Utilization of ursolic acid methyl ester in the treatment of Streptococcus pyogenes in Nile tilapia, concerning physiological, genetic, immune-oxidative, stress, and neuro-ethological parameters
The present work is an innovative approach for application the Streptomyces misakiensis S. misakiensis metabolite called ursolic acid methyl ester (UAME) in treating Streptococcus pyogenes of Nile tilapia. A total of 110 fish was used for assessment of the therapeutic dose of UAME. In addition, the...
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2025-07-01
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| author | Rewan Abdelaziz Mona Abd El khalek Salem Walaa El-Houseiny Abdelwahab A. Abdelwarith Elsayed M. Younis Heba H. Mahboub Shimaa Zayed Reham A. Abd El-Wahab Basma A. Elshafey Simon J. Davies Amany Omar Selim |
| author_facet | Rewan Abdelaziz Mona Abd El khalek Salem Walaa El-Houseiny Abdelwahab A. Abdelwarith Elsayed M. Younis Heba H. Mahboub Shimaa Zayed Reham A. Abd El-Wahab Basma A. Elshafey Simon J. Davies Amany Omar Selim |
| author_sort | Rewan Abdelaziz |
| collection | DOAJ |
| description | The present work is an innovative approach for application the Streptomyces misakiensis S. misakiensis metabolite called ursolic acid methyl ester (UAME) in treating Streptococcus pyogenes of Nile tilapia. A total of 110 fish was used for assessment of the therapeutic dose of UAME. In addition, the therapeutic efficacy of UAME was tested in vivo against S. pyogenes infection (after determination of LC50) using 200 Nile tilapia. Fish (30.00 ± 1.5 g) were divided into four groups: CON (control), UAME (1.0 mg/L), S. pyogenes, and UAME (1.0 mg/L) + S. pyogenes groups. The inoculations of 0.2 mL of S. pyogenes (1 ×107 CFU/mL) were administered to the fish in the S. pyogenes and UAME + S. pyogenes groups and continued for ten days. The minimal inhibitory concentration of UAME was 0.5 µg/mL. Moreover, irregularity and bacterial cell shape distortion were evident after treatment with UAME under a scanning Electron Microscope. Challenged groups in S. pyogenes exhibited significant increases in surfacing frequency, swimming, laterality and aggression behavior (P < 0.05). Along with this, the hepatorenal metrics (ALT, ALP, AST, creatinine, total bilirubin, and urea.), MDA (an oxidant biomarker), (8-OHdG), glucose and cortisol (stress indicators) were all significantly elevated in the diseased fish (P < 0.05). Contrariwise, the infected fish showed a significant drop (P < 0.05) in survival %, red blood cells, hemoglobin, total proteins, total globulin, albumin, AchE activity, and the immune-antioxidant indices (IgM, complement factor 3, lysozyme activity, nitric oxide, hepatic CAT, SOD, GSH, and GPx). Analysis of genes in response to S. pyogenes infection demonstrated an up-regulation of the pro/anti-inflammatory genes (il1b, tnfa, il8, tgfb, il10). Surprisingly, enriched groups in UAME modulated all the measured parameters. Overall, UAME is a promising antibacterial candidate that can efficiently retrieve stress and enhance behavioral, biochemical, immune-antioxidant, and genetic changes endowed by S. pyogenes infection. |
| format | Article |
| id | doaj-art-ef1c31db0f4c46c09d4f2e5277b0cc3f |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
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| spelling | doaj-art-ef1c31db0f4c46c09d4f2e5277b0cc3f2025-08-20T03:45:47ZengElsevierAquaculture Reports2352-51342025-07-014210277610.1016/j.aqrep.2025.102776Utilization of ursolic acid methyl ester in the treatment of Streptococcus pyogenes in Nile tilapia, concerning physiological, genetic, immune-oxidative, stress, and neuro-ethological parametersRewan Abdelaziz0Mona Abd El khalek Salem1Walaa El-Houseiny2Abdelwahab A. Abdelwarith3Elsayed M. Younis4Heba H. Mahboub5Shimaa Zayed6Reham A. Abd El-Wahab7Basma A. Elshafey8Simon J. Davies9Amany Omar Selim10Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, EgyptDepartment of Fish Disease, Animal Health Research Institute (AHRI) (Mansoura branch) Agriculture Research Center (ARC), P.O. Box 246 Dokki, Giza 12618, EgyptDepartment of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Sharkia, Egypt; Corresponding authors.Department of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Sharkia, Egypt; Corresponding authors.Biochemistry, Nutritional Deficiency unit, Animal Health Research Institute (AHRI) (Mansoura branch) Agriculture Research Center (ARC), P.O. Box 246 Dokki, Giza 12618, EgyptBiochemistry, Nutritional Deficiency unit, Animal Health Research Institute (AHRI) (Mansoura branch) Agriculture Research Center (ARC), P.O. Box 246 Dokki, Giza 12618, EgyptDepartment of Husbandary and Animal Wealth Development, Faculty of Veterinary Medicine, Sadat city University, Sadat City, EgyptAquaculture Nutrition Research Unit ANRU, Carna Research Station, Ryan Institute, College of Science and Engineering, University of Galway, Galway H91V8Y1, IrelandMicrobiology, Animal Health Research Institute (AHRI) (Banha branch) Agriculture Research Center (ARC), P.O. Box 246 Dokki, Giza 12618, EgyptThe present work is an innovative approach for application the Streptomyces misakiensis S. misakiensis metabolite called ursolic acid methyl ester (UAME) in treating Streptococcus pyogenes of Nile tilapia. A total of 110 fish was used for assessment of the therapeutic dose of UAME. In addition, the therapeutic efficacy of UAME was tested in vivo against S. pyogenes infection (after determination of LC50) using 200 Nile tilapia. Fish (30.00 ± 1.5 g) were divided into four groups: CON (control), UAME (1.0 mg/L), S. pyogenes, and UAME (1.0 mg/L) + S. pyogenes groups. The inoculations of 0.2 mL of S. pyogenes (1 ×107 CFU/mL) were administered to the fish in the S. pyogenes and UAME + S. pyogenes groups and continued for ten days. The minimal inhibitory concentration of UAME was 0.5 µg/mL. Moreover, irregularity and bacterial cell shape distortion were evident after treatment with UAME under a scanning Electron Microscope. Challenged groups in S. pyogenes exhibited significant increases in surfacing frequency, swimming, laterality and aggression behavior (P < 0.05). Along with this, the hepatorenal metrics (ALT, ALP, AST, creatinine, total bilirubin, and urea.), MDA (an oxidant biomarker), (8-OHdG), glucose and cortisol (stress indicators) were all significantly elevated in the diseased fish (P < 0.05). Contrariwise, the infected fish showed a significant drop (P < 0.05) in survival %, red blood cells, hemoglobin, total proteins, total globulin, albumin, AchE activity, and the immune-antioxidant indices (IgM, complement factor 3, lysozyme activity, nitric oxide, hepatic CAT, SOD, GSH, and GPx). Analysis of genes in response to S. pyogenes infection demonstrated an up-regulation of the pro/anti-inflammatory genes (il1b, tnfa, il8, tgfb, il10). Surprisingly, enriched groups in UAME modulated all the measured parameters. Overall, UAME is a promising antibacterial candidate that can efficiently retrieve stress and enhance behavioral, biochemical, immune-antioxidant, and genetic changes endowed by S. pyogenes infection.http://www.sciencedirect.com/science/article/pii/S2352513425001620Streptomyces misakiensisUrsolic acid methyl esterStreptococcus pyogenesNile tilapiaBiochemical-genetic analysis |
| spellingShingle | Rewan Abdelaziz Mona Abd El khalek Salem Walaa El-Houseiny Abdelwahab A. Abdelwarith Elsayed M. Younis Heba H. Mahboub Shimaa Zayed Reham A. Abd El-Wahab Basma A. Elshafey Simon J. Davies Amany Omar Selim Utilization of ursolic acid methyl ester in the treatment of Streptococcus pyogenes in Nile tilapia, concerning physiological, genetic, immune-oxidative, stress, and neuro-ethological parameters Aquaculture Reports Streptomyces misakiensis Ursolic acid methyl ester Streptococcus pyogenes Nile tilapia Biochemical-genetic analysis |
| title | Utilization of ursolic acid methyl ester in the treatment of Streptococcus pyogenes in Nile tilapia, concerning physiological, genetic, immune-oxidative, stress, and neuro-ethological parameters |
| title_full | Utilization of ursolic acid methyl ester in the treatment of Streptococcus pyogenes in Nile tilapia, concerning physiological, genetic, immune-oxidative, stress, and neuro-ethological parameters |
| title_fullStr | Utilization of ursolic acid methyl ester in the treatment of Streptococcus pyogenes in Nile tilapia, concerning physiological, genetic, immune-oxidative, stress, and neuro-ethological parameters |
| title_full_unstemmed | Utilization of ursolic acid methyl ester in the treatment of Streptococcus pyogenes in Nile tilapia, concerning physiological, genetic, immune-oxidative, stress, and neuro-ethological parameters |
| title_short | Utilization of ursolic acid methyl ester in the treatment of Streptococcus pyogenes in Nile tilapia, concerning physiological, genetic, immune-oxidative, stress, and neuro-ethological parameters |
| title_sort | utilization of ursolic acid methyl ester in the treatment of streptococcus pyogenes in nile tilapia concerning physiological genetic immune oxidative stress and neuro ethological parameters |
| topic | Streptomyces misakiensis Ursolic acid methyl ester Streptococcus pyogenes Nile tilapia Biochemical-genetic analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425001620 |
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