The effect of hemodialysis therapy on serum microRNA-92a
Objective To explore the effect of hemodialysis therapy on removal of serum microRNA-92 a and its mechanism on injury of vascular endothelial cells. Methods The serum samples of patients with chronic kidney diseases in XI’AN GaoXin Hospital treated with long-term hemodialysis were collected before a...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Department of Journal of Clinical Nephrology
2019-01-01
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| Series: | Linchuang shenzangbing zazhi |
| Subjects: | |
| Online Access: | http://www.lcszb.com/thesisDetails?columnId=57913184&Fpath=home&index=0 |
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| Summary: | Objective To explore the effect of hemodialysis therapy on removal of serum microRNA-92 a and its mechanism on injury of vascular endothelial cells. Methods The serum samples of patients with chronic kidney diseases in XI’AN GaoXin Hospital treated with long-term hemodialysis were collected before and 6 h after dialysis, and serum miR-92 a level, BUN, Scr and hsCRP were measured. The cultured endothelial cells were stimulated with the serum samples from patients with chronic kidney diseases before and after dialysis, so as to observe the expression changes of miR-92 a and its target gene eNOS. Results There was no significant difference in serum miR-92 a level(1.48±0.24 vs 1.20±0.18, P=0.356) and hsCRP(7.6±2.4vs 7.5±3.1, P=0.862) before and after hemodialysis. Serum BUN(26.4±8.8 vs 8.3±2.3, P<0.01) and Scr(926.3±178.1 vs 312.3±141.2, P<0.01) levels were decreased significantly by hemodialysis therapy. The further in vitro cell assay, RT-PCR, confirmed that compared with the normal healthy human serum, the serum from chronic kidney disease patients before and after dialysis could promote expression of miR-92 a n in the cultured HUVEC(P<0.001). RT-PCR and Western Blot test demonstrated that the expression of its target gene eNOS was significantly reduced(P<0.001).Conclusions Serum miR-92 a level does not obviously decrease after hemodialysis. miR-92 a can reduce the expression of eNOS in endothelial cells, which may be part of the reason why dialysis can maintain renal function but cannot prevent against cardiovascular complications. |
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| ISSN: | 1671-2390 |