Generation of a homozygous TIGIT gene knockout (TIGIT−/−) human iPSC line (MUSIi001-A-3) using CRISPR/Cas9 system
Adoptive cell therapy for solid cancers involves enhancing and reinfusing immune cells to target tumor cells. The advancement of induced pluripotent stem cell technology enables the generation of immune cell products like T and NK cells for ACT. However, the expression of inhibitory receptors, such...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187350612400299X |
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| author | Jakkrapatra Srisantitham Siriwal Suwanpitak Nontaphat Thongsin Methichit Wattanapanitch |
| author_facet | Jakkrapatra Srisantitham Siriwal Suwanpitak Nontaphat Thongsin Methichit Wattanapanitch |
| author_sort | Jakkrapatra Srisantitham |
| collection | DOAJ |
| description | Adoptive cell therapy for solid cancers involves enhancing and reinfusing immune cells to target tumor cells. The advancement of induced pluripotent stem cell technology enables the generation of immune cell products like T and NK cells for ACT. However, the expression of inhibitory receptors, such as TIGIT, may limit the functionality of these immune effector cells. In this study, we generated a homozygous TIGIT gene knockout iPSC line to potentially prevent inhibitory signaling and exhaustion, thereby creating potent “off-the-shelf” immune cell products for cellular immunotherapy applications. This approach could offer a new frontier in the fight against solid tumors. |
| format | Article |
| id | doaj-art-ef1cc8381e4e449d8764591bcb230c61 |
| institution | OA Journals |
| issn | 1873-5061 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-ef1cc8381e4e449d8764591bcb230c612025-08-20T02:38:17ZengElsevierStem Cell Research1873-50612024-12-018110360110.1016/j.scr.2024.103601Generation of a homozygous TIGIT gene knockout (TIGIT−/−) human iPSC line (MUSIi001-A-3) using CRISPR/Cas9 systemJakkrapatra Srisantitham0Siriwal Suwanpitak1Nontaphat Thongsin2Methichit Wattanapanitch3Siriraj Center for Regenerative Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Center for Regenerative Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Center for Regenerative Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Center for Regenerative Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Corresponding author.Adoptive cell therapy for solid cancers involves enhancing and reinfusing immune cells to target tumor cells. The advancement of induced pluripotent stem cell technology enables the generation of immune cell products like T and NK cells for ACT. However, the expression of inhibitory receptors, such as TIGIT, may limit the functionality of these immune effector cells. In this study, we generated a homozygous TIGIT gene knockout iPSC line to potentially prevent inhibitory signaling and exhaustion, thereby creating potent “off-the-shelf” immune cell products for cellular immunotherapy applications. This approach could offer a new frontier in the fight against solid tumors.http://www.sciencedirect.com/science/article/pii/S187350612400299X |
| spellingShingle | Jakkrapatra Srisantitham Siriwal Suwanpitak Nontaphat Thongsin Methichit Wattanapanitch Generation of a homozygous TIGIT gene knockout (TIGIT−/−) human iPSC line (MUSIi001-A-3) using CRISPR/Cas9 system Stem Cell Research |
| title | Generation of a homozygous TIGIT gene knockout (TIGIT−/−) human iPSC line (MUSIi001-A-3) using CRISPR/Cas9 system |
| title_full | Generation of a homozygous TIGIT gene knockout (TIGIT−/−) human iPSC line (MUSIi001-A-3) using CRISPR/Cas9 system |
| title_fullStr | Generation of a homozygous TIGIT gene knockout (TIGIT−/−) human iPSC line (MUSIi001-A-3) using CRISPR/Cas9 system |
| title_full_unstemmed | Generation of a homozygous TIGIT gene knockout (TIGIT−/−) human iPSC line (MUSIi001-A-3) using CRISPR/Cas9 system |
| title_short | Generation of a homozygous TIGIT gene knockout (TIGIT−/−) human iPSC line (MUSIi001-A-3) using CRISPR/Cas9 system |
| title_sort | generation of a homozygous tigit gene knockout tigit human ipsc line musii001 a 3 using crispr cas9 system |
| url | http://www.sciencedirect.com/science/article/pii/S187350612400299X |
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