The clinic application of mNGS and ENA-78 assays to identify intra-amniotic infection/inflammation
ObjectiveThe objective of this study is to explore whether metagenomic next-generation sequencing (mNGS) and Epithelial Neutrophil Activating Peptide-78 (ENA-78) assays in the amniotic fluid (AF) of patients with preterm labor (PTL) could be employed for diagnosing intra-amniotic infection/inflammat...
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1510671/full |
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| author | Di Shen Di Shen Hui Ju Hongying Wang Xietong Wang Xietong Wang Guangzhen Li |
| author_facet | Di Shen Di Shen Hui Ju Hongying Wang Xietong Wang Xietong Wang Guangzhen Li |
| author_sort | Di Shen |
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| description | ObjectiveThe objective of this study is to explore whether metagenomic next-generation sequencing (mNGS) and Epithelial Neutrophil Activating Peptide-78 (ENA-78) assays in the amniotic fluid (AF) of patients with preterm labor (PTL) could be employed for diagnosing intra-amniotic infection/inflammation (IAI/I) and predict the outcomes of emergency cerclage in women with cervical insufficiency(CI).MethodsAF samples from 40 patients were subjected to PTL were subjected to mNGS and microbial culture to diagnose intra-amniotic infection known as microbial invasion of the amniotic cavity (MIAC); ELISA was used to analyze ENA-78 levels for prediction of intra-amniotic inflammation (IAI). Pregnancy outcomes were compared, the predictive performance of mNGS and ENA-78 were assessed to evaluate the efficacy of emergency cervical cerclage.ResultsThe diagnosis rate of MIAC was higher with mNGS (17.5%) compared to microbial culture (2.5%). AF ENA-78 levels were significantly higher in IAI patients than in non-IAI/I patients. ENA-78 demonstrated certain accuracy in identifying IAI, with sensitivity and specificity of 73.3% and 100%, respectively. Compared with non-IAI/I patients, patients with MIAC or IAI exhibited poor pregnancy outcomes after cervical cerclage.ConclusionsmNGS and ENA-78 assays are valuable means for assessing the state of infection/inflammation in the amniotic cavity and predicting the outcomes of emergency cerclage. |
| format | Article |
| id | doaj-art-ef16e2faf0ad499285e74949373b3639 |
| institution | OA Journals |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-ef16e2faf0ad499285e74949373b36392025-08-20T01:51:42ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-04-011510.3389/fcimb.2025.15106711510671The clinic application of mNGS and ENA-78 assays to identify intra-amniotic infection/inflammationDi Shen0Di Shen1Hui Ju2Hongying Wang3Xietong Wang4Xietong Wang5Guangzhen Li6Department of Obstetrics and Gynaecology, Key Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Shandong Provincial Maternal and Child Health Care Hospital Affiliated to Qingdao University, Jinan, ChinaDepartment of Obstetrics and Gynecology, Liao Cheng People’s Hospital, Liaocheng, ChinaDepartment of Obstetrics and Gynaecology, Key Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Shandong Provincial Maternal and Child Health Care Hospital Affiliated to Qingdao University, Jinan, ChinaDepartment of Obstetrics and Gynecology, Liao Cheng People’s Hospital, Liaocheng, ChinaDepartment of Obstetrics and Gynaecology, Key Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Shandong Provincial Maternal and Child Health Care Hospital Affiliated to Qingdao University, Jinan, ChinaDepartment of Obstetrics and Gynaecology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, ChinaDepartment of General Surgery, Qilu Hospital of Shandong University, Jinan, ChinaObjectiveThe objective of this study is to explore whether metagenomic next-generation sequencing (mNGS) and Epithelial Neutrophil Activating Peptide-78 (ENA-78) assays in the amniotic fluid (AF) of patients with preterm labor (PTL) could be employed for diagnosing intra-amniotic infection/inflammation (IAI/I) and predict the outcomes of emergency cerclage in women with cervical insufficiency(CI).MethodsAF samples from 40 patients were subjected to PTL were subjected to mNGS and microbial culture to diagnose intra-amniotic infection known as microbial invasion of the amniotic cavity (MIAC); ELISA was used to analyze ENA-78 levels for prediction of intra-amniotic inflammation (IAI). Pregnancy outcomes were compared, the predictive performance of mNGS and ENA-78 were assessed to evaluate the efficacy of emergency cervical cerclage.ResultsThe diagnosis rate of MIAC was higher with mNGS (17.5%) compared to microbial culture (2.5%). AF ENA-78 levels were significantly higher in IAI patients than in non-IAI/I patients. ENA-78 demonstrated certain accuracy in identifying IAI, with sensitivity and specificity of 73.3% and 100%, respectively. Compared with non-IAI/I patients, patients with MIAC or IAI exhibited poor pregnancy outcomes after cervical cerclage.ConclusionsmNGS and ENA-78 assays are valuable means for assessing the state of infection/inflammation in the amniotic cavity and predicting the outcomes of emergency cerclage.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1510671/fullpreterm laborintra-amniotic infection/inflammationmetagenomic next-generation sequencingneutrophil extracellular trapscervical insufficiency |
| spellingShingle | Di Shen Di Shen Hui Ju Hongying Wang Xietong Wang Xietong Wang Guangzhen Li The clinic application of mNGS and ENA-78 assays to identify intra-amniotic infection/inflammation Frontiers in Cellular and Infection Microbiology preterm labor intra-amniotic infection/inflammation metagenomic next-generation sequencing neutrophil extracellular traps cervical insufficiency |
| title | The clinic application of mNGS and ENA-78 assays to identify intra-amniotic infection/inflammation |
| title_full | The clinic application of mNGS and ENA-78 assays to identify intra-amniotic infection/inflammation |
| title_fullStr | The clinic application of mNGS and ENA-78 assays to identify intra-amniotic infection/inflammation |
| title_full_unstemmed | The clinic application of mNGS and ENA-78 assays to identify intra-amniotic infection/inflammation |
| title_short | The clinic application of mNGS and ENA-78 assays to identify intra-amniotic infection/inflammation |
| title_sort | clinic application of mngs and ena 78 assays to identify intra amniotic infection inflammation |
| topic | preterm labor intra-amniotic infection/inflammation metagenomic next-generation sequencing neutrophil extracellular traps cervical insufficiency |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1510671/full |
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