Pandan–vanilla rotation mitigates Fusarium wilt disease in vanilla: insights from rhizosphere microbial community shifts
IntroductionVanilla monoculture often leads to Fusarium wilt disease, affecting the industry globally.MethodsHere, we evaluated the effects of vanilla–black pepper, –pandan, and –sweet rice tea rotations (i.e., growing vanilla in soil previously planted with these crops) on Fusarium oxysporum abunda...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1496701/full |
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| author | Shan Hong Shan Hong Shan Hong Shan Hong Yizhang Xing Jinming Yang Qingyun Zhao Fan Su Huifa Zhuang Hui Wang Zhuangsheng Wu Yisong Chen Yisong Chen |
| author_facet | Shan Hong Shan Hong Shan Hong Shan Hong Yizhang Xing Jinming Yang Qingyun Zhao Fan Su Huifa Zhuang Hui Wang Zhuangsheng Wu Yisong Chen Yisong Chen |
| author_sort | Shan Hong |
| collection | DOAJ |
| description | IntroductionVanilla monoculture often leads to Fusarium wilt disease, affecting the industry globally.MethodsHere, we evaluated the effects of vanilla–black pepper, –pandan, and –sweet rice tea rotations (i.e., growing vanilla in soil previously planted with these crops) on Fusarium oxysporum abundance and rhizosphere microbial communities using real-time quantitative PCR and high-throughput sequencing.ResultsPandan rotation, in particular, reduced disease incidence to 17% and decreased F. oxysporum copy numbers; sweet rice tea showed similar suppressive effects. Crop rotation significantly increased fungal diversity and richness. Different cropping systems, including fallow, monoculture and crop rotation, significantly influenced fungal and bacterial community development, with cropping system and rotated crops being the main drivers of rhizosphere community assembly. The black pepper and pandan rotations specifically enriched certain fungal OTUs, such as OTU1_Thermomyces, OTU37_Arthrobotrys, and OTU18_Arthrobotrys, which serve as biomarkers for the presence of F. oxysporum. After pandan rotation, microbial interactions within the rhizosphere intensified, with notable enrichment of core bacterial taxa, including OTU22_Nitrosospira, OTU56_Lacibacterium, and OTU178_Actinospica. Soil pH was identified as a significant factor influencing microbial community assembly. The fungal community structure, along with core OTU22_Nitrosospira and soil pH, was pivotal in curbing pathogen growth, explaining 25.19%, 8.61%, and 20.45% of the variance, respectively.ConclusionThis study revealed that incorporating pandan into crop rotation may effectively alleviate soil-borne diseases during vanilla production. |
| format | Article |
| id | doaj-art-eee58990a3d6496fbfadad48180f69d0 |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-eee58990a3d6496fbfadad48180f69d02025-08-20T02:35:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-06-011610.3389/fmicb.2025.14967011496701Pandan–vanilla rotation mitigates Fusarium wilt disease in vanilla: insights from rhizosphere microbial community shiftsShan Hong0Shan Hong1Shan Hong2Shan Hong3Yizhang Xing4Jinming Yang5Qingyun Zhao6Fan Su7Huifa Zhuang8Hui Wang9Zhuangsheng Wu10Yisong Chen11Yisong Chen12Sanya Institute, Hainan Academy of Agricultural Sciences/Hainan Key Laboratory of Vegetable Biology, The Institute of Vegetables, Hainan Academy of Agricultural Sciences, Sanya, Hainan Province, ChinaState Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, ChinaHainan Seed Industry Laboratory, Sanya, Hainan Province, ChinaNational Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya, Hainan Province, ChinaSpice and Beverage of Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture and Rural Affairs/Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning, Hainan Province, ChinaThe Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Sanya, Hainan Province, ChinaSpice and Beverage of Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture and Rural Affairs/Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning, Hainan Province, ChinaSpice and Beverage of Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture and Rural Affairs/Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning, Hainan Province, ChinaSpice and Beverage of Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture and Rural Affairs/Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning, Hainan Province, ChinaSpice and Beverage of Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture and Rural Affairs/Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning, Hainan Province, ChinaSanya Institute, Hainan Academy of Agricultural Sciences/Hainan Key Laboratory of Vegetable Biology, The Institute of Vegetables, Hainan Academy of Agricultural Sciences, Sanya, Hainan Province, ChinaSanya Institute, Hainan Academy of Agricultural Sciences/Hainan Key Laboratory of Vegetable Biology, The Institute of Vegetables, Hainan Academy of Agricultural Sciences, Sanya, Hainan Province, ChinaNational Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya, Hainan Province, ChinaIntroductionVanilla monoculture often leads to Fusarium wilt disease, affecting the industry globally.MethodsHere, we evaluated the effects of vanilla–black pepper, –pandan, and –sweet rice tea rotations (i.e., growing vanilla in soil previously planted with these crops) on Fusarium oxysporum abundance and rhizosphere microbial communities using real-time quantitative PCR and high-throughput sequencing.ResultsPandan rotation, in particular, reduced disease incidence to 17% and decreased F. oxysporum copy numbers; sweet rice tea showed similar suppressive effects. Crop rotation significantly increased fungal diversity and richness. Different cropping systems, including fallow, monoculture and crop rotation, significantly influenced fungal and bacterial community development, with cropping system and rotated crops being the main drivers of rhizosphere community assembly. The black pepper and pandan rotations specifically enriched certain fungal OTUs, such as OTU1_Thermomyces, OTU37_Arthrobotrys, and OTU18_Arthrobotrys, which serve as biomarkers for the presence of F. oxysporum. After pandan rotation, microbial interactions within the rhizosphere intensified, with notable enrichment of core bacterial taxa, including OTU22_Nitrosospira, OTU56_Lacibacterium, and OTU178_Actinospica. Soil pH was identified as a significant factor influencing microbial community assembly. The fungal community structure, along with core OTU22_Nitrosospira and soil pH, was pivotal in curbing pathogen growth, explaining 25.19%, 8.61%, and 20.45% of the variance, respectively.ConclusionThis study revealed that incorporating pandan into crop rotation may effectively alleviate soil-borne diseases during vanilla production.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1496701/fullcrop rotationvanillapandanmicrobial communityrhizosphere microbiome |
| spellingShingle | Shan Hong Shan Hong Shan Hong Shan Hong Yizhang Xing Jinming Yang Qingyun Zhao Fan Su Huifa Zhuang Hui Wang Zhuangsheng Wu Yisong Chen Yisong Chen Pandan–vanilla rotation mitigates Fusarium wilt disease in vanilla: insights from rhizosphere microbial community shifts Frontiers in Microbiology crop rotation vanilla pandan microbial community rhizosphere microbiome |
| title | Pandan–vanilla rotation mitigates Fusarium wilt disease in vanilla: insights from rhizosphere microbial community shifts |
| title_full | Pandan–vanilla rotation mitigates Fusarium wilt disease in vanilla: insights from rhizosphere microbial community shifts |
| title_fullStr | Pandan–vanilla rotation mitigates Fusarium wilt disease in vanilla: insights from rhizosphere microbial community shifts |
| title_full_unstemmed | Pandan–vanilla rotation mitigates Fusarium wilt disease in vanilla: insights from rhizosphere microbial community shifts |
| title_short | Pandan–vanilla rotation mitigates Fusarium wilt disease in vanilla: insights from rhizosphere microbial community shifts |
| title_sort | pandan vanilla rotation mitigates fusarium wilt disease in vanilla insights from rhizosphere microbial community shifts |
| topic | crop rotation vanilla pandan microbial community rhizosphere microbiome |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1496701/full |
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