Expression of SUR1 isoforms in the brain and heart after ischemia/reperfusion

The sulfonylurea receptor 1 (SUR1) has been classified as a member of the adenosine triphosphate (ATP)-binding cassette (ABC) transporter superfamily. SUR1, unlike the classic ABC transporters, assembles with Kir6.2, forming KATP channels to regulate the flux of potassium ions. In the central nervou...

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Main Authors: Iván Alquisiras-Burgos, Irlanda Peralta-Arrieta, Mónica Espinoza-Rojo, Alejandro Salazar-Salgado, Iván Antonino-Olguín, Alicia Sánchez-Mendoza, María Sánchez-Aguilar, Martha-Eugenia Ruiz-Tachiquín, Hilda-Alicia Valdez-Salazar, Alma Ortiz-Plata, Javier Franco-Pérez, Arturo Hernández-Cruz, Penélope Aguilera
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Molecular Neuroscience
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Online Access:https://www.frontiersin.org/articles/10.3389/fnmol.2025.1536409/full
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author Iván Alquisiras-Burgos
Irlanda Peralta-Arrieta
Mónica Espinoza-Rojo
Alejandro Salazar-Salgado
Iván Antonino-Olguín
Iván Antonino-Olguín
Alicia Sánchez-Mendoza
María Sánchez-Aguilar
Martha-Eugenia Ruiz-Tachiquín
Hilda-Alicia Valdez-Salazar
Alma Ortiz-Plata
Javier Franco-Pérez
Arturo Hernández-Cruz
Penélope Aguilera
author_facet Iván Alquisiras-Burgos
Irlanda Peralta-Arrieta
Mónica Espinoza-Rojo
Alejandro Salazar-Salgado
Iván Antonino-Olguín
Iván Antonino-Olguín
Alicia Sánchez-Mendoza
María Sánchez-Aguilar
Martha-Eugenia Ruiz-Tachiquín
Hilda-Alicia Valdez-Salazar
Alma Ortiz-Plata
Javier Franco-Pérez
Arturo Hernández-Cruz
Penélope Aguilera
author_sort Iván Alquisiras-Burgos
collection DOAJ
description The sulfonylurea receptor 1 (SUR1) has been classified as a member of the adenosine triphosphate (ATP)-binding cassette (ABC) transporter superfamily. SUR1, unlike the classic ABC transporters, assembles with Kir6.2, forming KATP channels to regulate the flux of potassium ions. In the central nervous system, SUR1 is weakly expressed in some brain regions but is induced by pathological conditions in the different cell types of the neurovascular unit. Therefore, we first analyzed the expression of SUR1 in various rat tissues and brain regions to identify SUR1 isoforms and their mRNA exon composition under physiological conditions. Later, we focused on the SUR1 expression in the brain and heart after ischemia/reperfusion. We observed two SUR1 isoforms (170 and 60–75 kDa) abundantly expressed in most rat tissues, except for the testis and brain, where basal expression of these isoforms was relatively low and exhibit a band of 100 kDa. Every exons coding for the functional domains of SUR1 mRNA were amplified from the tissues and brain regions analyzed. Results from in vitro and in vivo experiments indicated that SUR1 isoforms previously identified (170 and 60–75 kDa) were dramatically overexpressed in the brain after middle cerebral artery occlusion followed by reperfusion. In contrast, myocardial infarction followed by reperfusion significantly reduced SUR1 isoform expression in the heart. This study demonstrates the expression of at least two SUR1 isoforms in various tissues and suggests that ischemic processes may differentially regulate SUR1 expression depending on the tissue injured.
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spelling doaj-art-ef1d0897275c41f4b138485a533fe36b2025-08-20T02:13:19ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992025-04-011810.3389/fnmol.2025.15364091536409Expression of SUR1 isoforms in the brain and heart after ischemia/reperfusionIván Alquisiras-Burgos0Irlanda Peralta-Arrieta1Mónica Espinoza-Rojo2Alejandro Salazar-Salgado3Iván Antonino-Olguín4Iván Antonino-Olguín5Alicia Sánchez-Mendoza6María Sánchez-Aguilar7Martha-Eugenia Ruiz-Tachiquín8Hilda-Alicia Valdez-Salazar9Alma Ortiz-Plata10Javier Franco-Pérez11Arturo Hernández-Cruz12Penélope Aguilera13Laboratorio de Patología Vascular Cerebral, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Ciudad de México, MexicoLaboratorio de Transducción de Señales, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Ciudad de México, MexicoLaboratorio de Biología Molecular y Genómica, Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Guerrero, Chilpancingo de los Bravo, MexicoDepartamento de Neuropatología Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México, MexicoLaboratorio de Patología Vascular Cerebral, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Ciudad de México, MexicoLaboratorio de Biología Molecular y Genómica, Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Guerrero, Chilpancingo de los Bravo, MexicoDepartamento de Farmacología, Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de México, MexicoDepartamento de Farmacología, Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de México, MexicoUnidad de Investigación Médica en Enfermedades Oncológicas, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México, MexicoUnidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México, MexicoLaboratorio de Patología Experimental, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Ciudad de México, MexicoLaboratorio de Patología Vascular Cerebral, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Ciudad de México, MexicoDepartamento de Neuropatología Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México, MexicoLaboratorio de Patología Vascular Cerebral, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Ciudad de México, MexicoThe sulfonylurea receptor 1 (SUR1) has been classified as a member of the adenosine triphosphate (ATP)-binding cassette (ABC) transporter superfamily. SUR1, unlike the classic ABC transporters, assembles with Kir6.2, forming KATP channels to regulate the flux of potassium ions. In the central nervous system, SUR1 is weakly expressed in some brain regions but is induced by pathological conditions in the different cell types of the neurovascular unit. Therefore, we first analyzed the expression of SUR1 in various rat tissues and brain regions to identify SUR1 isoforms and their mRNA exon composition under physiological conditions. Later, we focused on the SUR1 expression in the brain and heart after ischemia/reperfusion. We observed two SUR1 isoforms (170 and 60–75 kDa) abundantly expressed in most rat tissues, except for the testis and brain, where basal expression of these isoforms was relatively low and exhibit a band of 100 kDa. Every exons coding for the functional domains of SUR1 mRNA were amplified from the tissues and brain regions analyzed. Results from in vitro and in vivo experiments indicated that SUR1 isoforms previously identified (170 and 60–75 kDa) were dramatically overexpressed in the brain after middle cerebral artery occlusion followed by reperfusion. In contrast, myocardial infarction followed by reperfusion significantly reduced SUR1 isoform expression in the heart. This study demonstrates the expression of at least two SUR1 isoforms in various tissues and suggests that ischemic processes may differentially regulate SUR1 expression depending on the tissue injured.https://www.frontiersin.org/articles/10.3389/fnmol.2025.1536409/fullsulphonylurea 1 receptorSUR1brain ischemiaheart ischemiaAbcc8
spellingShingle Iván Alquisiras-Burgos
Irlanda Peralta-Arrieta
Mónica Espinoza-Rojo
Alejandro Salazar-Salgado
Iván Antonino-Olguín
Iván Antonino-Olguín
Alicia Sánchez-Mendoza
María Sánchez-Aguilar
Martha-Eugenia Ruiz-Tachiquín
Hilda-Alicia Valdez-Salazar
Alma Ortiz-Plata
Javier Franco-Pérez
Arturo Hernández-Cruz
Penélope Aguilera
Expression of SUR1 isoforms in the brain and heart after ischemia/reperfusion
Frontiers in Molecular Neuroscience
sulphonylurea 1 receptor
SUR1
brain ischemia
heart ischemia
Abcc8
title Expression of SUR1 isoforms in the brain and heart after ischemia/reperfusion
title_full Expression of SUR1 isoforms in the brain and heart after ischemia/reperfusion
title_fullStr Expression of SUR1 isoforms in the brain and heart after ischemia/reperfusion
title_full_unstemmed Expression of SUR1 isoforms in the brain and heart after ischemia/reperfusion
title_short Expression of SUR1 isoforms in the brain and heart after ischemia/reperfusion
title_sort expression of sur1 isoforms in the brain and heart after ischemia reperfusion
topic sulphonylurea 1 receptor
SUR1
brain ischemia
heart ischemia
Abcc8
url https://www.frontiersin.org/articles/10.3389/fnmol.2025.1536409/full
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