Research Progress on Immunological Biochips Based on Surface Plasmon Resonance

Biomolecular detection plays essential and irreplaceable roles in safeguarding human health, impeding the transmission of diseases, and augmenting the efficacy of treatments. The precise and specific identification of biomarkers holds profound significance for the early diagnosis, real-time surveill...

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Main Authors: Mengyao Wang, Yangming Hu, Wenjun Zhang, Tianzhu Zhang, Xiaobo He
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Photonics
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Online Access:https://www.mdpi.com/2304-6732/12/4/294
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author Mengyao Wang
Yangming Hu
Wenjun Zhang
Tianzhu Zhang
Xiaobo He
author_facet Mengyao Wang
Yangming Hu
Wenjun Zhang
Tianzhu Zhang
Xiaobo He
author_sort Mengyao Wang
collection DOAJ
description Biomolecular detection plays essential and irreplaceable roles in safeguarding human health, impeding the transmission of diseases, and augmenting the efficacy of treatments. The precise and specific identification of biomarkers holds profound significance for the early diagnosis, real-time surveillance, and targeted treatment of various diseases. In the initial phases of numerous diseases, the absence of distinct biomarkers in the bloodstream often leads to weak detection signals when using traditional immune detection methods such as enzyme-linked immunosorbent assays (ELISAs), chemiluminescence, and fluorescence chromatography. With the surge in research on surface plasmons, innovative approaches have recently emerged that combine surface plasmon resonance (SPR) with immunological detection techniques, reducing the detection sensitivity to 283 ag/mL, shrinking the sensor size to 2.228 µm<sup>2</sup>, and shortening the detection time to 5.5 min. This review provides an overview of the theoretical foundations of surface plasmon resonance and immunoassays and then delves into the latest advancements in biosensors based on these principles, categorizing them according to their detection mechanisms and methodologies. Finally, we discuss future research directions, opportunities, and the challenges hindering the development of highly sensitive immuno-biochips.
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institution OA Journals
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spelling doaj-art-eef87e2898584709ae7a63d0fab153312025-08-20T02:28:41ZengMDPI AGPhotonics2304-67322025-03-0112429410.3390/photonics12040294Research Progress on Immunological Biochips Based on Surface Plasmon ResonanceMengyao Wang0Yangming Hu1Wenjun Zhang2Tianzhu Zhang3Xiaobo He4School of Physics, Henan Normal University, Xinxiang 453007, ChinaInstitute of Physics, Henan Academy of Sciences, Zhengzhou 450046, ChinaInstitute of Physics, Henan Academy of Sciences, Zhengzhou 450046, ChinaInstitute of Physics, Henan Academy of Sciences, Zhengzhou 450046, ChinaInstitute of Physics, Henan Academy of Sciences, Zhengzhou 450046, ChinaBiomolecular detection plays essential and irreplaceable roles in safeguarding human health, impeding the transmission of diseases, and augmenting the efficacy of treatments. The precise and specific identification of biomarkers holds profound significance for the early diagnosis, real-time surveillance, and targeted treatment of various diseases. In the initial phases of numerous diseases, the absence of distinct biomarkers in the bloodstream often leads to weak detection signals when using traditional immune detection methods such as enzyme-linked immunosorbent assays (ELISAs), chemiluminescence, and fluorescence chromatography. With the surge in research on surface plasmons, innovative approaches have recently emerged that combine surface plasmon resonance (SPR) with immunological detection techniques, reducing the detection sensitivity to 283 ag/mL, shrinking the sensor size to 2.228 µm<sup>2</sup>, and shortening the detection time to 5.5 min. This review provides an overview of the theoretical foundations of surface plasmon resonance and immunoassays and then delves into the latest advancements in biosensors based on these principles, categorizing them according to their detection mechanisms and methodologies. Finally, we discuss future research directions, opportunities, and the challenges hindering the development of highly sensitive immuno-biochips.https://www.mdpi.com/2304-6732/12/4/294surface plasmonbiochipimmunological detectionbiomolecular detection
spellingShingle Mengyao Wang
Yangming Hu
Wenjun Zhang
Tianzhu Zhang
Xiaobo He
Research Progress on Immunological Biochips Based on Surface Plasmon Resonance
Photonics
surface plasmon
biochip
immunological detection
biomolecular detection
title Research Progress on Immunological Biochips Based on Surface Plasmon Resonance
title_full Research Progress on Immunological Biochips Based on Surface Plasmon Resonance
title_fullStr Research Progress on Immunological Biochips Based on Surface Plasmon Resonance
title_full_unstemmed Research Progress on Immunological Biochips Based on Surface Plasmon Resonance
title_short Research Progress on Immunological Biochips Based on Surface Plasmon Resonance
title_sort research progress on immunological biochips based on surface plasmon resonance
topic surface plasmon
biochip
immunological detection
biomolecular detection
url https://www.mdpi.com/2304-6732/12/4/294
work_keys_str_mv AT mengyaowang researchprogressonimmunologicalbiochipsbasedonsurfaceplasmonresonance
AT yangminghu researchprogressonimmunologicalbiochipsbasedonsurfaceplasmonresonance
AT wenjunzhang researchprogressonimmunologicalbiochipsbasedonsurfaceplasmonresonance
AT tianzhuzhang researchprogressonimmunologicalbiochipsbasedonsurfaceplasmonresonance
AT xiaobohe researchprogressonimmunologicalbiochipsbasedonsurfaceplasmonresonance