Distinct Molecular Epidemiology, Transmission Patterns, and Resistance Mutations of HIV-1 Subtypes A1, A6, and A7 in Bulgaria

By 2022, Bulgaria’s National Reference Laboratory had confirmed 4024 HIV cases. We analyzed 132 pol gene sequences to characterize the molecular epidemiology of HIV-1 subtypes A1, A6, and A7 (2001–2022). A1 accounted for 50.0% (66/132) of cases, increasing after 2014, with peaks in 2019 and 2022. A6...

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Main Authors: Aleksandra Partsuneva, Anna Gancheva, Reneta Dimitrova, Lyubomira Grigorova, Asya Kostadinova, Maria Nikolova, Radoslava Emilova, Nina Yancheva, Rusina Grozdeva, Ivailo Alexiev
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Language:English
Published: MDPI AG 2025-05-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/5/1108
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author Aleksandra Partsuneva
Anna Gancheva
Reneta Dimitrova
Lyubomira Grigorova
Asya Kostadinova
Maria Nikolova
Radoslava Emilova
Nina Yancheva
Rusina Grozdeva
Ivailo Alexiev
author_facet Aleksandra Partsuneva
Anna Gancheva
Reneta Dimitrova
Lyubomira Grigorova
Asya Kostadinova
Maria Nikolova
Radoslava Emilova
Nina Yancheva
Rusina Grozdeva
Ivailo Alexiev
author_sort Aleksandra Partsuneva
collection DOAJ
description By 2022, Bulgaria’s National Reference Laboratory had confirmed 4024 HIV cases. We analyzed 132 pol gene sequences to characterize the molecular epidemiology of HIV-1 subtypes A1, A6, and A7 (2001–2022). A1 accounted for 50.0% (66/132) of cases, increasing after 2014, with peaks in 2019 and 2022. A6 comprised 48.5% (64/132), dominating from 2005 to 2014 before stabilizing. A7 was rare (1.5%, 2/132), detected in 2003 and 2011. Transmission patterns varied: A1 was linked to men who have sex with men (MSM) (62.1%), while A6 was primarily heterosexual (HET) (82.8%) with a balanced gender distribution (56.3% male, 43.8% female). Resistance mutations were identified in 29.6% of cases, with A6 showing higher rates of nucleoside reverse transcriptase inhibitor (NRTI) (20.3%) and non-nucleoside reverse transcriptase inhibitor (NNRTI) (7.8%) resistance than A1. Phylogenetic analysis revealed that 13 Bulgarian sequences (9.8%) were involved in transmission clusters, including 10 (7.6%) from sub-subtype A1 and 3 (2.3%) from sub-subtype A6, highlighting distinct genetic diversity and transmission patterns. Despite significant migration from Ukraine in 2022, A6 prevalence remained unchanged, suggesting localized transmission dynamics. These findings highlight a shifting HIV-1 sub-subtype distribution in Bulgaria and emphasize the need for targeted prevention, diagnosis, and treatment strategies tailored to the evolving molecular landscape.
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spelling doaj-art-eefeb48089a44e328ee12c4c09a4b1e72025-08-20T01:56:42ZengMDPI AGMicroorganisms2076-26072025-05-01135110810.3390/microorganisms13051108Distinct Molecular Epidemiology, Transmission Patterns, and Resistance Mutations of HIV-1 Subtypes A1, A6, and A7 in BulgariaAleksandra Partsuneva0Anna Gancheva1Reneta Dimitrova2Lyubomira Grigorova3Asya Kostadinova4Maria Nikolova5Radoslava Emilova6Nina Yancheva7Rusina Grozdeva8Ivailo Alexiev9National Reference Confirmatory Laboratory of HIV, National Center of Infectious and Parasitic Diseases (NCIPD), 1504 Sofia, BulgariaNational Reference Confirmatory Laboratory of HIV, National Center of Infectious and Parasitic Diseases (NCIPD), 1504 Sofia, BulgariaNational Reference Confirmatory Laboratory of HIV, National Center of Infectious and Parasitic Diseases (NCIPD), 1504 Sofia, BulgariaNational Reference Confirmatory Laboratory of HIV, National Center of Infectious and Parasitic Diseases (NCIPD), 1504 Sofia, BulgariaNational Reference Confirmatory Laboratory of HIV, National Center of Infectious and Parasitic Diseases (NCIPD), 1504 Sofia, BulgariaNational Reference Laboratory of Immunology, National Center of Infectious and Parasitic Diseases (NCIPD), 1504 Sofia, BulgariaNational Reference Laboratory of Immunology, National Center of Infectious and Parasitic Diseases (NCIPD), 1504 Sofia, BulgariaSpecialized Hospital for Active Treatment of Infectious and Parasitic Diseases, 1606 Sofia, BulgariaSpecialized Hospital for Active Treatment of Infectious and Parasitic Diseases, 1606 Sofia, BulgariaNational Reference Confirmatory Laboratory of HIV, National Center of Infectious and Parasitic Diseases (NCIPD), 1504 Sofia, BulgariaBy 2022, Bulgaria’s National Reference Laboratory had confirmed 4024 HIV cases. We analyzed 132 pol gene sequences to characterize the molecular epidemiology of HIV-1 subtypes A1, A6, and A7 (2001–2022). A1 accounted for 50.0% (66/132) of cases, increasing after 2014, with peaks in 2019 and 2022. A6 comprised 48.5% (64/132), dominating from 2005 to 2014 before stabilizing. A7 was rare (1.5%, 2/132), detected in 2003 and 2011. Transmission patterns varied: A1 was linked to men who have sex with men (MSM) (62.1%), while A6 was primarily heterosexual (HET) (82.8%) with a balanced gender distribution (56.3% male, 43.8% female). Resistance mutations were identified in 29.6% of cases, with A6 showing higher rates of nucleoside reverse transcriptase inhibitor (NRTI) (20.3%) and non-nucleoside reverse transcriptase inhibitor (NNRTI) (7.8%) resistance than A1. Phylogenetic analysis revealed that 13 Bulgarian sequences (9.8%) were involved in transmission clusters, including 10 (7.6%) from sub-subtype A1 and 3 (2.3%) from sub-subtype A6, highlighting distinct genetic diversity and transmission patterns. Despite significant migration from Ukraine in 2022, A6 prevalence remained unchanged, suggesting localized transmission dynamics. These findings highlight a shifting HIV-1 sub-subtype distribution in Bulgaria and emphasize the need for targeted prevention, diagnosis, and treatment strategies tailored to the evolving molecular landscape.https://www.mdpi.com/2076-2607/13/5/1108HIV-1subtype A1subtype A6subtype A7molecular epidemiologyphylogenetic analysis
spellingShingle Aleksandra Partsuneva
Anna Gancheva
Reneta Dimitrova
Lyubomira Grigorova
Asya Kostadinova
Maria Nikolova
Radoslava Emilova
Nina Yancheva
Rusina Grozdeva
Ivailo Alexiev
Distinct Molecular Epidemiology, Transmission Patterns, and Resistance Mutations of HIV-1 Subtypes A1, A6, and A7 in Bulgaria
Microorganisms
HIV-1
subtype A1
subtype A6
subtype A7
molecular epidemiology
phylogenetic analysis
title Distinct Molecular Epidemiology, Transmission Patterns, and Resistance Mutations of HIV-1 Subtypes A1, A6, and A7 in Bulgaria
title_full Distinct Molecular Epidemiology, Transmission Patterns, and Resistance Mutations of HIV-1 Subtypes A1, A6, and A7 in Bulgaria
title_fullStr Distinct Molecular Epidemiology, Transmission Patterns, and Resistance Mutations of HIV-1 Subtypes A1, A6, and A7 in Bulgaria
title_full_unstemmed Distinct Molecular Epidemiology, Transmission Patterns, and Resistance Mutations of HIV-1 Subtypes A1, A6, and A7 in Bulgaria
title_short Distinct Molecular Epidemiology, Transmission Patterns, and Resistance Mutations of HIV-1 Subtypes A1, A6, and A7 in Bulgaria
title_sort distinct molecular epidemiology transmission patterns and resistance mutations of hiv 1 subtypes a1 a6 and a7 in bulgaria
topic HIV-1
subtype A1
subtype A6
subtype A7
molecular epidemiology
phylogenetic analysis
url https://www.mdpi.com/2076-2607/13/5/1108
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