A targeted tiled amplicon sequencing approach for clade and subclade level differentiation of monkeypox virus from wastewater

A targeted tiled amplicon sequencing approach for clade and subclade level differentiation of monkeypox virus from wastewater

Abstract Wastewater-based surveillance (WBS) has modernized in recent years and emerged as an important tool for the monitoring of viral pathogens, including monkeypox virus (MPXV). Here we describe a novel targeted amplicon sequencing method developed for clade and subclade characterization of MPXV...

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Bibliographic Details
Main Authors: Mathew Fisher, Jennifer Ali, Shayna Giesbrecht, Raveena Roopra, Veronica Carroll, Amber Papineau, Cody Buchanan, Nicholas Duan, Chrystal Landgraff
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
Subjects:
Tiled amplicon sequencing
MPOX
Wastewater
Monkeypox virus
Online Access:https://doi.org/10.1038/s41598-025-13927-y
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