Dynamic changes of extracellular vesicles during zebrafish organogenesis
Abstract Extracellular Vesicles (EVs) play a crucial role in cell differentiation. Despite its role as a well-established vertebrate model, little is known about EVs during zebrafish embryogenesis. This study investigates EVs during zebrafish embryogenesis, analysing size- and concentration-changes...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2025-02-01
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| Series: | Cell Communication and Signaling |
| Online Access: | https://doi.org/10.1186/s12964-025-02053-x |
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| author | Linda-Marie Mulzer Tim Felger Luis E. Muñoz Gesa Engl Heiko Reutter Mario Schiffer Leila Pourtalebi Jahromi Fanni Annamária Boros Friederike Zunke Philipp Arnold Alina C. Hilger |
| author_facet | Linda-Marie Mulzer Tim Felger Luis E. Muñoz Gesa Engl Heiko Reutter Mario Schiffer Leila Pourtalebi Jahromi Fanni Annamária Boros Friederike Zunke Philipp Arnold Alina C. Hilger |
| author_sort | Linda-Marie Mulzer |
| collection | DOAJ |
| description | Abstract Extracellular Vesicles (EVs) play a crucial role in cell differentiation. Despite its role as a well-established vertebrate model, little is known about EVs during zebrafish embryogenesis. This study investigates EVs during zebrafish embryogenesis, analysing size- and concentration-changes over time. Wild-type AB strain zebrafish larvae (zfl) were collected at 24, 48, 72, and 96 h post fertilization (hpf) and homogenized. EVs were isolated and characterized using flow cytometry, negative staining transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and Western Blot. Flow cytometry and TEM showed a high purity of the samples. Small EVs (sEVs) and large EVs (lEVs) were differentiated using NTA, showing that zfl use different types of EVs during embryogenesis. It was observed that the total EV number increased significantly over the first 72 hpf, but not proportionally to zfl growth in length. Additionally, sEV size also increased significantly, with a maximum diameter at 72 hpf. Since most organs are formed during the first 72 hpf and from then on mainly maturation and growth occur, the elevated number and larger size before 72 hpf suggests an important role of EVs during zebrafish organogenesis. Since EVs serve as cargo delivery platforms, the increase in size of sEVs may reflect the need for a higher transport capacity. |
| format | Article |
| id | doaj-art-ef02f347753c42809c653bad9d219933 |
| institution | Kabale University |
| issn | 1478-811X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell Communication and Signaling |
| spelling | doaj-art-ef02f347753c42809c653bad9d2199332025-02-09T12:47:19ZengBMCCell Communication and Signaling1478-811X2025-02-0123111010.1186/s12964-025-02053-xDynamic changes of extracellular vesicles during zebrafish organogenesisLinda-Marie Mulzer0Tim Felger1Luis E. Muñoz2Gesa Engl3Heiko Reutter4Mario Schiffer5Leila Pourtalebi Jahromi6Fanni Annamária Boros7Friederike Zunke8Philipp Arnold9Alina C. Hilger10Department of Pediatrics and Adolescent Medicine, Division of Neonatology and Pediatric, Intensive Care University Hospital ErlangenDepartment of Pediatrics and Adolescent Medicine, Division of Neonatology and Pediatric, Intensive Care University Hospital ErlangenDepartment of Internal Medicine 3 Rheumatology and Immunology, Friedrich-Alexander- University Erlangen-Nürnberg (FAU) and Universitätsklinikum ErlangenDepartment of Pediatrics and Adolescent Medicine, Division of Neonatology and Pediatric, Intensive Care University Hospital ErlangenDepartment of Pediatrics and Adolescent Medicine, Division of Neonatology and Pediatric, Intensive Care University Hospital ErlangenDepartment of Internal Medicine 4 Nephrology, Friedrich-Alexander-University Erlangen- Nürnberg (FAU) and Universitätsklinikum ErlangenDepartment of Biology, Friedrich-Alexander-University Erlangen (FAU)Department of Molecular Neurology, University Hospital Erlangen, Friedrich-Alexander- University Erlangen-Nürnberg (FAU)Department of Molecular Neurology, University Hospital Erlangen, Friedrich-Alexander- University Erlangen-Nürnberg (FAU)Institute of Anatomy, Functional and Clinical Anatomy, Friedrich-Alexander-University Erlangen-Nürnberg (FAU)Department of Pediatrics and Adolescent Medicine, University Hospital ErlangenAbstract Extracellular Vesicles (EVs) play a crucial role in cell differentiation. Despite its role as a well-established vertebrate model, little is known about EVs during zebrafish embryogenesis. This study investigates EVs during zebrafish embryogenesis, analysing size- and concentration-changes over time. Wild-type AB strain zebrafish larvae (zfl) were collected at 24, 48, 72, and 96 h post fertilization (hpf) and homogenized. EVs were isolated and characterized using flow cytometry, negative staining transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and Western Blot. Flow cytometry and TEM showed a high purity of the samples. Small EVs (sEVs) and large EVs (lEVs) were differentiated using NTA, showing that zfl use different types of EVs during embryogenesis. It was observed that the total EV number increased significantly over the first 72 hpf, but not proportionally to zfl growth in length. Additionally, sEV size also increased significantly, with a maximum diameter at 72 hpf. Since most organs are formed during the first 72 hpf and from then on mainly maturation and growth occur, the elevated number and larger size before 72 hpf suggests an important role of EVs during zebrafish organogenesis. Since EVs serve as cargo delivery platforms, the increase in size of sEVs may reflect the need for a higher transport capacity.https://doi.org/10.1186/s12964-025-02053-x |
| spellingShingle | Linda-Marie Mulzer Tim Felger Luis E. Muñoz Gesa Engl Heiko Reutter Mario Schiffer Leila Pourtalebi Jahromi Fanni Annamária Boros Friederike Zunke Philipp Arnold Alina C. Hilger Dynamic changes of extracellular vesicles during zebrafish organogenesis Cell Communication and Signaling |
| title | Dynamic changes of extracellular vesicles during zebrafish organogenesis |
| title_full | Dynamic changes of extracellular vesicles during zebrafish organogenesis |
| title_fullStr | Dynamic changes of extracellular vesicles during zebrafish organogenesis |
| title_full_unstemmed | Dynamic changes of extracellular vesicles during zebrafish organogenesis |
| title_short | Dynamic changes of extracellular vesicles during zebrafish organogenesis |
| title_sort | dynamic changes of extracellular vesicles during zebrafish organogenesis |
| url | https://doi.org/10.1186/s12964-025-02053-x |
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