Biodegradable exosome-engineered hydrogels for the prevention of peritoneal adhesions via anti-oxidation and anti-inflammation
Peritoneal adhesions (PA) are a common and severe complication after abdominal surgery, impacting millions of patients worldwide. The use of anti-adhesion materials as physical barriers is an effective strategy to prevent postoperative adhesions. However, the local inflammatory microenvironment exer...
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| Format: | Article |
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Elsevier
2024-12-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006424003739 |
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| author | Weitong Wang Yuchen Ren Qingyu Yu Lijie Jiang Chaojie Yu Zhiwei Yue Yue Wang Jiajun Lu Pengcheng Che Junjie Li Hong Sun |
| author_facet | Weitong Wang Yuchen Ren Qingyu Yu Lijie Jiang Chaojie Yu Zhiwei Yue Yue Wang Jiajun Lu Pengcheng Che Junjie Li Hong Sun |
| author_sort | Weitong Wang |
| collection | DOAJ |
| description | Peritoneal adhesions (PA) are a common and severe complication after abdominal surgery, impacting millions of patients worldwide. The use of anti-adhesion materials as physical barriers is an effective strategy to prevent postoperative adhesions. However, the local inflammatory microenvironment exerts a significant impact on the efficacy of anti-adhesion therapies. In this study, an injectable hydrogel based on oxidized dextran/carboxymethyl chitosan (DCC) is designed and prepared. Furthermore, the DCC hydrogel is specifically engineered to load the adipose mesenchymal stem cells (ADSCs)-derived exosomes (Exos) for the treatment of PA. The prepared DCC hydrogel can act as the physical barrier via covering the irregular wound surface effectively. Moreover, it shows controlled degradation property, enabling the regulated release of Exos. The DCC hydrogel loaded Exos (DCC/Exo) system has high antioxidant capacity, and can effectively modulate the inflammatory microenvironments and diminish apoptosis. Notably, it promotes a polarization shift towards the M2-like phenotype in macrophages. The RNA-seq analysis confirms that the DCC/Exo system exhibits significant anti-inflammatory properties and promotes a reduction in collagen deposition. Consequently, the DCC/Exo system can inhibit peritoneal adhesions significantly in a mouse cecum-abdominal wall injury model. These results demonstrate the DCC/Exo is an ideal material for preventing postoperative adhesions. |
| format | Article |
| id | doaj-art-eee85cac895442d3a840939738208ff9 |
| institution | DOAJ |
| issn | 2590-0064 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-eee85cac895442d3a840939738208ff92025-08-20T02:39:31ZengElsevierMaterials Today Bio2590-00642024-12-012910131210.1016/j.mtbio.2024.101312Biodegradable exosome-engineered hydrogels for the prevention of peritoneal adhesions via anti-oxidation and anti-inflammationWeitong Wang0Yuchen Ren1Qingyu Yu2Lijie Jiang3Chaojie Yu4Zhiwei Yue5Yue Wang6Jiajun Lu7Pengcheng Che8Junjie Li9Hong Sun10Department of Basic Medical Sciences, North China University of Science and Technology, Tangshan, 063210, ChinaDepartment of Basic Medical Sciences, North China University of Science and Technology, Tangshan, 063210, ChinaFrontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, ChinaDepartment of Basic Medical Sciences, North China University of Science and Technology, Tangshan, 063210, ChinaFrontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, ChinaDepartment of Basic Medical Sciences, North China University of Science and Technology, Tangshan, 063210, ChinaDepartment of Basic Medical Sciences, North China University of Science and Technology, Tangshan, 063210, ChinaDepartment of Basic Medical Sciences, North China University of Science and Technology, Tangshan, 063210, ChinaSchool of Nursing and Rehabilitation, North China University of Science and Technology, Tangshan, 063210, China; Corresponding author.Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China; Corresponding author.Department of Basic Medical Sciences, North China University of Science and Technology, Tangshan, 063210, China; Corresponding author.Peritoneal adhesions (PA) are a common and severe complication after abdominal surgery, impacting millions of patients worldwide. The use of anti-adhesion materials as physical barriers is an effective strategy to prevent postoperative adhesions. However, the local inflammatory microenvironment exerts a significant impact on the efficacy of anti-adhesion therapies. In this study, an injectable hydrogel based on oxidized dextran/carboxymethyl chitosan (DCC) is designed and prepared. Furthermore, the DCC hydrogel is specifically engineered to load the adipose mesenchymal stem cells (ADSCs)-derived exosomes (Exos) for the treatment of PA. The prepared DCC hydrogel can act as the physical barrier via covering the irregular wound surface effectively. Moreover, it shows controlled degradation property, enabling the regulated release of Exos. The DCC hydrogel loaded Exos (DCC/Exo) system has high antioxidant capacity, and can effectively modulate the inflammatory microenvironments and diminish apoptosis. Notably, it promotes a polarization shift towards the M2-like phenotype in macrophages. The RNA-seq analysis confirms that the DCC/Exo system exhibits significant anti-inflammatory properties and promotes a reduction in collagen deposition. Consequently, the DCC/Exo system can inhibit peritoneal adhesions significantly in a mouse cecum-abdominal wall injury model. These results demonstrate the DCC/Exo is an ideal material for preventing postoperative adhesions.http://www.sciencedirect.com/science/article/pii/S2590006424003739Peritoneal adhesionsExosomesHydrogelsImmunoregulation |
| spellingShingle | Weitong Wang Yuchen Ren Qingyu Yu Lijie Jiang Chaojie Yu Zhiwei Yue Yue Wang Jiajun Lu Pengcheng Che Junjie Li Hong Sun Biodegradable exosome-engineered hydrogels for the prevention of peritoneal adhesions via anti-oxidation and anti-inflammation Materials Today Bio Peritoneal adhesions Exosomes Hydrogels Immunoregulation |
| title | Biodegradable exosome-engineered hydrogels for the prevention of peritoneal adhesions via anti-oxidation and anti-inflammation |
| title_full | Biodegradable exosome-engineered hydrogels for the prevention of peritoneal adhesions via anti-oxidation and anti-inflammation |
| title_fullStr | Biodegradable exosome-engineered hydrogels for the prevention of peritoneal adhesions via anti-oxidation and anti-inflammation |
| title_full_unstemmed | Biodegradable exosome-engineered hydrogels for the prevention of peritoneal adhesions via anti-oxidation and anti-inflammation |
| title_short | Biodegradable exosome-engineered hydrogels for the prevention of peritoneal adhesions via anti-oxidation and anti-inflammation |
| title_sort | biodegradable exosome engineered hydrogels for the prevention of peritoneal adhesions via anti oxidation and anti inflammation |
| topic | Peritoneal adhesions Exosomes Hydrogels Immunoregulation |
| url | http://www.sciencedirect.com/science/article/pii/S2590006424003739 |
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