The Effect of Age on the Regenerative Potential of Human Eyelid Adipose-Derived Stem Cells
Human eyelid adipose-derived stem cells (HEASCs) are a new source of autologous mesenchymal stem cells, which are derived from neuroectoderm and potentially applied in the tissue regeneration and cell therapies. Based on the prevalence of blepharoplasty in Asia and the availability of HEASCs, we inv...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/5654917 |
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| author | Mingqi Zhang Zhuoshi Wang Yan Zhao Lirong Zhang Ling Xu Liu Cao Wei He |
| author_facet | Mingqi Zhang Zhuoshi Wang Yan Zhao Lirong Zhang Ling Xu Liu Cao Wei He |
| author_sort | Mingqi Zhang |
| collection | DOAJ |
| description | Human eyelid adipose-derived stem cells (HEASCs) are a new source of autologous mesenchymal stem cells, which are derived from neuroectoderm and potentially applied in the tissue regeneration and cell therapies. Based on the prevalence of blepharoplasty in Asia and the availability of HEASCs, we investigated the effect of donor age on their characteristics and regenerative potential of HEASCs in vitro. The HEASCs were isolated from patients of three groups: (1) <20 years (n=4), (2) >20 years, <45 years (n=5), and (3) >55 years (n=4). For each group, the proliferative capacity, colony-forming ability, surface markers, differentiation ability, wound healing function, and secreted protein were contrastively evaluated and quantified for statistical analysis. It was found that HEASCs were successfully isolated and cultured by an explant culture method. The proliferative rates, osteogenic and chondrogenic differentiation potentials, wound healing ability, and the expression of TGF-β1 and fibronectin protein of HEASCs significantly decreased as age increased. However, the expression of CD90 antigen and the adipogenic differentiation showed an age-related increase in HEASCs. As many degenerative diseases increase in prevalence with age, the age-related changes of the HEASCs proliferation potential, differentiation capacity, and wound healing ability should be taken into account whenever they are intended for use in research or cytotherapy. |
| format | Article |
| id | doaj-art-ef220a60f30c4fc08dc8f0fa18569957 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-ef220a60f30c4fc08dc8f0fa185699572025-02-03T01:21:59ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/56549175654917The Effect of Age on the Regenerative Potential of Human Eyelid Adipose-Derived Stem CellsMingqi Zhang0Zhuoshi Wang1Yan Zhao2Lirong Zhang3Ling Xu4Liu Cao5Wei He6Key Laboratory of Medical Cell Biology, Institute of Translational Medicine, China Medical University, Shenyang, Liaoning, ChinaKey Laboratory of Medical Cell Biology, Institute of Translational Medicine, China Medical University, Shenyang, Liaoning, ChinaKey laboratory of Stem Cell Research, College of Basic Medicine, He University, Shenyang, Liaoning, ChinaClinical Research Center, He Eye Hospital of He University, Shenyang, Liaoning, ChinaClinical Research Center, He Eye Hospital of He University, Shenyang, Liaoning, ChinaKey Laboratory of Medical Cell Biology, Institute of Translational Medicine, China Medical University, Shenyang, Liaoning, ChinaKey Laboratory of Medical Cell Biology, Institute of Translational Medicine, China Medical University, Shenyang, Liaoning, ChinaHuman eyelid adipose-derived stem cells (HEASCs) are a new source of autologous mesenchymal stem cells, which are derived from neuroectoderm and potentially applied in the tissue regeneration and cell therapies. Based on the prevalence of blepharoplasty in Asia and the availability of HEASCs, we investigated the effect of donor age on their characteristics and regenerative potential of HEASCs in vitro. The HEASCs were isolated from patients of three groups: (1) <20 years (n=4), (2) >20 years, <45 years (n=5), and (3) >55 years (n=4). For each group, the proliferative capacity, colony-forming ability, surface markers, differentiation ability, wound healing function, and secreted protein were contrastively evaluated and quantified for statistical analysis. It was found that HEASCs were successfully isolated and cultured by an explant culture method. The proliferative rates, osteogenic and chondrogenic differentiation potentials, wound healing ability, and the expression of TGF-β1 and fibronectin protein of HEASCs significantly decreased as age increased. However, the expression of CD90 antigen and the adipogenic differentiation showed an age-related increase in HEASCs. As many degenerative diseases increase in prevalence with age, the age-related changes of the HEASCs proliferation potential, differentiation capacity, and wound healing ability should be taken into account whenever they are intended for use in research or cytotherapy.http://dx.doi.org/10.1155/2018/5654917 |
| spellingShingle | Mingqi Zhang Zhuoshi Wang Yan Zhao Lirong Zhang Ling Xu Liu Cao Wei He The Effect of Age on the Regenerative Potential of Human Eyelid Adipose-Derived Stem Cells Stem Cells International |
| title | The Effect of Age on the Regenerative Potential of Human Eyelid Adipose-Derived Stem Cells |
| title_full | The Effect of Age on the Regenerative Potential of Human Eyelid Adipose-Derived Stem Cells |
| title_fullStr | The Effect of Age on the Regenerative Potential of Human Eyelid Adipose-Derived Stem Cells |
| title_full_unstemmed | The Effect of Age on the Regenerative Potential of Human Eyelid Adipose-Derived Stem Cells |
| title_short | The Effect of Age on the Regenerative Potential of Human Eyelid Adipose-Derived Stem Cells |
| title_sort | effect of age on the regenerative potential of human eyelid adipose derived stem cells |
| url | http://dx.doi.org/10.1155/2018/5654917 |
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