DNA-encoded dynamic hydrogels for 3D bioprinted cartilage organoids
Articular cartilage, composed of chondrocytes within a dynamic viscoelastic matrix, has limited self-repair capacity, posing a significant challenge for regeneration. Constructing high-fidelity cartilage organoids through three-dimensional (3D) bioprinting to replicate the structure and physiologica...
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| Language: | English |
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Elsevier
2025-04-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425000675 |
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| author | Ziyu Chen Hao Zhang Jingtao Huang Weizong Weng Zhen Geng Mengmeng Li Jiacan Su |
| author_facet | Ziyu Chen Hao Zhang Jingtao Huang Weizong Weng Zhen Geng Mengmeng Li Jiacan Su |
| author_sort | Ziyu Chen |
| collection | DOAJ |
| description | Articular cartilage, composed of chondrocytes within a dynamic viscoelastic matrix, has limited self-repair capacity, posing a significant challenge for regeneration. Constructing high-fidelity cartilage organoids through three-dimensional (3D) bioprinting to replicate the structure and physiological functions of cartilage is crucial for regenerative medicine, drug screening, and disease modeling. However, commonly used matrix bioinks lack reversible cross-linking and precise controllability, hindering dynamic cellular regulation. Thus, encoding bioinks adaptive for cultivating cartilage organoids is an attractive idea. DNA, with its ability to be intricately encoded and reversibly cross-linked into hydrogels, offers precise manipulation at both molecular and spatial structural levels. This endows the hydrogels with viscoelasticity, printability, cell recognition, and stimuli responsiveness. This paper elaborates on strategies to encode bioink via DNA, emphasizing the regulation of predictable dynamic properties and the resulting interactions with cell behavior. The significance of these interactions for the construction of cartilage organoids is highlighted. Finally, we discuss the challenges and future prospects of using DNA-encoded hydrogels for 3D bioprinted cartilage organoids, underscoring their potential impact on advancing biomedical applications. |
| format | Article |
| id | doaj-art-eee6067504b24cde8cc79098621403b0 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-eee6067504b24cde8cc79098621403b02025-01-26T05:04:44ZengElsevierMaterials Today Bio2590-00642025-04-0131101509DNA-encoded dynamic hydrogels for 3D bioprinted cartilage organoidsZiyu Chen0Hao Zhang1Jingtao Huang2Weizong Weng3Zhen Geng4Mengmeng Li5Jiacan Su6Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China; Musculoskeletal Organoid Research Center, Shanghai University, Shanghai, 200444, China; School of Medicine, Shanghai University, Shanghai, 200444, ChinaInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China; Musculoskeletal Organoid Research Center, Shanghai University, Shanghai, 200444, ChinaDepartment of Orthopedics, Shanghai Zhongye Hospital, Shanghai, 201900, ChinaInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China; Musculoskeletal Organoid Research Center, Shanghai University, Shanghai, 200444, China; Corresponding authors. Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China.Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China; Musculoskeletal Organoid Research Center, Shanghai University, Shanghai, 200444, China; Corresponding author. Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China.Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China; Musculoskeletal Organoid Research Center, Shanghai University, Shanghai, 200444, China; Sanming Institute of Translational Medicine, Fujian, 365004, China; Corresponding authors. Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China.Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China; Musculoskeletal Organoid Research Center, Shanghai University, Shanghai, 200444, China; Department of Orthopedics, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Corresponding author. Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China.Articular cartilage, composed of chondrocytes within a dynamic viscoelastic matrix, has limited self-repair capacity, posing a significant challenge for regeneration. Constructing high-fidelity cartilage organoids through three-dimensional (3D) bioprinting to replicate the structure and physiological functions of cartilage is crucial for regenerative medicine, drug screening, and disease modeling. However, commonly used matrix bioinks lack reversible cross-linking and precise controllability, hindering dynamic cellular regulation. Thus, encoding bioinks adaptive for cultivating cartilage organoids is an attractive idea. DNA, with its ability to be intricately encoded and reversibly cross-linked into hydrogels, offers precise manipulation at both molecular and spatial structural levels. This endows the hydrogels with viscoelasticity, printability, cell recognition, and stimuli responsiveness. This paper elaborates on strategies to encode bioink via DNA, emphasizing the regulation of predictable dynamic properties and the resulting interactions with cell behavior. The significance of these interactions for the construction of cartilage organoids is highlighted. Finally, we discuss the challenges and future prospects of using DNA-encoded hydrogels for 3D bioprinted cartilage organoids, underscoring their potential impact on advancing biomedical applications.http://www.sciencedirect.com/science/article/pii/S2590006425000675DNA hydrogelBioprintingCartilage organoidsTissue engineering |
| spellingShingle | Ziyu Chen Hao Zhang Jingtao Huang Weizong Weng Zhen Geng Mengmeng Li Jiacan Su DNA-encoded dynamic hydrogels for 3D bioprinted cartilage organoids Materials Today Bio DNA hydrogel Bioprinting Cartilage organoids Tissue engineering |
| title | DNA-encoded dynamic hydrogels for 3D bioprinted cartilage organoids |
| title_full | DNA-encoded dynamic hydrogels for 3D bioprinted cartilage organoids |
| title_fullStr | DNA-encoded dynamic hydrogels for 3D bioprinted cartilage organoids |
| title_full_unstemmed | DNA-encoded dynamic hydrogels for 3D bioprinted cartilage organoids |
| title_short | DNA-encoded dynamic hydrogels for 3D bioprinted cartilage organoids |
| title_sort | dna encoded dynamic hydrogels for 3d bioprinted cartilage organoids |
| topic | DNA hydrogel Bioprinting Cartilage organoids Tissue engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425000675 |
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