Chemotactic Responses of <i>Oscheius myriophilus</i> to Mollusk Mucus

Terrestrial slugs and snails can significantly harm agriculture. Due to environmental concerns associated with chemical molluscicides, biological control methods are increasingly being explored. <i>Oscheius myriophilus</i> (Poinar, 1986), a nematode species recently discovered in associa...

Full description

Saved in:
Bibliographic Details
Main Authors: Žiga Laznik, Stanislav Trdan, Klara Šavli
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Agronomy
Subjects:
Online Access:https://www.mdpi.com/2073-4395/14/12/3049
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850042088900526080
author Žiga Laznik
Stanislav Trdan
Klara Šavli
author_facet Žiga Laznik
Stanislav Trdan
Klara Šavli
author_sort Žiga Laznik
collection DOAJ
description Terrestrial slugs and snails can significantly harm agriculture. Due to environmental concerns associated with chemical molluscicides, biological control methods are increasingly being explored. <i>Oscheius myriophilus</i> (Poinar, 1986), a nematode species recently discovered in association with <i>Arion vulgaris</i> Moquin-Tandon, 1855, holds promise as a biocontrol agent for gastropod pests. In this study, we investigated the chemotactic response and motility of <i>O. myriophilus</i> when exposed to the mucus of five mollusk species: <i>Helix pomatia</i> Linnaeus, 1758, <i>Cernuella virgata</i> (Da Costa, 1778), <i>Deroceras reticulatum</i> Müller, 1774, <i>A. vulgaris</i>, and <i>Tandonia budapestensis</i> Hazay, 1880. Our experiments were conducted at two temperatures (20 °C and 25 °C) to assess how environmental conditions influence nematode behavior. The results demonstrated that the chemoattractiveness of mollusk mucus to <i>O. myriophilus</i> was significantly influenced by both the species of mollusk and the temperature. Overall, nematode motility was higher at 20 °C than at 25 °C, indicating that lower temperatures may enhance the activity of <i>O. myriophilus</i>. Among the tested mollusk species, <i>C. virgata</i> mucus consistently attracted the highest number of nematodes, especially at the lower temperature. Our findings indicate that the chemotactic response of <i>O. myriophilus</i> to mollusk mucus may have potential for the targeted biocontrol of pest mollusks. While <i>C. virgata</i> demonstrated strong attractant potential at the tested temperatures, particularly under cooler conditions (20 °C), further research is needed to confirm whether this represents a consistent temperature-related effect. Future studies should aim to identify the specific chemical cues in mollusk mucus that trigger nematode attraction and examine how these signals interact with a broader range of environmental variables, including temperature, to influence nematode behavior.
format Article
id doaj-art-ef0fe5fcef8d4d87bb1a09e184850593
institution DOAJ
issn 2073-4395
language English
publishDate 2024-12-01
publisher MDPI AG
record_format Article
series Agronomy
spelling doaj-art-ef0fe5fcef8d4d87bb1a09e1848505932025-08-20T02:55:38ZengMDPI AGAgronomy2073-43952024-12-011412304910.3390/agronomy14123049Chemotactic Responses of <i>Oscheius myriophilus</i> to Mollusk MucusŽiga Laznik0Stanislav Trdan1Klara Šavli2Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaDepartment of Agronomy, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaAgricultural Institute of Slovenia, Hacquetova Ulica 17, SI-1000 Ljubljana, SloveniaTerrestrial slugs and snails can significantly harm agriculture. Due to environmental concerns associated with chemical molluscicides, biological control methods are increasingly being explored. <i>Oscheius myriophilus</i> (Poinar, 1986), a nematode species recently discovered in association with <i>Arion vulgaris</i> Moquin-Tandon, 1855, holds promise as a biocontrol agent for gastropod pests. In this study, we investigated the chemotactic response and motility of <i>O. myriophilus</i> when exposed to the mucus of five mollusk species: <i>Helix pomatia</i> Linnaeus, 1758, <i>Cernuella virgata</i> (Da Costa, 1778), <i>Deroceras reticulatum</i> Müller, 1774, <i>A. vulgaris</i>, and <i>Tandonia budapestensis</i> Hazay, 1880. Our experiments were conducted at two temperatures (20 °C and 25 °C) to assess how environmental conditions influence nematode behavior. The results demonstrated that the chemoattractiveness of mollusk mucus to <i>O. myriophilus</i> was significantly influenced by both the species of mollusk and the temperature. Overall, nematode motility was higher at 20 °C than at 25 °C, indicating that lower temperatures may enhance the activity of <i>O. myriophilus</i>. Among the tested mollusk species, <i>C. virgata</i> mucus consistently attracted the highest number of nematodes, especially at the lower temperature. Our findings indicate that the chemotactic response of <i>O. myriophilus</i> to mollusk mucus may have potential for the targeted biocontrol of pest mollusks. While <i>C. virgata</i> demonstrated strong attractant potential at the tested temperatures, particularly under cooler conditions (20 °C), further research is needed to confirm whether this represents a consistent temperature-related effect. Future studies should aim to identify the specific chemical cues in mollusk mucus that trigger nematode attraction and examine how these signals interact with a broader range of environmental variables, including temperature, to influence nematode behavior.https://www.mdpi.com/2073-4395/14/12/3049<i>Oscheius myriophilus</i>chemotaxis behaviorSpanish sluggray field slugRoman snailcommon white snail
spellingShingle Žiga Laznik
Stanislav Trdan
Klara Šavli
Chemotactic Responses of <i>Oscheius myriophilus</i> to Mollusk Mucus
Agronomy
<i>Oscheius myriophilus</i>
chemotaxis behavior
Spanish slug
gray field slug
Roman snail
common white snail
title Chemotactic Responses of <i>Oscheius myriophilus</i> to Mollusk Mucus
title_full Chemotactic Responses of <i>Oscheius myriophilus</i> to Mollusk Mucus
title_fullStr Chemotactic Responses of <i>Oscheius myriophilus</i> to Mollusk Mucus
title_full_unstemmed Chemotactic Responses of <i>Oscheius myriophilus</i> to Mollusk Mucus
title_short Chemotactic Responses of <i>Oscheius myriophilus</i> to Mollusk Mucus
title_sort chemotactic responses of i oscheius myriophilus i to mollusk mucus
topic <i>Oscheius myriophilus</i>
chemotaxis behavior
Spanish slug
gray field slug
Roman snail
common white snail
url https://www.mdpi.com/2073-4395/14/12/3049
work_keys_str_mv AT zigalaznik chemotacticresponsesofioscheiusmyriophilusitomolluskmucus
AT stanislavtrdan chemotacticresponsesofioscheiusmyriophilusitomolluskmucus
AT klarasavli chemotacticresponsesofioscheiusmyriophilusitomolluskmucus