Strain-specific galactose utilization by commensal E. coli mitigates Salmonella establishment in the gut.
Salmonella enterica serovar Typhimurium (S. Tm) is a major cause of gastrointestinal diseases worldwide. To date, options for prevention or curative therapy remain limited. The gut microbiota plays a protective role against enteric diseases, particularly in preventing establishment and proliferation...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-06-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1013232 |
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| author | Christopher Schubert Jana Näf Lisa Petukhov Leanid Laganenka Yassine Cherrak Wolf-Dietrich Hardt |
| author_facet | Christopher Schubert Jana Näf Lisa Petukhov Leanid Laganenka Yassine Cherrak Wolf-Dietrich Hardt |
| author_sort | Christopher Schubert |
| collection | DOAJ |
| description | Salmonella enterica serovar Typhimurium (S. Tm) is a major cause of gastrointestinal diseases worldwide. To date, options for prevention or curative therapy remain limited. The gut microbiota plays a protective role against enteric diseases, particularly in preventing establishment and proliferation of S. Tm. While most research has focused on microbiota-mediated pathogen exclusion during the later, inflammation-dominated stages of infection, little is known about how microbiota members mitigate S. Tm early gut colonization. To address this gap, we conducted 24 h in vivo competitive experiments using S. Tm and different commensal E. coli strains. We observed a significant reduction in pathogen load, which was strain-specific and particularly evident with E. coli 8178. To investigate the underlying molecular mechanisms, we performed an in vivo screen using a rationally designed S. Tm library-which includes a wide range of carbohydrate utilization mutants-both in the absence and presence of E. coli strains. Our findings revealed that E. coli 8178-mediated S. Tm competition was driven by the exploitation of galactose during the early stage of infection. Identifying galactose as a key metabolite in pathogen exclusion by gut microbiota members enhances our mechanistic understanding of microbiota-mediated protection and opens new avenues for developing microbiota- and dietary-based strategies to better control intestinal infections. |
| format | Article |
| id | doaj-art-ef14b786abb047b6a07a4608084274d1 |
| institution | OA Journals |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-ef14b786abb047b6a07a4608084274d12025-08-20T02:36:20ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742025-06-01216e101323210.1371/journal.ppat.1013232Strain-specific galactose utilization by commensal E. coli mitigates Salmonella establishment in the gut.Christopher SchubertJana NäfLisa PetukhovLeanid LaganenkaYassine CherrakWolf-Dietrich HardtSalmonella enterica serovar Typhimurium (S. Tm) is a major cause of gastrointestinal diseases worldwide. To date, options for prevention or curative therapy remain limited. The gut microbiota plays a protective role against enteric diseases, particularly in preventing establishment and proliferation of S. Tm. While most research has focused on microbiota-mediated pathogen exclusion during the later, inflammation-dominated stages of infection, little is known about how microbiota members mitigate S. Tm early gut colonization. To address this gap, we conducted 24 h in vivo competitive experiments using S. Tm and different commensal E. coli strains. We observed a significant reduction in pathogen load, which was strain-specific and particularly evident with E. coli 8178. To investigate the underlying molecular mechanisms, we performed an in vivo screen using a rationally designed S. Tm library-which includes a wide range of carbohydrate utilization mutants-both in the absence and presence of E. coli strains. Our findings revealed that E. coli 8178-mediated S. Tm competition was driven by the exploitation of galactose during the early stage of infection. Identifying galactose as a key metabolite in pathogen exclusion by gut microbiota members enhances our mechanistic understanding of microbiota-mediated protection and opens new avenues for developing microbiota- and dietary-based strategies to better control intestinal infections.https://doi.org/10.1371/journal.ppat.1013232 |
| spellingShingle | Christopher Schubert Jana Näf Lisa Petukhov Leanid Laganenka Yassine Cherrak Wolf-Dietrich Hardt Strain-specific galactose utilization by commensal E. coli mitigates Salmonella establishment in the gut. PLoS Pathogens |
| title | Strain-specific galactose utilization by commensal E. coli mitigates Salmonella establishment in the gut. |
| title_full | Strain-specific galactose utilization by commensal E. coli mitigates Salmonella establishment in the gut. |
| title_fullStr | Strain-specific galactose utilization by commensal E. coli mitigates Salmonella establishment in the gut. |
| title_full_unstemmed | Strain-specific galactose utilization by commensal E. coli mitigates Salmonella establishment in the gut. |
| title_short | Strain-specific galactose utilization by commensal E. coli mitigates Salmonella establishment in the gut. |
| title_sort | strain specific galactose utilization by commensal e coli mitigates salmonella establishment in the gut |
| url | https://doi.org/10.1371/journal.ppat.1013232 |
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