCR in drought-tolerant (Jiangnong Zao 1B) versus sensitive (TAISEN GLUTINOUS YU 1157) varieties under PEG6000 stress.ResultsTen OsNRX genes were classified into three subfamilies (NRX1/NRX2/NRX3) exhibiting conserved domain architectures. Promoter analysis identified abundant stress-responsive elements (ABRE, MBS) and phytohormone signals (ABA/JA/SA). Tissue-specific expression profiles revealed NRX1a dominance in roots/hulls, versus NRX1b/NRX2 enrichment in endosperm. Drought stress triggered rapid OsNRX upregulation (20-53-fold within 3-6h), with tolerant varieties showing earlier NRX2 activation than sensitive counterparts.DiscussionOsNRX genes exhibit dynamic drought-responsive regulation, while their spatiotemporal expression in glumes, embryos, and endosperm suggests potential dual roles in stress adaptation and grain development. These results provide molecular targets for improving drought resilience in rice breeding.https://www.frontiersin.org/articles/10.3389/fpls.2025.1562718/fullricenucleoredoxin gene familydrought stressgenome-wide identificationexpression pattern |
| spellingShingle | Zijie Liu Xingfei Zheng Dabing Yang Lanzhi Li Hexing Yin Genome-wide identification of the nuclear redox protein gene family revealed its potential role in drought stress tolerance in rice Frontiers in Plant Science rice nucleoredoxin gene family drought stress genome-wide identification expression pattern |
| title | Genome-wide identification of the nuclear redox protein gene family revealed its potential role in drought stress tolerance in rice |
| title_full | Genome-wide identification of the nuclear redox protein gene family revealed its potential role in drought stress tolerance in rice |
| title_fullStr | Genome-wide identification of the nuclear redox protein gene family revealed its potential role in drought stress tolerance in rice |
| title_full_unstemmed | Genome-wide identification of the nuclear redox protein gene family revealed its potential role in drought stress tolerance in rice |
| title_short | Genome-wide identification of the nuclear redox protein gene family revealed its potential role in drought stress tolerance in rice |
| title_sort | genome wide identification of the nuclear redox protein gene family revealed its potential role in drought stress tolerance in rice |
| topic | rice nucleoredoxin gene family drought stress genome-wide identification expression pattern |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1562718/full |
| work_keys_str_mv | AT zijieliu genomewideidentificationofthenuclearredoxproteingenefamilyrevealeditspotentialroleindroughtstresstoleranceinrice AT xingfeizheng genomewideidentificationofthenuclearredoxproteingenefamilyrevealeditspotentialroleindroughtstresstoleranceinrice AT dabingyang genomewideidentificationofthenuclearredoxproteingenefamilyrevealeditspotentialroleindroughtstresstoleranceinrice AT lanzhili genomewideidentificationofthenuclearredoxproteingenefamilyrevealeditspotentialroleindroughtstresstoleranceinrice AT hexingyin genomewideidentificationofthenuclearredoxproteingenefamilyrevealeditspotentialroleindroughtstresstoleranceinrice |