Molecular insights into the functional role of PbNRT2.4 in potassium homeostasis and nutrient transport in Pyrus ussuriensis
Potassium (K), often referred to as the ‘quality element’, is essential for nutrient absorption in fruit trees, with the efficiency of the rootstock's nutrient utilization being a critical factor in the plant's overall nutrient status. Pyrus ussuriensis, identified as a variety with high p...
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Elsevier
2025-03-01
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| Series: | Plant Stress |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X2500017X |
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| author | Han Yang Hao Xu Yujie Shi Liyan Chen Lijuan Zhang Liping Kan Yumeng Jin Xinlan Mei Yangchun Xu Nazir Ahmed Caixia Dong |
| author_facet | Han Yang Hao Xu Yujie Shi Liyan Chen Lijuan Zhang Liping Kan Yumeng Jin Xinlan Mei Yangchun Xu Nazir Ahmed Caixia Dong |
| author_sort | Han Yang |
| collection | DOAJ |
| description | Potassium (K), often referred to as the ‘quality element’, is essential for nutrient absorption in fruit trees, with the efficiency of the rootstock's nutrient utilization being a critical factor in the plant's overall nutrient status. Pyrus ussuriensis, identified as a variety with high potassium efficiency, demonstrates superior tolerance to potassium-deficient conditions and a significant affinity for potassium, compared to Pyrus betulifolia, attributed to its proficient capacity for potassium ion redistribution. This study delves into the molecular mechanisms underlying this high potassium efficiency, focusing on PbNRT2.4 gene, a pivotal regulatory factor in the absorption and translocation of potassium. Predominantly expressed in the roots, PbNRT2.4, is finely regulated by potassium concentrations, as well as exogenous sugar levels. Under K+ limitation, sucrose and sorbitol application significantly upregulates PbNRT2.4 expression in P. ussuriensis, thereby enhancing K+ absorption. In yeast systems, PbNRT2.4 facilitates K+ uptake, and its overexpression in hairy root systems, particularly in P. ussuriensis, leads to a marked increase in K+ influx in the root and xylem. Overexpression of this gene in pear callus tissues similarly increased intracellular K+ levels under K-deficient conditions. Interactions among PbNRT2.4, PbHAK11, and PbSDH1 proteins, elucidated using yeast two-hybrid, BiFC, and Co-IP assays, are crucial for modulating carbon and nitrogen metabolic processes, thereby harmonizing K+ absorption and transport. These findings provide a detailed understanding of potassium homeostasis at a molecular level. |
| format | Article |
| id | doaj-art-eee5772c5262493caccd830006ec64f7 |
| institution | Kabale University |
| issn | 2667-064X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj-art-eee5772c5262493caccd830006ec64f72025-02-03T04:17:06ZengElsevierPlant Stress2667-064X2025-03-0115100752Molecular insights into the functional role of PbNRT2.4 in potassium homeostasis and nutrient transport in Pyrus ussuriensisHan Yang0Hao Xu1Yujie Shi2Liyan Chen3Lijuan Zhang4Liping Kan5Yumeng Jin6Xinlan Mei7Yangchun Xu8Nazir Ahmed9Caixia Dong10Jiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering, PR China; Center of Resource-saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR China; Xianghu Laboratory, Hangzhou, 311231, PR ChinaJiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering, PR China; Center of Resource-saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR ChinaJiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering, PR China; Center of Resource-saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR ChinaJiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering, PR China; Center of Resource-saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR ChinaJiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering, PR China; Center of Resource-saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR ChinaJiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering, PR China; Center of Resource-saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR ChinaJiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering, PR China; Center of Resource-saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR ChinaJiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering, PR China; Center of Resource-saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR ChinaJiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering, PR China; Center of Resource-saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR ChinaCollege of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510550, PR ChinaJiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering, PR China; Center of Resource-saving Fertilizers, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR China; Corresponding author.Potassium (K), often referred to as the ‘quality element’, is essential for nutrient absorption in fruit trees, with the efficiency of the rootstock's nutrient utilization being a critical factor in the plant's overall nutrient status. Pyrus ussuriensis, identified as a variety with high potassium efficiency, demonstrates superior tolerance to potassium-deficient conditions and a significant affinity for potassium, compared to Pyrus betulifolia, attributed to its proficient capacity for potassium ion redistribution. This study delves into the molecular mechanisms underlying this high potassium efficiency, focusing on PbNRT2.4 gene, a pivotal regulatory factor in the absorption and translocation of potassium. Predominantly expressed in the roots, PbNRT2.4, is finely regulated by potassium concentrations, as well as exogenous sugar levels. Under K+ limitation, sucrose and sorbitol application significantly upregulates PbNRT2.4 expression in P. ussuriensis, thereby enhancing K+ absorption. In yeast systems, PbNRT2.4 facilitates K+ uptake, and its overexpression in hairy root systems, particularly in P. ussuriensis, leads to a marked increase in K+ influx in the root and xylem. Overexpression of this gene in pear callus tissues similarly increased intracellular K+ levels under K-deficient conditions. Interactions among PbNRT2.4, PbHAK11, and PbSDH1 proteins, elucidated using yeast two-hybrid, BiFC, and Co-IP assays, are crucial for modulating carbon and nitrogen metabolic processes, thereby harmonizing K+ absorption and transport. These findings provide a detailed understanding of potassium homeostasis at a molecular level.http://www.sciencedirect.com/science/article/pii/S2667064X2500017XPotassium utilization efficiency (KUE)PbNRT2.4Pyrus ussuriensisNutrient uptakeNitrogen translocationSucrose and sorbitol |
| spellingShingle | Han Yang Hao Xu Yujie Shi Liyan Chen Lijuan Zhang Liping Kan Yumeng Jin Xinlan Mei Yangchun Xu Nazir Ahmed Caixia Dong Molecular insights into the functional role of PbNRT2.4 in potassium homeostasis and nutrient transport in Pyrus ussuriensis Plant Stress Potassium utilization efficiency (KUE) PbNRT2.4 Pyrus ussuriensis Nutrient uptake Nitrogen translocation Sucrose and sorbitol |
| title | Molecular insights into the functional role of PbNRT2.4 in potassium homeostasis and nutrient transport in Pyrus ussuriensis |
| title_full | Molecular insights into the functional role of PbNRT2.4 in potassium homeostasis and nutrient transport in Pyrus ussuriensis |
| title_fullStr | Molecular insights into the functional role of PbNRT2.4 in potassium homeostasis and nutrient transport in Pyrus ussuriensis |
| title_full_unstemmed | Molecular insights into the functional role of PbNRT2.4 in potassium homeostasis and nutrient transport in Pyrus ussuriensis |
| title_short | Molecular insights into the functional role of PbNRT2.4 in potassium homeostasis and nutrient transport in Pyrus ussuriensis |
| title_sort | molecular insights into the functional role of pbnrt2 4 in potassium homeostasis and nutrient transport in pyrus ussuriensis |
| topic | Potassium utilization efficiency (KUE) PbNRT2.4 Pyrus ussuriensis Nutrient uptake Nitrogen translocation Sucrose and sorbitol |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X2500017X |
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