Cytoplasmic SALL4-A isoform expression as a diagnostic marker of less aggressive tumor behavior in gastric cancer
Abstract Background Gastric cancer (GC) poses significant challenges globally, ranking fifth in incidence and fourth in cancer-related mortality. SALL4, a stem cell transcription factor with multiple isoforms, includes SALL4-A as its full-length form. This study aims to evaluate the diagnostic poten...
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| Format: | Article |
| Language: | English |
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BMC
2025-02-01
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| Series: | World Journal of Surgical Oncology |
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| Online Access: | https://doi.org/10.1186/s12957-025-03681-w |
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| author | Saeed Rahmani Amirhesam Babajani Maryam Abolhasani Roya Ghods Elham Kalantari Zahra Madjd |
| author_facet | Saeed Rahmani Amirhesam Babajani Maryam Abolhasani Roya Ghods Elham Kalantari Zahra Madjd |
| author_sort | Saeed Rahmani |
| collection | DOAJ |
| description | Abstract Background Gastric cancer (GC) poses significant challenges globally, ranking fifth in incidence and fourth in cancer-related mortality. SALL4, a stem cell transcription factor with multiple isoforms, includes SALL4-A as its full-length form. This study aims to evaluate the diagnostic potential of SALL4-A isoform expression in GC and its clinical significance. Method Immunohistochemical (IHC) analysis was conducted on Tissue Micro Array (TMA) slides from 167 GC patients. Clinicopathological parameters were correlated with SALL4-A expression, and survival analysis was performed. Diagnostic performance was assessed using metrics such as sensitivity, specificity, and area under the curve (AUC). Results SALL4-A exhibited distinct cytoplasmic expression in GC, correlating with lower histological grade (p = 0.003) and TNM stage (p = 0.003), particularly in the intestinal subtype. Diagnostic evaluation showed an AUC of 0.803 for cytoplasmic expression, demonstrating high diagnostic potential. However, SALL4-A expression did not show significant prognostic value. Conclusion Cytoplasmic SALL4-A expression in GC is associated with less aggressive tumor phenotypes and shows promise as a diagnostic marker. Further research is warranted to elucidate its mechanistic role and potential integration into clinical practice. |
| format | Article |
| id | doaj-art-eee7b8d87fa049be94f02fe6413ddcd2 |
| institution | Kabale University |
| issn | 1477-7819 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj-art-eee7b8d87fa049be94f02fe6413ddcd22025-02-09T12:39:50ZengBMCWorld Journal of Surgical Oncology1477-78192025-02-0123111610.1186/s12957-025-03681-wCytoplasmic SALL4-A isoform expression as a diagnostic marker of less aggressive tumor behavior in gastric cancerSaeed Rahmani0Amirhesam Babajani1Maryam Abolhasani2Roya Ghods3Elham Kalantari4Zahra Madjd5Oncopathology Research Center, Iran University of Medical SciencesOncopathology Research Center, Iran University of Medical SciencesOncopathology Research Center, Iran University of Medical SciencesOncopathology Research Center, Iran University of Medical SciencesOncopathology Research Center, Iran University of Medical SciencesOncopathology Research Center, Iran University of Medical SciencesAbstract Background Gastric cancer (GC) poses significant challenges globally, ranking fifth in incidence and fourth in cancer-related mortality. SALL4, a stem cell transcription factor with multiple isoforms, includes SALL4-A as its full-length form. This study aims to evaluate the diagnostic potential of SALL4-A isoform expression in GC and its clinical significance. Method Immunohistochemical (IHC) analysis was conducted on Tissue Micro Array (TMA) slides from 167 GC patients. Clinicopathological parameters were correlated with SALL4-A expression, and survival analysis was performed. Diagnostic performance was assessed using metrics such as sensitivity, specificity, and area under the curve (AUC). Results SALL4-A exhibited distinct cytoplasmic expression in GC, correlating with lower histological grade (p = 0.003) and TNM stage (p = 0.003), particularly in the intestinal subtype. Diagnostic evaluation showed an AUC of 0.803 for cytoplasmic expression, demonstrating high diagnostic potential. However, SALL4-A expression did not show significant prognostic value. Conclusion Cytoplasmic SALL4-A expression in GC is associated with less aggressive tumor phenotypes and shows promise as a diagnostic marker. Further research is warranted to elucidate its mechanistic role and potential integration into clinical practice.https://doi.org/10.1186/s12957-025-03681-wGastric cancerSALL4-ABiomarkersImmunohistochemistryCancer stem cell |
| spellingShingle | Saeed Rahmani Amirhesam Babajani Maryam Abolhasani Roya Ghods Elham Kalantari Zahra Madjd Cytoplasmic SALL4-A isoform expression as a diagnostic marker of less aggressive tumor behavior in gastric cancer World Journal of Surgical Oncology Gastric cancer SALL4-A Biomarkers Immunohistochemistry Cancer stem cell |
| title | Cytoplasmic SALL4-A isoform expression as a diagnostic marker of less aggressive tumor behavior in gastric cancer |
| title_full | Cytoplasmic SALL4-A isoform expression as a diagnostic marker of less aggressive tumor behavior in gastric cancer |
| title_fullStr | Cytoplasmic SALL4-A isoform expression as a diagnostic marker of less aggressive tumor behavior in gastric cancer |
| title_full_unstemmed | Cytoplasmic SALL4-A isoform expression as a diagnostic marker of less aggressive tumor behavior in gastric cancer |
| title_short | Cytoplasmic SALL4-A isoform expression as a diagnostic marker of less aggressive tumor behavior in gastric cancer |
| title_sort | cytoplasmic sall4 a isoform expression as a diagnostic marker of less aggressive tumor behavior in gastric cancer |
| topic | Gastric cancer SALL4-A Biomarkers Immunohistochemistry Cancer stem cell |
| url | https://doi.org/10.1186/s12957-025-03681-w |
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